Hostname: page-component-848d4c4894-m9kch Total loading time: 0 Render date: 2024-05-20T12:39:40.992Z Has data issue: false hasContentIssue false
  • English
  • Français

Studies of the effect of contralateral versus ipselateral challenge of rats primed on one side with Strongyloides ratti

Published online by Cambridge University Press:  05 June 2009

P. A. G. Wilson
P. A. G. Wilson
Affiliation:
University of Edinburgh, Department of Zoology, West Mains Road, Edinburgh, EH9 3JT, Scotland
Get access Rights & Permissions [Opens in a new window]

Abstract

The initial pathway of skin penetrating larvae of Strongyloides ratti inside the host is not systemic and could well involve local components of the lymphatic system. The experiments described were an attempt to detect an effect on immunity depending on whether larvae of a challenge infection were committed to a pathway through heavily primed or lightly primed lymph nodes. Female rats were immunised by subcutaneous injection into the right forearm of 1000 heat killed, or 1000 live, third stage larvae of S. ratti. Animals given living parasites were placed on a diet containing 0·1 or 0·2% thiabendazole 48h or 36h after ifection. Challenge infections of <100 larvae (‘exact’ doses) were applied on day 21 to either the right or left flank and, similarly, to controls that had received the anthelmintic but not the priming infection. Heat killed parasites elicited no response at all. Priming with live larvae stimulated a significant immunity (16% and 37% depression in 2 experiments), but there was no difference between rats whose challenge was on the same side as the priming dose and those which had the contralateral treatment. The significance of these results to theories of pathfinding in general, and to practical immunology, is discussed.

Keywords

Strongyloides rattiratchallenge infections
Type
Research Papers
Information
Journal of Helminthology , Volume 61 , Issue 2 , June 1987 , pp. 173 - 178
Copyright
Copyright © Cambridge University Press 1987

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bell, R. G., Adams, L. S. & Gerb, J. (1981) Strongyloides ratti: Dissociation of the rat's protective immunity into systemic and intestinal components. Experimental Parasitology, 52, 386–395.Google Scholar
Looss, A. (1905) Wanderung der Ancylostomum-und Strongyloides-Larven von der Haut nach dem Darm. Comptes Rendus de 6e Congres internationale de Zoologie, Berne, 1904, 225–233.Google Scholar
Love, R. J., Kelly, J. D. & Dineen, J. K. (1974) Nippostrongylus brasiliensis: Effects of immunity on pre-intestinal and intestinal larval stages of the parasite. International Journal for Parasitology, 4, 183–191.Google Scholar
Moqbel, R. (1980) Histopathological changes following primary, secondary and repeated infections of rats with Strongyloides ratti, with special reference to tissue eosinophils. Parasite Immunology, 2, 11–17.Google Scholar
Murrell, K. D. (1980) Strongyloides ratti: Acquired resistance in the rat to preintestinal migrating larvae. Experimental Parasitology, 50, 417–425.Google Scholar
Murrell, K. D. (1981) Protective role of immunoglobulin G in immunity to Strongyloides ratti. Journal of Parasitology, 67, 167–73.Google Scholar
Ponzio, N. M., Baine, Y. & Thorbecke, G. J. (1980) Localization of functional memory B cells at sites of antigen localization and its relationship to local aspects of immunological memory. In: Phylogeny of Immunological Memory (editor Manning, M. J.) Elsevier/North Holland Biomedical Press: Amsterdam. pp291–307.Google Scholar
Taliaferro, W. H. & Sarles, M. P. (1939) The cellular reaction in the skin, lungs and intestine of normal and immune rats after infection with Nippostrongylus muris. Journal of Infectious Diseases, 64, 157–192.Google Scholar
Tilney, N. L. (1971) Patterns of lymphatic drainage in the adult laboratory rat. Journal of Anatomy, 109, 369–383.Google Scholar
Wilson, P. A. G. (1979) Tracking radioactive larvae of Strongyloides ratti in the host. Parasitology, 79, 29–38.Google Scholar
Wilson, P. A. G. (1983) Roundowrm juvenile migration in mammals: the pathways of skinpenetrators reconsidered. In Aspects of Parasitology — A Festschift dedicated to the 50th anniversary of the Institute of Parasitology, McGill University, 1932–1982 (editor Meerovitch, E.): Institute of Parasitology: Montreal, pp. 459–485.Google Scholar
Wilson, P. A. G., Cameron, M. & Scott, D. S. (1978) Strongyloides ratti in virgin female rats: studies of oestrous cycle effects and general variability. Parasitology, 76, 221–227.Google Scholar
Wilson, P. A. G. & Simpson, N. E. (1981) Dynamics of infection in rats given low doses of homogonic and heterogonic Strongyloides ratti. Parasitology, 83, 459–475.Google Scholar
Wilson, P. A. G. & Simpson, N. E. (1982) Strongyloides ratti: studies of 75Se labelled larvae of the bomogonic strain in female hosts. Parasitology, 84, 443–454.Google Scholar
Wilson, P. A. G., Simpson, N. E. & Seaton, D. S. (1986) The effect if method of infection on the pathway of juvenile Strongyloides ratti in the host. Journal of Helminthology, 60, 79–91.Google Scholar
Wilson, P. A. G., Steven, G. E. & Simpson, N. E. (1982) Strongyloides ratti (homogonic): the time-course of early migration in the generalized host deduced from emperiments in lactating rats. Parasitology, 85, 533–542.Google Scholar