Hostname: page-component-848d4c4894-nr4z6 Total loading time: 0 Render date: 2024-05-26T13:18:45.358Z Has data issue: false hasContentIssue false
  • English
  • Français

Echinococcus granulosus: characterization of the Spanish strains using in vitro vesicular development

Published online by Cambridge University Press:  05 June 2009

F. Ponce Gordo  and
C. Cuesta Bandera
F. Ponce Gordo
Affiliation:
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain
C. Cuesta Bandera
Affiliation:
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain
Get access Rights & Permissions [Opens in a new window]

Abstract

Differences in in vitro vesicular development (microcyst formation) in three Spanish strains of Echinococcus granulosus (sheep–cattle, horse and pig–goat) are reported. Microcyst formation occurred in 19–37 days (sheep strain), 9–18 days (pig strain) and 35–47 days (horse strain). Comparing these results with those from human samples (microcyst formation in 24–38 days), it is possible to consider the sheep strain as the most likely source of human infections in Spain.

Type
Research Papers
Information
Journal of Helminthology , Volume 71 , Issue 1 , March 1997 , pp. 61 - 67
Copyright
Copyright © Cambridge University Press 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Arme, C. (1987) Cestoda. pp. 282–317in Taylor, A.E.R. & Baker, J.R. (Eds) In vitro methods for parasite cultivation. London, Academic Press.Google Scholar
Bénex, J. (1968) Considérations expérimentales nouvelles sur l'évolution in vitro en milieu liquide des larves d'Echinococcus granulosus. Annales de Parasitologie 43, 561–572.Google Scholar
Brudnjak, Z., Cvetnic, S. & Wikerhauser, T. (1970) Cystic development of the protoscoleces and brood capsules of Echinococcus granulosus in cell cultures and cell-free media. Veterinary Archives, Zagreb 40, 292–296.Google Scholar
Casado, N. & Rodríguez-Caabeiro, F. (1988a) Cultivo in vitro de protoescólex de E. granulosus en dirección vesicular. I. Efecto del pH. Revista Ibérica de Parasitología 48, 17–23.Google Scholar
Casado, N. & Rodríguez-Caabeiro, F. (1988b) Cultivo in vitro de protoescólex de E. granulosus en dirección vesicular. II. Estudio de la idoneidad de diferentes medios. Revista Ibérica de Parasitología 48, 155–163.Google Scholar
Casado, N. & Rodríguez-Caabeiro, F. (1989) Ultrastructural study of in vitro larval development of Echinococcus granulosus protoscoleces. International Journal for Parasitology 19, 21–28.Google Scholar
Casado, N., Criado, A., Jimenez, A., De Armas, C., Brasa, C., Pérez-Serrano, J. & Rodríguez-Caabeiro, F. (1992) Viability of Echinococcus granulosus cysts in mice following cultivation in vitro. International Journal for Parasitology 22, 335–339.CrossRefGoogle Scholar
Cuesta Bandera, C. (1988) Especiación, epidemiología y control de la hidatidosis en España. Proceedings of VIII Reunión Científica Nacional de la Asociación Española de Hidatidología (Córdoba, Spain), 38–42.Google Scholar
Cuesta Bandera, C., McManus, D.P. & Rishi, A.K. (1988) Characterization of Echinococcus granulosus of Spanish origin by DNA restriction endonuclease analysis and Southern blot hybridization. International Journal for Parasitology 18, 137–141.Google Scholar
Dévé, F. (1928) Scoliciculture hydatique en sac de collodion et in vitro. Comptes Rendus Hebdomadaires de la Société de Biologie 98, 1176–1177.Google Scholar
Heath, D.D. & Lawrence, S.B. (1976) Echinococcus granulosus: development in vitro from oncosphere to immature hydatid cyst. Parasitology 73, 417–423.Google Scholar
Heath, D.D. & Osborn, P.J. (1976) Formation of Echinococcus granulosus laminated membrane in a defined medium. International Journal for Parasitology 6, 467–471.Google Scholar
