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Anthelmintic efficacy of Achillea millifolium against gastrointestinal nematodes of sheep: in vitro and in vivo studies

Published online by Cambridge University Press:  01 September 2008

K.A. Tariq ,
M.Z. Chishti ,
F. Ahmad  and
A.S. Shawl
K.A. Tariq*
Affiliation:
Post Graduate Department of Zoology, The University of Kashmir, Srinagar-190 006, Jammu and Kashmir, India
M.Z. Chishti
Affiliation:
Post Graduate Department of Zoology, The University of Kashmir, Srinagar-190 006, Jammu and Kashmir, India
F. Ahmad
Affiliation:
Post Graduate Department of Zoology, The University of Kashmir, Srinagar-190 006, Jammu and Kashmir, India
A.S. Shawl
Affiliation:
Indian Institute of Integrative Medicine, Council of Scientific and Industrial Research, Sanatnagar, Srinagar-190 005, Jammu and Kashmir, India
*
*E-mail: drkatariq@yahoo.co.in
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Abstract

Achillea millifolium Linn., commonly called ‘Pehl-ghasa’, is used by farmers in traditional animal health care as a vermifuge. The objective of this study was to evaluate the anthelmintic efficacy of crude aqueous extracts and crude ethanolic extracts of entire A. millifolium against the gastrointestinal nematodes of sheep. The worm motility inhibition assay was used for in vitro studies and faecal egg count reduction assay was used for in vivo studies. In vitro studies revealed significant anthelmintic effects of aqueous extracts and ethanolic extracts on live Haemonchus contortus worms (P < 0.05) as evident from their paralysis and/or death at 8 h post exposure. Aqueous extracts of A. millifolium resulted in a mean worm motility inhibition of 94.44%, while ethanolic extracts resulted in mean worm motility inhibition of 88.88%. The mean mortality index of aqueous extracts was 0.95 while for ethanolic extracts it was 0.9. The lethal concentration 50 was 0.05 mg ml− 1 for aqueous extracts and 0.11 mg ml− 1 for ethanolic extracts. The in vivo anthelmintic activity of aqueous and ethanolic extracts of A. millifolium demonstrated a maximum (88.40%) nematode egg count reduction in sheep treated with aqueous extracts at 2 g kg− 1 body weight on day 15 after treatment. Ethanolic extracts resulted in a maximum of 76.53% reduction in faecal egg counts on day 15 after treatment with 2 g kg− 1 body weight. Thus, the aqueous extracts exhibited greater anthelmintic activity under both in vitro and in vivo conditions, and could be due to the presence of water-soluble active principle/s in A. millifolium. It is concluded that the entire plant of A. millifolium possesses significant anthelmintic activity and could be a potential alternative for treating cases of helminth infections in ruminants.

Type
Research Papers
Information
Journal of Helminthology , Volume 82 , Issue 3 , September 2008 , pp. 227 - 233
Copyright
Copyright © Cambridge University Press 2008

