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Vitamin B12 and folate during pregnancy and offspring motor, mental and social development at 2 years of age

  • V. K. Bhate (a1), S. M. Joshi (a1), R. S. Ladkat (a1), U. S. Deshmukh (a1), H. G. Lubree (a1), P. A. Katre (a2), D. S. Bhat (a1), E. C. Rush (a3) and C. S. Yajnik (a1)...
Abstract

Insufficiency of vitamin B12 (B12) and folate during pregnancy can result in low concentrations in the fetus and have adverse effects on brain development. We investigated the relationship between maternal B12 and folate nutrition during pregnancy and offspring motor, mental and social development at two years of age (2 y). Mothers (n = 123) and their offspring (62 girls, 61 boys) from rural and middle-class urban communities in and around Pune city were followed through pregnancy up to 2 y. Maternal B12 and folate concentrations were measured at 28 and 34 weeks of gestation. At 2 y, the Developmental Assessment Scale for Indian Infants was used to determine motor and mental developmental quotients and the Vineland Social Maturity Scale for the social developmental quotient. Overall, 62% of the mothers had low B12 levels (<150 pmol/l) and one mother was folate deficient during pregnancy. Maternal B12 at 28 and 34 weeks of gestation was associated with offspring B12 at 2 y (r = 0.29, r = 0.32, P < 0.001), but folate was not associated with offspring folate. At 2 y, motor development was associated with maternal folate at 28 and 34 weeks of gestation. Mental and social development quotients were associated positively with head circumference and negatively with birth weight. In addition, pregnancy B12 and folate were positively associated with mental and social development quotients. Maternal B12 and folate during intrauterine life may favorably influence brain development and function. Pregnancy provides a window of opportunity to enhance fetal psychomotor (motor and mental) development.