Howell, M.J. (1986) Cultivation of Echinococcus species in vitro. pp. 143–163in Thompson, R.C.A. (Ed.) The biology of Echinococcus and hydatid disease. London, George Allen & Unwin.Google Scholar
Ponce Gordo, F. & Cuesta Bandera, C. (1995) Echinococcus granulosus: observations of strobilar development in in vitro monophasic culture. Journal of Helminthology 69, 173–175.Google Scholar
Rogan, M.T. & Richards, K.S. (1986) In vitro development of hydatid cysts from posterior bladders and ruptured brood capsules of equine Echinococcus granulosus. Parasitology 92, 379–390.CrossRefGoogle Scholar
Siles Lucas, M., Cuesta Bandera, C. & César Benito, M. (1993) Random amplified polymorphic DNA technique for speciation studies of Echinococcus granulosus. Parasitology Research 79, 343–345.Google Scholar
Siles Lucas, M., Felleisen, R., Cuesta Bandera, C., Gottstein, B. & Eckert, J. (1994) Comparative genetic analysis of Swiss and Spanish isolates of Echinococcus granulosus by southern hybridization and random amplified polymorphic DNA technique. Applied Parasitology 35, 107–117.Google Scholar
Siles Lucas, M., Benito, M.C. & Cuesta Bandera, C. (1996) Echinococcus granulosus: genomic and isoenzymatic study of Spanish strains isolated from different intermediate hosts. Veterinary Parasitology 63, 273–282.Google Scholar
Smyth, J.D. (1962) Studies on tapeworm physiology. X. Axenic cultivation of the hydatid organism, Echinococcus granulosus: establishment of a basic technique. Parasitology 52, 441–457.Google Scholar
Smyth, J.D. (1967) Studies on tapeworm physiology. XI. In vitro cultivation of Echinococcus granulosus from the protoscolex to the strobilate stage. Parasitology 57, 111–133.Google Scholar
Smyth, J.D. (1979) Echinococcus granulosus and Echinococcus multilocularis: in vitro culture of the strobilar stages from protoscolex. Angewandte Parasitologie 20, 137–147.Google Scholar
Smyth, J.D. (1985) In vitro culture of Echinococcus spp. Proceedings of XIII Congreso Internacional de Hidatidología (Madrid, Spain), 84–90.Google Scholar
Smyth, J.D. (1990) Cestoda. pp. 77–154in Smyth, J.D. (Ed.) In vitro cultivation of parasitic helminths. Boca Raton, Florida, CRC Press.Google Scholar
Smyth, J.D. & Davies, Z. (1974a) Occurrence of physiological strains of Echinococcus granulosus demonstrated by in vitro culture of protoscoleces from sheep and horse hydatid cysts. International Journal for Parasitology 4, 443–445.Google Scholar
Smyth, J.D. & Davies, Z. (1974b) In vitro culture of the strobilar stage of Echinococcus granulosus (sheep strain): a review of basic problems and results. International Journal for Parasitology 4, 631–644.Google Scholar
Smyth, J.D., Howkins, A.B. & Barton, M. (1966) Factors controlling the differentiation of the hydatid organism, Echinococcus granulosus, into cystic or strobilar stages in vitro. Nature 211, 1374–1377.Google Scholar
Thompson, R.C.A. (1986) Biology and systematics of Echinococcus. pp. 5–43in Thompson, R.C.A. (Ed.) The biology of Echinococcus and hydatid disease. London, George Allen & Unwin.Google Scholar
Thompson, R.C.A. (1995) Biology and systematics of Echinococcus. pp. 1–50in Thompson, R.C.A. & Lymbery, A.J. (Eds) Echinococcus and hydatid disease. Wallingford, CAB International.Google Scholar
Thompson, R.C.A. & Allsopp, C.E. (1988) Hydatidosis: veterinary perspectives and annotated bibliography. 246 pp. Wallingford, CAB International.Google Scholar
Thompson, R.C.A. & Lymbery, A.J. (1988) The nature, extent and significance of variation within the genus Echinococcus. Advances in Parasitology 27, 209–258.Google Scholar