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The protective capacity of UV-exposed Clinostomum complanatum metacercariae against challenge infections in Ardeola ibis ibis

Published online by Cambridge University Press:  13 May 2009

G. Allam  and
S.M. Aboel Hadid
G. Allam*
Affiliation:
College of Medicine and Medical Sciences, Taif University, Taif, Saudi Arabia Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt
S.M. Aboel Hadid
Affiliation:
Parasitology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt
*
*Fax: 00966-27250528 E-mail: gm_allam@yahoo.com
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Abstract

Exposure of encysted metacercariae of Clinostomum complanatum to UV light (254 nm) for 60 min reduced their development into adult worms in buff-backed herons (95.7% reduction in worm burden). Metacercariae that succeeded in developing into adult worms, showed low fecundity levels. Furthermore, 30% of eggs laid showed abnormal shape; however, all normal and abnormal eggs failed to hatch into miracidia. The effectiveness of UV-irradiated metacercariae as a vaccine was investigated. Compared to control unvaccinated herons, the vaccinated group showed a significantly high protection rate (73.8%) against challenge. In vitro, worm development after challenge showed decreased fecundity and increased egg abnormalities, where only 1.5% of all eggs produced hatched into miracidia. A passive haemagglutination test revealed increased antibody titres against soluble adult worm antigen in both vaccinated and vaccinated-challenged birds. It was concluded that vaccination of herons using encysted metacercariae UV-irradiated for 60 min can protect them against challenge infection.

Type
Research Papers
Information
Journal of Helminthology , Volume 83 , Issue 4 , December 2009 , pp. 361 - 367
Copyright
Copyright © Cambridge University Press 2009

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