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Child's homocysteine concentration at 2 years is influenced by pregnancy vitamin B12 and folate status

  • H. G. Lubree (a1), P. A. Katre (a2), S. M. Joshi (a1), D. S. Bhat (a1), U. S. Deshmukh (a1), N. S. Memane (a1), S. R. Otiv (a1), E. C. Rush (a3) and C. S. Yajnik (a1)...
Abstract

Longitudinal studies investigating vitamin B12 and folate status of mothers and their offspring will provide a better understanding of intergenerational nutrition. During pregnancy and 2 years (2y) after delivery, we measured plasma vitamin B12 and folate concentrations in 118 women [aged (mean ± s.d.) 22.9 ± 3.9y] who attended a rural (n = 68) or an urban (n = 50) antenatal clinic in Pune, India. Cord blood vitamin B12 and folate were measured, and when the child was 2y total homocysteine (tHcy) was also measured. Demographic and diet measurements were recorded using standard methods.

Pregnancy plasma vitamin B12 concentration at 34 weeks was low [median (25th, 75th), 115 (95, 147) pm]; 75% had low status (<150 pm). Plasma folate was high (mean ± s.d., 33 ± 21 nm); one had a folate concentration <7 pm. Cord plasma vitamin B12 and folate concentrations were higher than and positively associated with maternal concentrations. In stepwise regression, higher child vitamin B12 at 2y was predicted (total R 2 15.7%) by pregnancy vitamin B12 (std β 0.201, R 2 7.7%), current consumption of cow's milk (std β 0.194, R 2 3.3%) and whether breast feeding was stopped before 2y (std β −0.234 R 2 7.2%). Child's 2y tHcy concentration was high (11.4 ± 3.6 μm) and predicted by lower pregnancy vitamin B12 (std β −0.206, R 2 4.1%), lack of vitamin supplementation (std β −0.256, R 2 5.6%) in pregnancy and whether currently breastfed (std β 0.268, R 2 8.4%).

Low maternal vitamin B12 status in pregnancy and prolonged breast-feeding results in disturbed one-carbon metabolism in offspring at 2y. Supplementation of women of child-bearing age, particularly during pregnancy and lactation, may improve the homocysteine status of these children.

