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The number of Oesophagostomum spp. larvae recovered in coprocultures varies with media used

Published online by Cambridge University Press:  10 July 2023

Alexander D. Hernandez*
Affiliation:
Department of Biology Kutztown University of Pennsylvania Kutztown, Pennsylvania 19530 USA
Billie Kantner
Affiliation:
Department of Biology Kutztown University of Pennsylvania Kutztown, Pennsylvania 19530 USA
Emely Santos
Affiliation:
Department of Biology Kutztown University of Pennsylvania Kutztown, Pennsylvania 19530 USA
Sara Major
Affiliation:
Rodale Institute Kutztown, Pennsylvania 19530 USA
Rick Carr
Affiliation:
Rodale Institute Kutztown, Pennsylvania 19530 USA
Yuzhi Li
Affiliation:
West Central Research and Outreach Center University of Minnesota at Morris Morris, Minnesota 10001 USA
*
Corresponding author: Alexander D. Hernandez; Email: ahernandez@kutztown.edu

Abstract

Nodular roundworms (Oesophagostomum spp.) are frequent parasites of the large intestine in several mammal species including humans and pigs, and their study often requires the use of infective larvae produced using several coproculture techniques. However, there is no published comparison of techniques to determine which yields the highest number of larvae. This study compares the number of larvae recovered from coprocultures made with charcoal, sawdust, vermiculite, and water in an experiment repeated twice using feces from a sow naturally infected with Oesophagostomum spp. at an organic farm. A higher number of larvae were recovered from coprocultures using sawdust relative to other types of media used, and this was consistent across the two trials. The use of sawdust to culture Oesophagostomum spp. larvae is rarely reported and our study suggests it can yield higher numbers relative to other media.

Type
Short Communication
Copyright
© The Author(s), 2023. Published by Cambridge University Press

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