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Ligula intestinalis infection as a potential source of bias in the bioindication of endocrine disruption in the European chub Leuciscus cephalus

Published online by Cambridge University Press:  12 April 2024

M. Schabuss ,
M. Gemeiner ,
A. Gleiß ,
J.W. Lewis ,
I Miller ,
E. Möstl ,
U. Schober ,
W. Tschulenk ,
I. Walter  and
B. Grillitsch
M. Schabuss*
Affiliation:
Institute for Aquatic Ecotoxicology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A- 1210 Vienna, Austria: Institute of Ecology and Conservation Biology, University of Vienna, Althanstrasse 14, A- 1090 Vienna, Austria
M. Gemeiner
Affiliation:
Institute for Medical Chemistry, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A- 1210 Vienna, Austria
A. Gleiß
Affiliation:
Institute for Aquatic Ecotoxicology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A- 1210 Vienna, Austria: Institute of Medical Statistics, University of Vienna, Schwarzspanierstrasse 17, A- 1090 Vienna, Austria
J.W. Lewis
Affiliation:
School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, TW20 0EX, UK
I Miller
Affiliation:
Institute for Medical Chemistry, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A- 1210 Vienna, Austria
E. Möstl
Affiliation:
Institute for Biochemistry, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A- 1210 Vienna, Austria
U. Schober
Affiliation:
Institute for Aquatic Ecotoxicology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A- 1210 Vienna, Austria:
W. Tschulenk
Affiliation:
Institute for Histology and Embryology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A- 1210 Vienna, Austria
I. Walter
Affiliation:
Institute for Histology and Embryology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A- 1210 Vienna, Austria
B. Grillitsch
Affiliation:
Institute for Aquatic Ecotoxicology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A- 1210 Vienna, Austria:
*
*Fax: ++43 1 250774690 Email: Michael.Schabuss@vu-wien.ac.at
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Abstract

European chub Leuciscus cephalus collected from five localities in the lowland and subalpine regions of Austria were analysed for oestrogenic effects of endocrine-disrupting chemicals and the presence of the plerocercoid of the tapeworm Ligula intestinalis. Of 1494 chub analysed, only seven (six males, one female) were found to be infected with single, but large plerocercoids up to 15 cm in length. Ligula-infected fish showed comparatively immature gonads, as demonstrated by the gonadosomatic index and gamete developmental stages. Plasma levels of the egg precursor protein vitellogenin also showed concentrations ranging below the detection limit. The present results indicate that chub infected with L. intestinalis and exposed to exogenous oestrogenic compounds can result in reduced gonadal maturation and produce false oestrogen-positive diagnoses in male fish. For plasma vitellogenin levels, L. intestinalis infections can result in false oestrogen-negative diagnoses in male and female fish.

Type
Research Article
Information
Journal of Helminthology , Volume 79 , Issue 1 , March 2005 , pp. 91 - 94
Copyright
Copyright © Cambridge University Press 2005

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References

Andres, S., Ribeyre, F., Tourencq, J.-N. & Boudou, A. (2000) Interspecific comparison of cadmium and zinc contamination in the organs of four fish species along a polymetallic pollution gradient (Lot River, France). Science of the Total Environment 248, 11–25.CrossRefGoogle ScholarPubMed
Arme, C. (2002) Ligula intestinalis a tapeworm contraceptive. Biologist 49, 265–269.Google ScholarPubMed
Blüm, V., Casado, J., Lehmann, J. & Mehring, E. (1988) Farbatlas der Histologie der Regenbogenforelle. Landesanstalt für Fischerei Nordrhein-Westfalen, pp. III–VII. Berlin, Springer Verlag.CrossRefGoogle Scholar
Brion, E., Rogerieux, F., Noury, P., Migeon, B., Flammarion, P., Thybaud, E. & Porcher, J.M. (2000) Two-step purification method of vitellogenin from three teleost fish species: rainbow trout (Oncorhynchus mykiss), gudgeon (Gobio gobio) and chub (Leuciscus cephalus). Journal of Chromatography B 737, 3–12.CrossRefGoogle ScholarPubMed
Flammarion, P., Brion, F., Babut, M., Garric, J., Migeon, B., Noury, P., Thybaud, E., Tyler, C.R., Palazzi, X. (2000) Induction of fish vitellogenin and alterations in testicular structure: preliminary results of estrogenic effects in chub (Leuciscus cephalus). Ecotoxicology 9, 127–135.CrossRefGoogle Scholar
Flammarion, P., Devaux, A., Nehls, S., Migeon, B., Noury, P. & Garric, J. (2002) Multibiomarker responses in fish from the Moselle River (France). Ecotoxicology and Environmental Safety 51, 145–153.CrossRefGoogle ScholarPubMed
Grillitsch, B., Gemeiner, M., Gleiß, A., Kuchar, A., Leitner, M., Miller, I., Möstl, E., Schabuss, M., Schober, U., Tschulenk, W. & Walter, I. (2003) Bioindikation. Untersuchungen an Fischen. pp. II.1–II.132. in Hormonwirksame Stoffe in Österreichs Gewässern–ein Risiko? Vienna, Austria, Federal Environmental Agency.Google Scholar
Hecker, M. (2001) Natural variability of endocrine functions and their modulation by anthropogenic influences. Investigations of the bream (Abramis brama L.) along the river Elbe and at reference sites. Berichte aus dem Zentrum für Meeres und Klimaforschung Reihe E: Hydrobiologie und Fischereiwissenschaft 16, 54–60.Google Scholar
IUCN (1996) Red list of threatened species. Cambridge: The World Conservation Union, International Union for Conservation and Natural Resources.Google Scholar
Loot, G., Brosse, S., Lek, S. & Guegan, J.F. (2001) Behaviour of roach (Rutilus rutilus, L.) altered by Ligula intestinalis (Cestoda: Pseudophyllidea): a field demonstration. Freshwater Biology 46, 1219–1227.CrossRefGoogle Scholar
Machala, M., Dusek, L., Hilscherova, K., Kubinova, R., Juradja, P., Neca, J., Ulrich, R., Gelnar, M., Studnickova, Z. & Holoubek, I. (2001) Determination and multivariate statistical analysis of biochemical responses to environmental contaminants in feral freshwater fish Leuciscus cephalus L. Environmental Toxicology and Chemistry 20, 1141–1148.Google Scholar
Minier, C., Caltot, G., Leboulanger, F. & Hill, E.M. (2000) An investigation of the incidence of intersex fish in Seine-Maritime and Sussex regions. Analusis 28, 801–806.CrossRefGoogle Scholar
Museth, J. (2001) Effects of Ligula intestinalis on habitat use, predation risk and catchability in European minnows. Journal of Fish Biology 59, 1070–1080.CrossRefGoogle Scholar
Rydlo, M. (1994) Parasitologische Untersuchung von Aiteln Leuciscus cephalus aus Fließgewässern und Seen Österreichs. European Association of Fish Pathologists: Tagung der Deutschen Sektion der European Association of Fish Pathologists, Wolfegg/Baden Württemberg, 14./15. September 1994, 79–80.Google Scholar