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Life cycles of trematodes infecting six species of intertidal gastropods in Japan

Published online by Cambridge University Press:  22 July 2025

E. Ansai ,
H. Sekine ,
K. Munakata ,
T. Sase ,
M. Sasaki ,
M. Nitta ,
S. Suzuki ,
T. Abe ,
T. Takano  and
H. Fukumori
...Show all authors
E. Ansai
Affiliation:
Faculty of Science, https://ror.org/02hcx7n63Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan
H. Sekine
Affiliation:
Faculty of Science, https://ror.org/02hcx7n63Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan
K. Munakata
Affiliation:
Faculty of Science, https://ror.org/02hcx7n63Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan
T. Sase
Affiliation:
Faculty of Science, https://ror.org/02hcx7n63Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan
M. Sasaki
Affiliation:
Department of Veterinary Medicine, https://ror.org/02t9fsj94Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan
M. Nitta
Affiliation:
Pathology Division, Nansei Field Station, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, 422-1 Nakatsuhamaura, Minami-Ise, Watarai, Mie 516-0193, Japan
S. Suzuki
Affiliation:
Minamisanriku Nature Center, 69-2 Aza-Okita, Togura, Minamisanriku, Motoyoshi, Miyagi 986-0781, Japan
T. Abe
Affiliation:
Minamisanriku Nature Center, 69-2 Aza-Okita, Togura, Minamisanriku, Motoyoshi, Miyagi 986-0781, Japan
T. Takano
Affiliation:
Meguro Parasitological Museum, 4-1-1 Shimomeguro, Meguro, Tokyo 153-0064, Japan
H. Fukumori
Affiliation:
Research Center for Marine Biology, Graduate School of Life Sciences, Tohoku University, 9 Sakamoto, Asamushi, Aomori 039-3501, Japan
Y. Nakatsubata
Affiliation:
Faculty of Science, https://ror.org/02hcx7n63Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan
M. Suzuki
Affiliation:
Faculty of Science, https://ror.org/02hcx7n63Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan Fisheries Resources Institute, Japan Fisheries Research and Education Agency, Yokohama, Japan
T. Waki*
Affiliation:
Faculty of Science, https://ror.org/02hcx7n63Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan
*
Corresponding author: T. Waki; Email: tsukasa.waki@sci.toho-u.ac.jp
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Abstract

A variety of larvae and parthenitae of trematodes have been detected in gastropods in the intertidal zone in Japan. However, because of the difficulty associated with the morphological identification of these stages, they have rarely been identified to the species or higher taxonomic levels. In this study, trematodes of these stages were sampled from intertidal gastropods in the Japanese coastal regions and were identified to the species, genus, or family levels morphologically and molecularly to elucidate or predict their life cycles. Investigation of 17 gastropod species (682 individuals in total) from 14 localities led to the detection of trematodes in 47 individuals belonging to six snail species. The infected gastropods were morphologically identified as Nipponacmea fuscoviridis, Monodonta confusa, Trochus sacellum, Batillaria attramentaria, Littorina brevicula, and Purpuradusta gracilis. Our molecular analyses revealed that sporocysts, rediae, and metacercariae from the gastropods were divided into 14 species belonging to nine families: Philophthalmidae, Fellodistomidae, Gymnophallidae, Lepocreadiidae, Heterophyidae, Opisthorchiidae, Notocotylidae, Microphallidae, and Opecoelidae. These trematodes were thought to use fishes, octopuses, seabirds, and marine mammals as their definitive hosts. Marine organisms such as jellyfishes, crustaceans, and fishes are also thought to act as the second intermediate and paratenic hosts of few present trematode species. As for the other trematode species, DNA barcodes of trematodes from various marine organisms will also illuminate the life cycles in future.

Keywords

DNA barcodingintermediate hostlarvalife cycleparthenita

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Type
Research Paper
Information
Journal of Helminthology , Volume 99 , 2025 , e83
Copyright
© The Author(s), 2025. Published by Cambridge University Press

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