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Polymorphism in the Dirofilaria immitis immunodominant antigen gene

Published online by Cambridge University Press:  11 April 2024

K. Sugane ,
K. Nakayama  and
H. Kato
K. Sugane*
Affiliation:
Department of Parasitology
K. Nakayama
Affiliation:
Department of Anatomy, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto City, Nagano Prefecture 390, Japan
H. Kato
Affiliation:
Department of Bioscience Fukui Prefecture, University School of Bioscience, Fukui, Japan
*
*Fax: +81 263 37 3092 Note: The nucleotide sequence data reported in this paper are available in the DDBJ, EMBL and GenBank data bases under the accession numbers AB004252 and AB004253.
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Abstract

Dg2, a gene encoding a 34 kDa immunodominant antigen of Dirofilaria immitis was cloned and demonstrated to be specifically expressed in the larval stage. In this study, a newly constructed genomic DNA library was screened by hybridization with Dg2. One of the resulting positive clones was similar to Dg2 in the structure of its exonic regions but different in number, position, size and sequence of introns. This was designated DgK. Full-length cDNA was isolated using the rapid amplification of cDNA ends (RACE) method to study the transcript corresponding to DgK. Sequence analysis revealed that the mRNA corresponding to DgK is trans-spliced during post-transcriptional processing because the 5′ end of the amplified cDNA contains seven nucleotides of the nematode-spliced leader (SL) sequence.

Type
Research Article
Information
Journal of Helminthology , Volume 73 , Issue 3 , March 1999 , pp. 265 - 272
Copyright
Copyright © Cambridge University Press 1999

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