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Molecular genotyping of Echinococcus granulosus using formalin-fixed paraffin-embedded preparations from human isolates in unusual tissue sites

Published online by Cambridge University Press:  20 July 2015

A. Hizem ,
S. M'rad ,
M. Oudni-M'rad ,
S. Mestiri ,
F. Hammedi ,
H. Mezhoud ,
A. Zakhama ,
M. Mokni  and
H. Babba
A. Hizem
Affiliation:
LP3M: Laboratoire de Parasitologie-Mycologie Médicale et Moléculaire, LR 12ES08, Département de Biologie Clinique B, Faculté de Pharmacie de Monastir, Université de Monastir, Monastir 5000, Tunisie
S. M'rad*
Affiliation:
LP3M: Laboratoire de Parasitologie-Mycologie Médicale et Moléculaire, LR 12ES08, Département de Biologie Clinique B, Faculté de Pharmacie de Monastir, Université de Monastir, Monastir 5000, Tunisie
M. Oudni-M'rad
Affiliation:
LP3M: Laboratoire de Parasitologie-Mycologie Médicale et Moléculaire, LR 12ES08, Département de Biologie Clinique B, Faculté de Pharmacie de Monastir, Université de Monastir, Monastir 5000, Tunisie
S. Mestiri
Affiliation:
Laboratoire d'Anatomie et Cytologie Pathologiques, EPS F. Hached, Sousse 4000, Tunisie
F. Hammedi
Affiliation:
Laboratoire d'Anatomie et Cytologie Pathologiques, EPS F. Bourguiba, Monastir 5000, Tunisie
H. Mezhoud
Affiliation:
LP3M: Laboratoire de Parasitologie-Mycologie Médicale et Moléculaire, LR 12ES08, Département de Biologie Clinique B, Faculté de Pharmacie de Monastir, Université de Monastir, Monastir 5000, Tunisie
A. Zakhama
Affiliation:
Laboratoire d'Anatomie et Cytologie Pathologiques, EPS F. Bourguiba, Monastir 5000, Tunisie
M. Mokni
Affiliation:
Laboratoire d'Anatomie et Cytologie Pathologiques, EPS F. Hached, Sousse 4000, Tunisie
H. Babba
Affiliation:
LP3M: Laboratoire de Parasitologie-Mycologie Médicale et Moléculaire, LR 12ES08, Département de Biologie Clinique B, Faculté de Pharmacie de Monastir, Université de Monastir, Monastir 5000, Tunisie Laboratoire B, Centre de maternité EPS F. Bourguiba, Monastir 5000, Tunisie
*
*Fax: 00 216 73 461 830E-mail: selim.mrad@gnet.tn
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Abstract

Cystic echinococcosis (CE) caused by Echinococcus granulosus remains a serious problem worldwide for issues relating to public health and the economy. The most predominantly affected sites are the liver and the lungs, but other organs such as the heart, the spleen and the peritoneum can also be infected. Access to cysts from uncommon sites has limited genomic and molecular investigations. In the present study, genotypes of E. granulosus sensu lato were identified from formalin-fixed paraffin-embedded tissues (FF-PETs) implicated in human CE. Tissue samples were obtained from 57 patients with histologically confirmed CE. DNA samples were analysed using Egss 1 polymerase chain reaction (PCR) specific to the mitochondrial 12S rRNA gene of E. granulosus sensu stricto. All cysts were typed as E. granulosus sensu stricto with up to 35% of the liver and 16.6% of lungs being the most frequently infected, and up to 48.4% of samples being from rare sites. No correlation was found between cyst site and either the gender or the age of patients. This study demonstrates the possibility of exploiting atypical cysts using FF-PET samples and highlights the predominance of E. granulosus sensu stricto species in the Tunisian population, even in unusual infection sites.

Information

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 4 , July 2016 , pp. 417 - 421
Copyright
Copyright © Cambridge University Press 2015 

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