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Proliferative effect of whey from cows' milk obtained at two different stages of pregnancy measured in MCF-7 cells

  • Tina S Nielsen (a1), Charlotte Andersen (a2), Kris Sejrsen (a1) and Stig Purup (a1)
Abstract

Dietary estrogens may play a role in the etiology of hormone-dependent cancers like breast cancer. Cow's milk contains various endogenous estrogens and feed derived phytoestrogens that potentially contribute to an estrogenic effect of milk in consumers, and therefore we evaluated the effect of milk (whey) in a proliferation assay with estrogen-sensitive MCF-7 human breast cancer cells. Milk samples were obtained from 22 cows representing different stages of pregnancy (first and second half) and whey was produced from the milk. 0·1, 0·25 or 0·5% whey was included in the cell culture medium and after 6 days of treatment cell proliferation was assessed by a colorimetric method with a fluorometer. Whey induced significant (P<0·05) proliferative effects compared with control cells with no added whey at all concentrations tested. There was no difference in the proliferative effect of whey depending on the stage of pregnancy from which the milk was obtained. We did not observe anti-proliferative effects when whey was tested in the presence of 10 pm estradiol in the medium. In conclusion, these results indicate that whey, irrespective of the pregnancy stage from which the milk was obtained induced a significant proliferative response in MCF-7 cells and no anti-proliferative effect, which may be caused, at least in part, by estrogens present in milk. The implications of our findings in relation to for example breast cancer will have to be studied further in other model systems preferentially in vivo.

