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Occurrence and aetiology of subclinical mastitis in water buffalo in Bangladesh

Published online by Cambridge University Press:  20 August 2021

Shuvo Singha ,
Carl David Ericsson ,
Salma Chowdhury ,
Sanjib Chandra Nath ,
Ovirup Bhushan Paul ,
Md. Ahasanul Hoque ,
Sofia Boqvist ,
Ylva Persson  and
Md. Mizanur Rahman
Shuvo Singha*
Affiliation:
Department of Veterinary Medicine, University of Milan, Lodi, Italy Department of Physiology, Sylhet Agricultural University, Sylhet, Bangladesh Department of Medicine and Surgery, Chattogram Veterinary and Animal Sciences University, Chattogram, Bangladesh Participating Partner, Udder Health Bangladesh, Chattogram, Bangladesh
Carl David Ericsson
Affiliation:
Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden
Salma Chowdhury
Affiliation:
Department of Medicine and Surgery, Chattogram Veterinary and Animal Sciences University, Chattogram, Bangladesh Participating Partner, Udder Health Bangladesh, Chattogram, Bangladesh
Sanjib Chandra Nath
Affiliation:
Department of Medicine and Surgery, Chattogram Veterinary and Animal Sciences University, Chattogram, Bangladesh Participating Partner, Udder Health Bangladesh, Chattogram, Bangladesh
Ovirup Bhushan Paul
Affiliation:
Department of Medicine and Surgery, Chattogram Veterinary and Animal Sciences University, Chattogram, Bangladesh Participating Partner, Udder Health Bangladesh, Chattogram, Bangladesh
Md. Ahasanul Hoque
Affiliation:
Department of Medicine and Surgery, Chattogram Veterinary and Animal Sciences University, Chattogram, Bangladesh Participating Partner, Udder Health Bangladesh, Chattogram, Bangladesh
Sofia Boqvist
Affiliation:
Participating Partner, Udder Health Bangladesh, Chattogram, Bangladesh Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden
Ylva Persson
Affiliation:
Participating Partner, Udder Health Bangladesh, Chattogram, Bangladesh Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute, Uppsala, Sweden
Md. Mizanur Rahman
Affiliation:
Department of Medicine and Surgery, Chattogram Veterinary and Animal Sciences University, Chattogram, Bangladesh Participating Partner, Udder Health Bangladesh, Chattogram, Bangladesh
*
Author for correspondence: Shuvo Singha, Email: shuvo.singha@unimi.it
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Abstract

Subclinical mastitis (SCM) in water buffalo is a production disease associated with decreased milk yield and impaired milk quality and safety. Water buffalo is an important livestock species in Bangladesh, but information about the occurrence and aetiology of SCM in this species is scarce. A cross-sectional study was conducted as part of the Udder Health Bangladesh Programme to (i) determine the occurrence of SCM and bulk milk somatic cell count (SCC) in water buffalo in Bangladesh, (ii) identify pathogens causing SCM and (iii) evaluate penicillin resistance in isolated staphylococci strains. Sixteen buffalo farms in the Bagerhat and Noakhali regions of Bangladesh were selected for study and a bulk milk sample was collected from each farm. In addition, 299 udder quarter milk samples were collected from 76 animals. The bulk milk samples were assessed by direct SCC and the quarter milk samples by California mastitis test (CMT). The occurrence of SCM calculated at quarter and animal level was 42.5 and 81.6%, respectively. Milk samples from 108 CMT-positive quarters in 48 animals and 38 randomly selected CMT-negative quarters in 24 animals were investigated using bacteriological culture. Estimated mean bulk milk SCC was 195 000 cells/ml milk (range 47 000– 587 000 cells/ml milk). On culture, estimated quarter-level intramammary infection (IMI) was 40.4%. The identity of isolated bacteria was confirmed by MALDI-TOF mass spectrometry. Non-aureus staphylococci (NAS) were the most common pathogens (24.7%) and, among 36 NAS tested, 36.1% were resistant to penicillin. Thus there was high occurrence of SCM on the study farms, with relatively high penicillin resistance in NAS. Further studies are needed to identify underlying risk factors and develop an udder health control strategy for water buffalo in Bangladesh.

Keywords

CMTintramammary infectionMALDI-TOFpathogenpenicillinase testSCC
Type
Research Article
Information
Journal of Dairy Research , Volume 88 , Issue 3 , August 2021 , pp. 314 - 320
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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