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References

Ademola, I.O., Fagbemi, B.O. & Idowu, S.O. (2005) Anthelmintic activity of extracts of Spondias mombin against gastrointestinal nematodes of sheep: studies in vitro and in vivo. Tropical Animal Health and Production 37, 223–235.CrossRefGoogle ScholarPubMed
Anita, S., Bharadwaj, P., Varshneya, C. & Telang, R.S. (2005a) Anthelmintic activity of leaves of Bauhinia variegata. Indian Veterinary Journal 82, 855–857.Google Scholar
Anita, S., Varshneya, C. & Telang, R.S. (2005b) Flowers of Tagetes patula a potential herbal anthelmintic. Indian Veterinary Journal 82, 838–840.Google Scholar
Anita, S., Varshneya, C. & Telang, R.S. (2005c) In vitro screening of Thuja orientalis leaf extract for anthelmintic activity against Haemonchus contortus. Indian Journal of Small Ruminants 11, 98–100.Google Scholar
Asha, M.K., Prashanth, D., Murali, B., Padmaja, R. & Amit, A. (2001) Anthelmintic activity of essential oil of Ocimum sanctum and eugenol. Fitoterapia 72, 669–670.CrossRefGoogle ScholarPubMed
Assis, L.M., Bevilqua, C.M.L., Morais, S.M., Vieira, L.S., Costa, C.T.C. & Souza, J.A.L. (2003) Ovicidal and larvicidal activity in vitro of Spigelia anthelmia Linn. extracts on Haemonchus contortus. Veterinary Parasitology 117, 43–49.CrossRefGoogle ScholarPubMed
Barry, T.N. & McNabb, W.C. (1999) The implications of condensed tannins on the nutritive value of temperate forages fed to ruminants. Brazilian Journal of Nutrition 81, 263–272.CrossRefGoogle ScholarPubMed
Beriajaya, , Murdiati, T.B. & Herawaty, M. (1998) Anthelmintic effect of Zingiber purpureum infusion and extract on adult worms of Haemonchus contortus in vitro. Journal Ilmu Ternak Dam Veteriner 3, 277–282.Google Scholar
Candan, F., Unlu, M., Tepe, B., Daferera, D., Polissiou, M., Sokmen, A. & Akpulat, H.A. (2003) Antioxidant and antimicrobial activity of the essential oil and methanol extracts of Achillea millifolium subsp. millifolium Afan. (Asteraceae). Journal of Ethnopharmacology 87, 215–220.CrossRefGoogle Scholar
Cavalcanti, A.M., Baggio, C.H., Freitas, C.S., Rieck, L., Sousa, R.S., Silva-santos, J.E.D., Vela, S.M. & Marques, M.C.A. (2006) Safety and anti ulcer efficacy studies of Achillea millifolium L. after chronic treatment in Wister rats. Journal of Ethnopharmacology 107, 277–284.CrossRefGoogle Scholar
Chandler, R.F., Hooper, S.N. & Harvey, M.J. (1982) Ethnobotany and phytochemistry of yarrow Achillea millifolium (Compositae). Economic Botany 36, 203–223.CrossRefGoogle Scholar
Coop, R.L. & Holmes, P.H. (1996) Nutrition and parasite interaction. International Journal for Parasitology 26, 951–962.CrossRefGoogle ScholarPubMed
Eguale, T., Tilahun, G., Debella, A., Feleke, A. & Makonnen, E. (2007a) Haemonchus contortus: in vitro and in vivo anthelmintic activity of aqueous and hydro-alcoholic extracts of Hedera helix. Experimental Parasitology 116, 340–345.CrossRefGoogle ScholarPubMed
Eguale, T., Tilahun, G., Debella, A., Feleke, A. & Makonnen, E. (2007b) In vitro and in vivo anthelmintic activity of crude extracts of Coriandrum sativum against Haemonchus contortus. Journal of Ethnopharmacology 110, 428–433.CrossRefGoogle ScholarPubMed
Ferda, C., Mehmet, U., Bekta, T., Dimitra, D., Moschos, P., Atalay, S. & Askin, A.H. (2003) Antioxidant and anti-microbial activity of the essential oil and methanol extract of Achillea millifolium subsp. millifolium Afan (Asteraceae). Journal of Ethnopharmacology 87, 215–220.Google Scholar
Githiori, J.B., Hoglund, J., Waller, P.J. & Baker, R.L. (2003) The anthelmintic efficacy of the plant, Albizia anthelmintica, against the nematode parasites Haemonchus contortus of sheep and Heligmosomoides polygyrus of mice. Veterinary Parasitology 116, 23–34.CrossRefGoogle ScholarPubMed
