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Factors affecting the reliability of the McMaster technique

  • Alison Dunn (a1) and Anne Keymer (a1)

Factors detraction from the reliability of faecal egg counts based on the McMaster technique include variation in flotation time (interval between loading chamber and counting eggs) and sample dilution (ratio of faecal material to salt solution). We recommend standardization of both these variables as normal laboratory procedure, and propose optima of a 30 minute flotation time and a sample dilution of 15 ml salt solution/ g faeces for use of the McMaster technique in the estimation of the fecundity of Heligmosomoides polygyrus (Nematoda).

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R. M. Anderson & G. A. Schad (1985). Hookworm burdens and faecal egg counts: an analysis of the biological basis of variation. Transactions of the Royal Society of Tropical Medicine and Hygiene. 79, 812825.

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Journal of Helminthology
  • ISSN: 0022-149X
  • EISSN: 1475-2697
  • URL: /core/journals/journal-of-helminthology
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