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Morphological and molecular analysis of metacercariae of Diphtherostomum brusinae (Stossich, 1888) Stossich, 1903 from a new bivalve host Mytilus galloprovincialis

Published online by Cambridge University Press:  12 August 2010

C.J. Francisco ,
A. Almeida ,
A.M. Castro ,
S. Pina ,
F. Russell-Pinto ,
P. Rodrigues  and
M.J. Santos
C.J. Francisco*
Affiliation:
Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, FC4, Rua do Campo Alegre s/n, 4169-007Porto, Portugal CIMAR Laboratório Associado/CIIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental, Rua dos Bragas, 289, 4050-123Porto, Portugal
A. Almeida
Affiliation:
Departamento de Doenças Infecciosas, Instituto Nacional de Saúde Dr Ricardo Jorge, Rua Alexandre Herculano, 321, 4000-055Porto, Portugal Instituto de Ciências Biomédicas de Abel Salazar, Largo Prof. Abel Salazar, 2, 4099-003Porto, Portugal
A.M. Castro
Affiliation:
Departamento de Doenças Infecciosas, Instituto Nacional de Saúde Dr Ricardo Jorge, Rua Alexandre Herculano, 321, 4000-055Porto, Portugal
S. Pina
Affiliation:
Instituto de Ciências Biomédicas de Abel Salazar, Largo Prof. Abel Salazar, 2, 4099-003Porto, Portugal
F. Russell-Pinto
Affiliation:
CIMAR Laboratório Associado/CIIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental, Rua dos Bragas, 289, 4050-123Porto, Portugal Instituto de Ciências Biomédicas de Abel Salazar, Largo Prof. Abel Salazar, 2, 4099-003Porto, Portugal
P. Rodrigues
Affiliation:
Instituto de Ciências Biomédicas de Abel Salazar, Largo Prof. Abel Salazar, 2, 4099-003Porto, Portugal
M.J. Santos
Affiliation:
Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, FC4, Rua do Campo Alegre s/n, 4169-007Porto, Portugal CIMAR Laboratório Associado/CIIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental, Rua dos Bragas, 289, 4050-123Porto, Portugal
*
*E-mail: clairejulianaf@yahoo.com.br
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Abstract

The digenean trematode Diphtherostomum brusinae (Stossich, 1888) Stossich, 1903 presents a complex life cycle that may involve more than one intermediate host. The present study represents the first description of the metacercariae from D. brusinae infecting the labial palps of a new intermediate host, Mytilus galloprovincialis, in the Aveiro estuary, Portugal. The morphology of this parasitic stage was studied by light (LM) and scanning electron microscopy (SEM) and some differences were reported (body and sucker sizes, and spine distribution and shape). In this work, the 18S partial region of the ribosomal DNA was sequenced from D. brusinae metacercariae isolated from M. galloprovincialis collected in different localities of the Aveiro estuary. In addition, sequences from the same region of the 18S rDNA were obtained from D. brusinae cercariae and metacercariae, hosted by Nassarius reticulatus and Cerastoderma edule, respectively. No intraspecific polymorphism was detected in the 18S partial region, since there was 100% homology among all the sequences analysed. The same comparison was made for the ITS1, and we observed intraspecific polymorphism in this region. To our knowledge, this is the first report of D. brusinae metacercariae infecting the mussel M. galloprovincialis with support from morphological and molecular data.

Type
Regular research papers
Information
Journal of Helminthology , Volume 85 , Issue 2 , June 2011 , pp. 179 - 184
Copyright
Copyright © Cambridge University Press 2010

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