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The development of PCR methodology for the identification of species of the tapeworm Moniezia from cattle, goats and sheep in central Vietnam

Published online by Cambridge University Press:  10 November 2011

T.D. Nguyen ,
Q.D. Le ,
V.V. Huynh ,
S.T. Nguyen ,
T.V. Nguyen  and
H. Vu-Khac
T.D. Nguyen
Affiliation:
Department of Parasitology, Central Vietnam Veterinary Institute, km4 Dong De Street, Nha Trang, Vietnam
Q.D. Le
Affiliation:
Department of Parasitology, Central Vietnam Veterinary Institute, km4 Dong De Street, Nha Trang, Vietnam
V.V. Huynh
Affiliation:
Department of Parasitology, Central Vietnam Veterinary Institute, km4 Dong De Street, Nha Trang, Vietnam
S.T. Nguyen
Affiliation:
Department of Parasitology, Central Vietnam Veterinary Institute, km4 Dong De Street, Nha Trang, Vietnam
T.V. Nguyen
Affiliation:
Department of Parasitology, Central Vietnam Veterinary Institute, km4 Dong De Street, Nha Trang, Vietnam
H. Vu-Khac*
Affiliation:
Department of Biotechnology, Central Vietnam Veterinary Institute, km4 Dong De Street, Nha Trang, Vietnam
*
*Fax: 84-58-3831592 E-mail: vukhac68@hotmail.com
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Abstract

The aims of this study were to investigate the prevalence of Moniezia spp. in domestic ruminants in central Vietnam and to develop a polymerase chain reaction (PCR) technique to distinguish M. expansa from M. benedeni. Among 2040 examined domestic animals (540 cattle, 800 goats, 700 sheep) Moniezia was recovered from 5.4% of cattle, 16.4% of sheep and 20.6% of goats. A set of primers for PCR was designed to classify M. expansa and M. benedeni based on the amplification of DNA corresponding to the internal transcribed spacer of 5.8S rRNA. The 457 specimens (75 from cattle, 162 from goats, 150 from sheep, 30 from horses, 30 from chickens and 10 from dogs) were subjected to PCR for classification of Moniezia spp. PCR products with the expected sizes were amplified from bovine, ovine and caprine specimens. No specific PCR products were found for specimens from horses, chickens and dogs. Of the 75 specimens from cattle, nine were classified as M. expansa and 66 were M. benedeni. Among 162 caprine specimens, 138 were M. expansa and 24 were M. benedeni. The distribution of M. expansa and M. benedeni in 150 ovine specimens was 132 and 18, respectively. These results show that M. expansa is dominant in goats and sheep, whereas M. benedeni is more common in cattle; PCR can be used for classification of these two species.

Type
Research Papers
Information
Journal of Helminthology , Volume 86 , Issue 4 , December 2012 , pp. 426 - 429
Copyright
Copyright © Cambridge University Press 2011

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