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Corresponding author
*For correspondence; e-mail: TinaS.Nielsen@agrsci.dk
References
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Abeyawardene SA, Hathorn DJ & Glencross RG 1984 Concentrations of oestradiol-17 beta and progesterone in bovine plasma and defatted milk during the post-partum anovulatory period, during oestrous cycles and following ovariectomy. British Veterinary Journal 140 458467
Andersen C, Nielsen TS, Purup S, Kristensen T, Eriksen J, Søegaard K, Sørensen J & Fretté XC 2009 Phyto-oestrogens in herbage and milk from cows grazing white clover, red clover, lucerne or chicory-rich pastures. Animal 3 11891195
Andersen C, Weisbjerg MR, Hansen-Møller J & Sejrsen K 2008 Effect of forage on the content of phyto-oestrogens in bovine milk. Animal 1 16
Andersson AM & Skakkebaek NE 1999 Exposure to exogenous estrogens in food: possible impact on human development and health. European Journal of Endocrinology 140 477485
Antignac JP, Cariou R, Bizec BL & Andre F 2004 New data regarding phytoestrogens content in bovine milk. Food Chemistry 87 275281
Boyd NF, Martin LJ, Noffel M, Lockwood GA & Trichler DL 1993 A meta-analysis of studies of dietary fat and breast cancer risk. British Journal of Cancer 68 627636
Cabanes A, Wang M, Olivo S, De Assis S, Gustafsson JA, Khan G & Hilakivi-Clarke L 2004 Prepubertal estradiol and genistein exposures up-regulate BRCA1 mRNA and reduce mammary tumorigenesis. Carcinogenesis 25 741748
Chen FP, Chien MH, Chen HY & Ng YT 2011 Effects of different progestogens on human breast tumor cell growth. Climacteric 14 345351
Cho E, Spiegelman D, Hunter DJ, Chen WY, Stampfer MJ, Colditz GA & Willett WC 2003 Premenopausal fat intake and risk of breast cancer. JNCI Journal of the National Cancer Institute 95 10791085
Courant F, Antignac JP, Maume D, Monteau F, Andre F & Le BB 2007 Determination of naturally occurring oestrogens and androgens in retail samples of milk and eggs. Food Additives and Contaminants 24 13581366
Diel P, Schmidt S & Vollmer G 2002 In vivo test systems for the quantitative and qualitative analysis of the biological activity of phytoestrogens. Journal of Chromatography B 777 191202
Dip R, Lenz S, Antignac JP, Le Bizec B, Gmuender H & Naegeli H 2008 Global gene expression profiles induced by phytoestrogens in human breast cancer cells. Endocrine-Related Cancer 15 161173
Dupont J & Le RD 2001 Insulin-like growth factor 1 and oestradiol promote cell proliferation of MCF-7 breast cancer cells: new insights into their synergistic effects. Molecular Pathology 54 149154
Farlow DW, Xu X & Veenstra TD 2009 Quantitative measurement of endogenous estrogen metabolites, risk-factors for development of breast cancer, in commercial milk products by LC-MS/MS. Journal of Chromatography B 877 13271334
Fritsche S & Steinhart H 1999 Occurrence of hormonally active compounds in food: a review. European Food Research and Technology 209 153179
Gaard M, Tretli S & Loken EB 1995 Dietary fat and the risk of breast cancer: a prospective study of 25 892 Norwegian women. International Journal of Cancer 63 1217
Ganmaa D & Sato A 2005 The possible role of female sex hormones in milk from pregnant cows in the development of breast, ovarian and corpus uteri cancers. Medical Hypothesis 65 10281037
Ganmaa D, Tezuka H, Enkhmaa D, Hoshi K & Sato A 2006 Commercial cows’ milk has uterotrophic activity on the uteri of young ovariectomized rats and immature rats. International Journal of Cancer 118 23632365
Gutendorf B & Westendorf J 2001 Comparison of an array of in vitro assays for the assessment of the estrogenic potential of natural and synthetic estrogens, phytoestrogens and xenoestrogens. Toxicology 166 7989
Hartmann S, Lacorn M & Steinhart H 1998 Natural occurrence of steroid hormones in food. Food Chemistry 62 720
Hilakivi-Clarke L, Cabanes A, Olivo S, Kerr L, Bouker KB & Clarke R 2002 Do estrogens always increase breast cancer risk? Journal of Steroid Biochemistry and Molecular Biology 80 163174
Hoikkala A, Mustonen E, Saastamoinen I, Jokela T, Taponen J, Saloniemi H & Wahala K 2007 High levels of equol in organic skimmed Finnish cow milk. Molecular Nutrition and Food Research 51 782786
Ma DF, Katoh R, Zhou H & Wang PY 2007 Promoting effects of milk on the development of 7,12-dimethylbenz(a)anthracene (DMBA)-induced mammary tumors in rats. Acta Histochemica et Cytochemica 40 6167
Malekinejad H, Scherpenisse P & Bergwerff AA 2006 Naturally occurring estrogens in processed milk and in raw milk (from gestated cows). Journal of Agricultural and Food Chemistry 54 97859791
Maruyama K, Oshima T & Ohyama K 2010 Exposure to exogenous estrogen through intake of commercial milk produced from pregnant cows. Pediatrics International 52 3338
Nielsen TS, Khan G, Davis J, Michels KB & Hilakivi-Clarke L 2011 Prepubertal exposure to cow's milk reduces susceptibility to carcinogen-induced mammary tumorigenesis in rats. International Journal of Cancer 128 1220
Nielsen TS, Norgaard JV, Purup S, Frette XC & Bonefeld-Jorgensen EC 2009 Estrogenic activity of bovine milk high or low in equol using immature mouse uterotrophic responses and an estrogen receptor transactivation assay. Cancer Epidemiology 33 6168
Pape-Zambito DA, Magliaro AL & Kensinger RS 2007 Concentrations of 17-beta estradiol in Holstein whole milk. Journal of Dairy Science 90 33083313
Qin L, Xu J, Wang P, Ganmaa D, Li J, Wang J, Kaneko T, Hoshi K, Shirai T & Sato A 2004 Low-fat milk promotes the development of 7,12-dimethylbenz(A)anthracene (DMBA)-induced mammary tumors in rats. International Journal of Cancer 110 491496
Qin LQ, Xu JY, Tezuka H, Li J, Arita J, Hoshi K & Sato A 2007 Consumption of commercial whole and non-fat milk increases the incidence of 7,12-dimethylbenz(a)anthracene-induced mammary tumors in rats. Cancer Detection and Prevention 31 339343
Qin LQ, Xu JY, Tezuka H, Wang PY & Hoshi K 2008 Milk inhibits the regression of 7,12-dimethylbenz(a)anthracene-induced mammary tumors in ovariectomized rats. Nutrition and Cancer 60 505510
Rasmussen TH, Nielsen F, Andersen HR, Nielsen JB, Weihe P & Grandjean P 2003 Assessment of xenoestrogenic exposure by a biomarker approach: application of the E-Screen bioassay to determine estrogenic response of serum extracts. Environmental Health 2 12
Steinshamn H, Purup S, Thuen E & Hansen-Moller J 2008 Effects of clover-grass silages and concentrate supplementation on the content of phytoestrogens in dairy cow milk. Journal of Dairy Science 91 27152725
Tso J & Aga DS 2010 A systematic investigation to optimize simultaneous extraction and liquid chromatography tandem mass spectrometry analysis of estrogens and their conjugated metabolites in milk. Journal of Chromatography A 1217 47844795
Vicini J, Etherton T, Kris-Etherton P, Ballam J, Denham S, Staub R, Goldstein D, Cady R, McGrath M & Lucy M 2008 Survey of retail milk composition as affected by label claims regarding farm-management practices. Journal of the American Dietetic Association 108 11981203
Wang C & Kurzer MS 1997 Phytoestrogen concentration determines effects on DNA synthesis in human breast cancer cells. Nutrition and Cancer 28 236247
Wang C & Kurzer MS 1998 Effects of phytoestrogens on DNA synthesis in MCF-7 cells in the presence of estradiol or growth factors. Nutrition and Cancer 31 90100
Zhou H, Qin LQ, Ma DF, Wang Y & Wang PY 2010 Uterotrophic effects of cow milk in immature ovariectomized Sprague–Dawley rats. Environmental Health Preventive Medicine 15 162168
Zhou H, Qin LQ, Tang FL, Ma DF, Wang PY & Wang Y 2007 Effect of milk on the 7,12-dimethylbenz[a]-anthracene-induced mammary tumor model in rat. Food Chemistry and Toxicology 45 18681872
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Journal of Dairy Research
  • ISSN: 0022-0299
  • EISSN: 1469-7629
  • URL: /core/journals/journal-of-dairy-research
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