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Genetic diversity within the genus Trichinella as shown by cleavage fragment length polymorphism analysis

Published online by Cambridge University Press:  12 April 2024

Y.B. Rombout ,
S. Bosch ,
B. Mandjes ,
W Homan  and
J.W.B. van der Giessen
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Abstract

The genetic diversity within the genus Trichinella was studied using cleavage fragment length polymorphism (CFLP) analysis. The CFLP method generates specific fingerprints based on single nucleotide mutations. By this method the amplified intergenic regions of the 5S rRNA genes of the eight different genotypes of Trichinella were analysed. The CFLP pattern of T. spiralis was completely different compared with the sylvatic species T. britovi, T. nativa, T. nelsoni, and the genotypes Trichinella T5, Trichinella T6 and Trichinella T8. The T. pseudospiralis intergenic region can be differentiated by size from the other species of Trichinella.

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Type
Research Article
Information
Journal of Helminthology , Volume 75 , Issue 1 , January 2001 , pp. 67 - 72
Copyright
Copyright © Cambridge University Press 2001

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