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Studies on host specificity in Paragonimus westermani: II. Histochemical and cytochemical characterization of metacercariae and worms from rats and dogs

Published online by Cambridge University Press:  05 June 2009

Takahiro Fujino
Department of Parasitology, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan
Koichi Fukuda
Department of Parasitology, National Defence Medical College, Tokorozawa 359, Japan
Fusanori Hamajima
Department of Parasitology, National Defence Medical College, Tokorozawa 359, Japan
Yoichi Ishii
Department of Parasitology, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan


Histochemical tests were done on newly excysted metacercariae and worms recovered from an abnormal host (rat) and the definitive host (dog) for some oxidoreductases, phosphatases and glycosidases. The results demonstrate that rat worms have enzymatic distribution and intensities more similar to those of metacercariae than to adult worms from dogs. Ultracytochemical examination of acid and alkaline phosphatase and Mg-ATPase activity was also carried out. Acid phosphatase activity occurred exceptionally in the excretory bladder and caeca of dog worms. No activity was observed in rat worms except for lysosomal granules in the tegument. Alkaline phosphatase activity was exhibited in the excretory bladder in both dog and rat worms. Mg-ATPase activity occurred in the tegument and parenchymal cells in dog worms and in the excretory bladder in rat worms. In metacercariae, little or no reaction for these enzymes was present except for Mg-ATPase activity on the excretory ducts. These observations, together with the histochemical results, indicate that metabolic activity in rat worms is higher than in metacercariae although it is strongly reduced compared with dog worms.