Githiori, J.B., Athanasiadou, S. & Thamsborg, S.M. (2006) Use of plants in novel approaches in control of gastrointestinal helminthes in live stock with emphasis on small ruminants. Veterinary Parasitology 139, 308–320.CrossRefGoogle Scholar
Innocente, G., Vegeto, E., Dall, S., Acqua, P., Ciana, M., Giorgetti, E., Agradi, A., Fico, S. & Tome, F.G. (2007) In vitro estrogenic activity of Achillea millifolium L. Phytomedicine 14, 147–152.CrossRefGoogle Scholar
Iqbal, Z., Nadeem, Q.K., Khan, M.N., Akhtar, M.S. & Waraich, F.N. (2001) In vitro anthelminitc activity of Allium sativum, Zingiber officinale, Curcurbita mexicana and Ficus religiosa. International Journal of Agriculture and Biology 3, 454–457.Google Scholar
Iqbal, Z., Lateef, M., Ashraf, M. & Jabbar, A. (2004) Anthelmintic activity of Artemisia brevifolia in sheep. Journal of Ethnopharmacology 93, 265–268.CrossRefGoogle ScholarPubMed
Iqbal, Z., Lateef, M., Jabbar, A., Mohammad, R. & Khan, M.N. (2005) Anthelmintic activity of calotropis procera (Ait) Ait F. flowers in sheep. Journal of Ethnopharmacology 102, 256–261.CrossRefGoogle ScholarPubMed
Iqbal, Z., Lateef, M., Akhter, M.S., Gayur, M.N. & Gilani, A.H. (2006a) In vivo anthelmintic activity of ginger against gastrointestinal nematodes of sheep. Journal of Ethnopharmacology 106, 285–287.CrossRefGoogle ScholarPubMed
Iqbal, Z., Lateef, M., Jabbar, A., Ghayur, M.N. & Gilani, A.H. (2006b) In vivo anthelmintic activity of Butea monosperma against trichostrongylid nematodes in sheep. Fitoterapia 77, 137–140.CrossRefGoogle ScholarPubMed
Kato, S., Bowmann, D.D. & Brown, D.L. (2000) Efficacy of Chenopodium ambrosioides as an anthelmintic for treatment of gastrointestinal nematodes in lambs. Journal of Herbs, Spices and Medicinal Plants 7, 11–25.CrossRefGoogle Scholar
Lourenco, P.M.L., Figueiredo, A.C., Barasso, J.G., Pedro, L.G., Oliveira, M.M., Deans, S.G. & Scheffer, J.J.C. (1999) Essential oils from hairy root cultures and from plant roots of Achillea millifolium. Phytochemistry 51, 637–642.CrossRefGoogle Scholar
Rabel, B., McGregor, R. & Douch, P.G.C. (1994) Improved bioassay for estimation of inhibitory effect of ovine gastrointestinal mucus and anthelmintics on nematode larval migration. International Journal of Parasitology 24, 671–676.CrossRefGoogle ScholarPubMed
Satrija, F., Retnani, E.B., Ridwan, Y. & Tiuria, R. (2001) Potential use of herbal anthelmintics as alternative antiparasitic drugs for smallholder farms in developing countries. Proceedings of the 10th Conference of the Association of Institutions of Tropical Veterinary Medicine. Copenhagen, Denmark.Google Scholar
Shawl, A.S., Srivastava, S.K., Syamasundar, K.V., Tripathi, S. & Raina, V.K. (2002) Essential oil composition of Achillea millifolium growing wild in Kashmir. Flavour and Fragrance Journal 17, 165–168.CrossRefGoogle Scholar
Soulsby, E.J.L. (1982) Helminths, arthropods and protozoa of domesticated animals. 7th edn. pp. 765–767. London, The English Language Book Society and Bailliere Tindall.Google Scholar
Stoll, N.R. (1930) On methods of counting nematode ova in sheep dung. Parasitology 22, 116.CrossRefGoogle Scholar
Ulubelen, A., Oksuz, S. & Schuster, A. (1990) Sesquiterpene lactones from Achillea millifolium subsp. millifolium. Phytochemistry 29, 3948–3949.CrossRefGoogle Scholar
Vercruysse, J. & Claerebout, E. (2001) Treatment vs. non-treatment of helminth infections in cattle: defining the threshold. Veterinary Parasitology 98, 195–214.CrossRefGoogle ScholarPubMed
Yaeesh, S., Jamal, Q., Khan, A.U. & Gilani, A.H. (2006) Studies on hepatoprotective, anti-spasmodic and calcium antagonist activities of the aqueous-methanol extract of Achillea millifolium. Phytotherapy Research 20, 546–551.CrossRefGoogle Scholar