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Corresponding author
*Address for correspondence: Prof. C. S. Yajnik, Director, Diabetes Unit, KEM Hospital, Rasta Peth, Pune 411011, India. (E-mails diabetes@vsnl.com, csyajnik@hotmail.com)
References
Hide All
1. BohnsackBL, HirschiKK. Nutrient regulation of cell cycle progression. Annu Rev Nutr. 2004; 24, 433453.
2. AshworthCJ, AntipatisC. Micronutrient programming of development throughout gestation. Reproduction. 2001; 122, 527535.
3. AllenLH. Causes of vitamin B12 and folate deficiency. Food Nutr Bull. 2008; 29, S20S34; discussion S35–S27.
4. YajnikCS, DeshpandeSS, JacksonAA, et al. . Vitamin B12 and folate concentrations during pregnancy and insulin resistance in the offspring: the Pune Maternal Nutrition Study. Diabetologia. 2008; 51, 2938.
5. YajnikCS, LubreeHG, ThuseNV, et al. . Oral vitamin B12 supplementation reduces plasma total homocysteine concentration in women in India. Asia Pac J Clin Nutr. 2007; 16, 103109.
6. AntonyAC. Vegetarianism and vitamin B-12 (cobalamin) deficiency. Am J Clin Nutr. 2003; 78, 36.
7. ChambersJC, ObeidOA, RefsumH, et al. . Plasma homocysteine concentrations and risk of coronary heart disease in UK Indian Asian and European men. Lancet. 2000; 355, 523527.
8. DrorDK, AllenLH. Effect of vitamin B12 deficiency on neurodevelopment in infants: current knowledge and possible mechanisms. Nutr Rev. 2008; 66, 250255.
9. MisraA, VikramNK, PandeyRM, et al. . Hyperhomocysteinemia, and low intakes of folic acid and vitamin B12 in urban North India. Eur J Nutr. 2002; 41, 6877.
10. RefsumH, YajnikCS, GadkariM, et al. . Hyperhomocysteinemia and elevated methylmalonic acid indicate a high prevalence of cobalamin deficiency in Asian Indians. Am J Clin Nutr. 2001; 74, 233241.
11. YajnikCS, DeshpandeSS, LubreeHG, et al. . Vitamin B12 deficiency and hyperhomocysteinemia in rural and urban Indians. J Assoc Physicians India. 2006; 54, 775782.
12. RayJG, WyattPR, ThompsonMD, et al. . Vitamin B12 and the risk of neural tube defects in a folic-acid-fortified population. Epidemiology. 2007; 18, 362366.
13. RonnenbergAG, GoldmanMB, ChenD, et al. . Preconception homocysteine and B vitamin status and birth outcomes in Chinese women. Am J Clin Nutr. 2002; 76, 13851391.
14. MuthayyaS, KurpadAV, DugganCP, et al. . Low maternal vitamin B12 status is associated with intrauterine growth retardation in urban South Indians. Eur J Clin Nutr. 2006; 60, 791801.
15. SchneiderH, MillerRK. Receptor-mediated uptake and transport of macromolecules in the human placenta. Int J Dev Biol. 2010; 54, 367375.
16. TanejaS, BhandariN, StrandTA, et al. . Cobalamin and folate status in infants and young children in a low-to-middle income community in India. Am J Clin Nutr. 2007; 86, 13021309.
17. BhateV, DeshpandeS, BhatD, et al. . Vitamin B12 status of pregnant Indian women and cognitive function in their 9-year-old children. Food Nutr Bull. 2008; 29, 249254.
18. MuthayyaS, DwarkanathP, MhaskarM, et al. . The relationship of neonatal serum vitamin B12 status with birth weight. Asia Pac J Clin Nutr. 2006; 15, 538543.
19. KatreP, BhatD, LubreeH, et al. . Vitamin B12 and folic acid supplementation and plasma total homocysteine concentrations in pregnant Indian women with low B12 and high folate status. Asia Pac J Clin Nutr. 2010; 19, 335343.
20. Department of Biometry and Nutrition. Nutritive Values of some Indian Food Preparations, 1992. Maharashtra Association for the Cultivation of Science, Agharkar Research Institute, Pune, India.
21. National Institute of Nutrition. Nutritive Value of Indian Foods, 1989. Indian Council of Medical Research, Hyderabad, India.
22. International Institute for Population Sciences and ORC Macro. National Family Health Survey (NHFS-2), India 1998–99, Mumbai, 2001, pp. 52–57.
23. KelleherBP, BroinSD. Microbiological assay for vitamin B12 performed in 96-well microtitre plates. J Clin Pathol. 1991; 44, 592595.
24. KelleherBP, WalsheKG, ScottJM, O'BroinSD. Microbiological assay for vitamin B12 with use of a colistin–sulfate-resistant organism. Clin Chem. 1987; 33, 5254.
25. HorneDW, PattersonD. Lactobacillus casei microbiological assay of folic acid derivatives in 96-well microtiter plates. Clin Chem. 1988; 34, 23572359.
26. TamuraT, FreebergLE, CornwellPE. Inhibition of EDTA of growth of Lactobacillus casei in the folate microbiological assay and its reversal by added manganese or iron. Clin Chem. 1990; 36, 1993.
27. AlbertiKG, ZimmetPZ. Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: diagnosis and classification of diabetes mellitus provisional report of a WHO consultation. Diabet Med. 1998; 15, 539553.
28. World Health Organization (WHO). WHO Child Growth Standards: Length/Height-for-Age, Weight-for-Length, Weight-for-Height and Body Mass Index-for-Age: Methods and Development, 2006. World Health Organization, Geneva.
29. ElmadfaI, SingerI. Vitamin B-12 and homocysteine status among vegetarians: a global perspective. Am J Clin Nutr. 2009; 89, 1693S1698S.
30. WallaceJM, BonhamMP, StrainJ, et al. . Homocysteine concentration, related B vitamins, and betaine in pregnant women recruited to the Seychelles Child Development Study. Am J Clin Nutr. 2008; 87, 391397.
31. Guerra-ShinoharaEM, PaivaAA, RondoPH, et al. . Relationship between total homocysteine and folate levels in pregnant women and their newborn babies according to maternal serum levels of vitamin B12. BJOG. 2002; 109, 784791.
32. HayG, JohnstonC, WhitelawA, TryggK, RefsumH. Folate and cobalamin status in relation to breastfeeding and weaning in healthy infantsAm J Clin Nutr. 2008; 88, 105114.
33. SpeckerBL, BlackA, AllenL, MorrowF. Vitamin B-12: low milk concentrations are related to low serum concentrations in vegetarian women and to methylmalonic aciduria in their infants. Am J Clin Nutr. 1990; 52, 10731076.
34. RefsumH, SmithAD, UelandPM, et al. . Facts and recommendations about total homocysteine determinations: an expert opinion. Clin Chem. 2004; 50, 332.
35. HayG, TryggK, WhitelawA, JohnstonC, RefsumH. Folate and cobalamin status in relation to diet in healthy 2-y-old children. Am J Clin Nutr. 2011; 93, 727735.
36. StangerO, WegerM. Interactions of homocysteine, nitric oxide, folate and radicals in the progressively damaged endothelium. Clin Chem Lab Med. 2003; 41, 14441454.
37. HayG, ClausenT, WhitelawA, et al. . Maternal folate and cobalamin status predicts vitamin status in newborns and 6-month-old infants. J Nutr. 2010; 140, 557564.
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