Research Article
Copyright © Cambridge University Press 1989

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Barka, T. (1960) A simple azo-dye method for histochemical demonstration of acid phosphatase. Nature, 187, 248249.CrossRefGoogle ScholarPubMed
Barka, T. & Anderson, F. J. (1963) Histochemistry, Theory, Practice and Bibliography. Hoeber, New York, 239pp, Hoeber Med. Div. Harper and Row, Publ., New York.Google Scholar
Bogitsh, B. J. & Nunnally, D. A. (1966) Histochemistry of Hymenolepis microstoma (Cestoda: Hymenolepididae). II. Regional distribution of succinic dehydrogenase. Parasitology, 56, 5561.CrossRefGoogle ScholarPubMed
Burstone, M. S. (1960) Histochemical demonstration of cytochrome oxidase with new amine reagents. Journal of Histochemistry and Cytochemistry, 8, 6370.CrossRefGoogle ScholarPubMed
Burstone, M. S. & Keys, P. H. (1957) Study on calcification. I. The effect of inhibition of enzyme activity on developing bone and dentin. American Journal of Pathology, 33, 12291235.Google Scholar
Cheng, T. C. (1964) Studies on phosphatase system in hepatopancreatic cells of the molluscan host of Echinoparyphium sp. and in the rediae and cercariae of this trematode. Parasitology, 54, 7379.CrossRefGoogle Scholar
Coil, W. H. (1958) Alkaline phosphatase in the trematode excretory system. Proceedings of the Helminthological Society of Washington, 25, 137138.Google Scholar
Dusanic, D. G. (1959) Histochemical observations of alkaline phosphatase in Schistosoma mansoni. Journal of Infectious Diseases, 105, 18.CrossRefGoogle ScholarPubMed
Erasmus, D. A. (1967) Ultrastructural observations on the reserve bladder system of Cyathocotyle bushiensis Khan, 1962 (Trematoda: Strigeoidea) with special reference to lipid excretion. Journal of Parasitology, 53, 525536.CrossRefGoogle ScholarPubMed
Ernst, S. C. (1976) Biochemical and cytochemical studies of alkaline phosphatase activity in Schistosoma mansoni. Rice University Studies, 62, 8195.Google Scholar
Fujino, T., Fukuda, K., Hamajima, F. & Ishii, Y. (1989) Studies on host specificity in Paragonimus westermani: I. Ultrastructural characterization of metacercariae and juvenile and adult worms from abnormal and definitive hosts. Journal of Helminthology, 63, 239249.CrossRefGoogle ScholarPubMed
Fujino, T., Higo, H. & Ishii, Y. (1983a) Histochemical studies of glycosidase activity in juveniles and adults of the lung fluke Paragonimus. Parasitology, 86, 119126.CrossRefGoogle ScholarPubMed
Fujino, T., Threadgold, L.T. & Ishii, Y. (1983b) Phosphatases ultracytochemically observed in juveniles and adults of Fasciola hepatica. Japanese Journal of Parasitology, 32, 112.Google Scholar
Goldberg, E. & Nolf, L. O. (1954) Succinic dehydrogenase activity in the cestode Hymenolepis nana. Experimental Parasitology, 3, 275284.CrossRefGoogle ScholarPubMed
Gomori, G. (1952) In: Microscopic Histochemistry. Principles and Practice. University of Chicago Press, Chicago. 189pp.Google Scholar
Halton, D. W. (1967) Studies on phosphatase activity in Trematoda. Journal of Parasitology, 53, 4654.CrossRefGoogle ScholarPubMed
Hamajima, F., Fukuda, K. & Oguma, T. (1984) Morphology of the nuclei, some cytoplasmic organelles and inclusions in the body walls of Paragonimus westermani in final hosts. Japanese Journal of Parasitology, 33, 561572.Google Scholar
Hayashi, M. (1965) Histochemical demonstration of N-acetyl-β-glucosaminidase employing naphthol AS-BI N-acetyl-β-glucosaminide as substrate. Journal of Histochemistry and Cytochemistry, 13, 355360.CrossRefGoogle ScholarPubMed
Hedrick, R. M. (1956) The distribution of succinic dehydrogenase activity in Hymenolepis diminuta and Raillietina cesticillus. Journal of Parasitology, 42, (sect. 2, suppl.), 34.Google Scholar
Holt, S. J. & Withers, R. F. J. (1952) Cytochemical localization of esterases using indoxyl derivatives. Nature, 170, 10121014.CrossRefGoogle ScholarPubMed
Lojda, Z. (1970) Indigogenic methods for glycosidases. I. An improved method for β-D-glucosidase and its application to localization studies of intestinal and renal enzymes. Histochemie, 22, 347361.Google ScholarPubMed
Mayahara, H., Hirano, H., Saito, T. & Ogawa, K. (1967) The new lead citrate method for the ultracytochemical determination of activity of non-specific alkaline phosphatase (orthophosphoric monoester phosphohydrolase). Histochemie, 11, 8896.CrossRefGoogle Scholar
Miyazaki, I. (1978) On two types of the lung fluke which has been called Paragonimus westermani, with proposition of the new Japanese name. Japanese Medical Journal, 2819, 4348.Google Scholar
Moore, M. N. & Halton, D. W. (1976) Fasciola hepatica: histochemical observations on juveniles and adults and the cytopathological changes induced in infected mouse liver. Experimental Parasitology, 40, 212224.CrossRefGoogle ScholarPubMed
Nachlas, M. M., Tsou, D. E., Souza, E. De., Chang, C. S. & Seligman, A. M. (1957) Cytochemical demonstration of succinic dehydrogenase by the use of a new p-nitrophenyl substituted ditetrazole. Journal of Histochemistry and Cytochemistry, 5, 420436.CrossRefGoogle ScholarPubMed
Probert, A. J. (1966) Histochemical studies on the rediae and cercariae of Echinoparyphium recurvatum Linstow. Nature, 210, 550551.CrossRefGoogle Scholar
Roy, T. K. (1980) Distribution & functional significance of phosphatases in the bovine amphistome Ceylonocotyle scoliocoelium. Indian Journal of Experimental Biology, 18, 385392.Google ScholarPubMed
Sharma, P. N. (1978) Histochemical distribution of succinic dehydrogenase in the lymphatic system of a trematode Ceylonocotyle scoliocoelium. Journal of Helminthology, 52, 159162.CrossRefGoogle Scholar
Threadgold, L. T. (1968) Electron microscope studies of Fasciola hepatica. VI. The ultrastructural localization of phosphatases. Experimental Parasitology, 23, 264276.CrossRefGoogle ScholarPubMed
Wachstein, M. & Meisel, E. (1957) Histochemistry of hepatic phosphatases at a physiologic pH. American Journal of Clinical Pathology, 27, 1323.CrossRefGoogle Scholar