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Corresponding author
*Address for correspondence: Prof. C. S. Yajnik, Director, Diabetes Unit, KEM Hospital, Rasta Peth, Pune 411011, India. (Email diabetes@vsnl.com/csyajnik@hotmail.com)
References
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1. FernstromJD. Can nutrient supplements modify brain function? Am J Clin Nutr. 2000; 71 (Suppl 6), 166 9S1675S.
2. GeorgieffMK. Nutrition and the developing brain: nutrient priorities and measurement. Am J Clin Nutr. 2007; 85, 614S620S.
3. LovbladK, RamelliG, RemondaL, et al. Retardation of myelination due to dietary vitamin B12 deficiency: cranial MRI findings. Pediatr Radiol. 1997; 27, 155158.
4. DrorDK, AllenLH. Effect of vitamin B12 deficiency on neurodevelopment in infants: current knowledge and possible mechanisms. Nutr Rev. 2008; 66, 250255.
5. VeenaSR, KrishnaveniGV, SrinivasanK, et al. Higher maternal plasma folate but not vitamin B-12 concentrations during pregnancy are associated with better cognitive function scores in 9- to 10- year-old children in South India. J Nutr. 2010; 140, 10141022.
6. BhateV, DeshpandeS, BhatDS, et al. Vitamin B12 status of pregnant Indian women and cognitive function in their 9-year-old children. Food & Nutr Bull. 2008; 29, 249254.
7. Del Rio GarciaC, Torres-SanchezL, ChenJ, et al. Maternal MTHFR 677C > T genotype and dietary intake of folate and vitamin B(12): their impact on child neurodevelopment. Nutr Neurosci. 2009; 12, 1320.
8. CraciunescuCN, BrownEC, MarMH, et al. Folic acid deficiency during late gestation decreases progenitor cell proliferation and increases apoptosis in fetal mouse brain. J Nutr. 2004; 134, 162166.
9. WhitleyJR, O'DellBL, HoganAG. Effect of diet on maze learning in second generation rats; folic acid deficiency. J Nutr. 1951; 45, 153160.
10. International Institute for Population Sciences. Third National Family Health Survey NHFS-3, India 2005–06, 2009. International Institute for Population Sciences and Macro International: Mumbai.
11. RefsumH, YajnikCS, GadkariM, et al. Hyperhomocysteinemia and elevated methylmalonic acid indicate a high prevalence of cobalamin deficiency in Asian Indians. Am J Clin Nutr. 2001; 74, 233241.
12. KatreP, BhatD, LubreeH, et al. Vitamin B12 and folic acid supplementation and plasma total homocysteine concentrations in pregnant Indian women with low B12 and high folate status. Asia Pac J Clin Nutr. 2010; 19, 335343.
13. Interna tional Institute for P opulation Sciences and ORC Macro. National Family Health Survey (NHFS-2), India 1998–99, 2001; pp. 5257. Mumbai.
14. KelleherBP, BroinSD. Microbiological assay for vitamin B12 performed in 96-well microtitre plates. J Clin Pathol. 1991; 44, 592595.
15. KelleherBP, WalsheKG, ScottJM, O'BroinSD. Microbiological assay for vitamin B12 with use of a colistin-sulfate-resistant organism. Clin Chem. 1987; 33, 5254.
16. HorneDW, PattersonD. Lactobacillus casei microbiological assay of folic acid derivatives in 96-well microtiter plates. Clin Chem. 1988; 34, 23572359.
17. TamuraT, FreebergLE, CornwellPE. Inhibition of EDTA of growth of Lactobacillus casei in the folate microbiological assay and its reversal by added manganese or iron. Clin Chem. 1990; 36, 1993.
18. PhatakP. Developmental Assessment Scale for Indian Infants, Revised Baroda Norms, 1997. University of Baroda: Baroda.
19. BayleyN. Bayley scales of infant development, 2nd edn, 1993. The Psychological Corporation: San Antonio.
20. MalinA, BharathR. Vineland Social Maturity Scale, 1992. Swayamsiddha Prakashan: Mysore.
21. World Health Organisation. WHO Child Growth Standards: length/height -for-age, weight-for-length, weight-for-height and body mass index-for-age: Methods and development, 2006. World Health Organisation: Geneva.
22. EilanderA, MuthayyaS, van der KnaapH, et al. Undernutrition, fatty acid and micronutrient status in relation to cognitive performance in Indian school children: a cross-sectional study. Br J Nutr. 2010; 103, 10561064.
23. KramerMS, McLeanFH, OlivierM, WillisDM, UsherRH. Body proportionality and head and length ‘sparing’ in growth-retarded neonates: a critical reappraisal. Pediatrics. 1989; 84, 717723.
24. JonesKM, Ramirez-ZeaM, ZuletaC, AllenLH. Prevalent vitamin B-12 deficiency in twelve-month-old Guatemalan infants is predicted by maternal B-12 deficiency and infant diet. J Nutr. 2007; 137, 13071313.
25. SchulpisK, SpiropoulosA, GavriliS, et al. Maternal – neonatal folate and vitamin B12 serum concentrations in Greeks and in Albanian immigrants. J Hum Nutr Diet. 2004; 17, 443448.
26. MuthayyaS, DwarkanathP, MhaskarM, et al. The relationship of neonatal serum vitamin B12 status with birth weight. Asia Pac J Clin Nutr. 2006; 15, 538543.
27. ChambersJC, ObeidOA, RefsumH, et al. Plasma homocysteine concentrations and risk of coronary heart disease in UK Indian Asian and European men. Lancet. 2000; 355, 523527.
28. MolloyAM, KirkePN, BrodyLC, ScottJM, MillsJL. Effects of folate and vitamin B12 deficiencies during pregnancy on fetal, infant, and child development. Food Nutr Bull. 2008; 29, S101S111.
29. KuhneT, BublR, BaumgartnerR. Maternal vegan diet causing a serious infantile neurological disorder due to vitamin B12 deficiency. Eur J Pediatr. 1991; 150, 205208.
30. StollhoffK, SchulteFJ. Vitamin B12 and brain development. Eur J Pediatr. 1987; 146, 201205.
31. SchlotzW, JonesA, PhillipsDI, et al. Lower maternal folate status in early pregnancy is associated with childhood hyperactivity and peer problems in offspring. J Child Psychol Psychiatry. 2010; 51, 594602.
32. LouwmanMW, van DusseldorpM, van de VijverFJ, et al. Signs of impaired cognitive function in adolescents with marginal cobalamin status. Am J Clin Nutr. 2000; 72, 762769.
33. AllenL, PenlandJ, BoyE, DeBaessaY, RogersL. Cognitive and neuromotor performance of Guatemalan schoolers with deficient, marginal and normal plasma B-12. FASEB J. 1999; 13, A544 Abstract.
34. MasonJB. Biomarkers of nutrient exposure and status in one-carbon (methyl) metabolism. J Nutr. 2003; 133, 941S947S.
35. BlackMM. Effects of vitamin B12 and folate deficiency on brain development in children. Food Nutr Bull. 2008; 29, S126S131.
36. CombsG. The vitamins, 3rd edn, 2008. Elsevier Academic Press: London.
37. YajnikCS, DeshpandeSS, JacksonAA, et al. Vitamin B12 and folate concentrations during pregnancy and insulin resistance in the offspring: the Pune Maternal Nutrition Study. Diabetologia. 2008; 51, 2938.
38. GodboleK, DeshmukhU, YajnikC. Nutrigenetic determinants of neural tube defects in India. Indian Pediatr. 2009; 46, 467475.
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Journal of Developmental Origins of Health and Disease
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  • EISSN: 2040-1752
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