Skip to main content
×
×
Home

Antioxidant effect of pomegranate extract in reducing acute inflammation due to myringotomy

  • V Kahya (a1), A Meric (a1), M Yazici (a2), M Yuksel (a3), A Midi (a4) and O Gedikli (a5)...
Abstract
Objective:

To assess the effect of pomegranate extract on acute inflammation due to myringotomy.

Design:

Prospective, randomised study.

Subjects:

Thirty Sprague–Dawley rats were divided into three groups. Group one constituted controls. Group two underwent myringotomy. Group three underwent myringotomy and also received 100 µl/day pomegranate extract, via gavage, one day before and two days after surgery. Following sacrifice 48 hours after myringotomy, the animals' right ears were used to determine the concentration of reactive oxygen species, using the chemiluminescence method; left ears were used for histological study.

Results:

Reactive oxygen species levels were significantly decreased in group three compared with group two (p < 0.01). The density of inflammatory cells in group three was significantly less than that in group two (p < 0.01). Lamina propria thickness and vessel density were also significantly decreased in group three compared with group two (p < 0.01).

Conclusion:

Our results indicate that oral pomegranate extract decreases reactive oxygen species concentration and acute inflammation in the tympanic membrane after myringotomy.

Copyright
Corresponding author
Address for correspondence: Dr Aysenur Meric, Atakoy 9, Kisim A3A Blok D:36, Bakirkoy, Istanbul, Turkey Fax: +90 212 6217580 E-mail: aysenurmeric@yahoo.com
References
Hide All
1Mattsson, C, Magnuson, K, Hellström, S. Myringotomy: a prerequisite for the development of myringosclerosis. Laryngoscope 1998;108:102–6
2Riley, DN, Herberger, S, McBride, G. Myringotomy and ventilation tube insertion: a ten year follow up. J Laryngol Otol 1997;111:257–61
3Mattsson, C, Magnuson, K, Hellström, S. Myringosclerosis caused by increased oxygen concentration in traumatized tympanic membranes. Experimental study. Ann Otol Rhinol Laryngol 1995;104:625–32
4Mattsson, C, Johansson, C, Hellström, S. Myringosclerosis develops within 9 hours of myringotomy. ORL J Otorhinolarygol Relat Spec 1999;61:31–6
5Mattsson, C, Marklund, SL, Hellström, S. Application of oxygen free radical scavengers to diminish the occurrence of myringosclerosis. Ann Otol Rhinol Laryngol 1997;106:513–18
6Polat, S, Oztürk, O, Uneri, C, Yüksel, M, Haklar, G, Bozkurt, S. Determination of reactive oxygen species in myringotomized tympanic membranes: effect of vitamin E treatment. Laryngoscope 2004;114:720–5
7Spratley, JE, Hellström, SO, Mattsson, CK, Pais-Clemente, . Topical ascorbic acid reduces myringosclerosis in perforated tympanic membranes. Ann Otol Rhinol Laryngol 2001;110:585–91
8Akbaş, Y, Pata, YS, Görür, K, Polat, G, Polat, A, Ozcan, C. The effect of L-carnitine on the prevention of experimentally induced myringosclerosis in rats. Hear Res 2003;184:107–12
9Ozcan, C, Gorur, K, Cinel, L, Talas, DU, Unal, M, Cinel, . The inhibitory effect of topical N- acetylcysteine application on myringosclerosis in perforated rat tympanic membrane. Int J Pediatr Otorhinolaryngol 2002;63:179–84
10Esmaillzadeh, A, Tahbaz, F, Gaieni, I. Cholesterol-lowering effect of concentrated pomegranate juice consumption in type II diabetic patients with hyperlipidemia. Int J Vitam Nutr Res 2006;76:147–51
11de Nigris, F, Williams-Ignarro, S, Sica, V, Lerman, LO, D'Armiento, FP, Byrns, RE et al. Effects of a pomegranate fruit extract rich in punicalagin on oxidation-sensitive genes and eNOS activity at sites of perturbed shear stress and atherogenesis. Cardiovasc Res 2007;73:414–23
12Tugcu, V, Kemahli, E, Ozbek, E, Arinci, YV, Uhri, M, Erturkuner, P et al. Protective effect of a potent antioxidant, pomegranate juice, in the kidney of rats with nephrolithiasis induced by ethylene glycol. J Endourol 2008;22:2723–31
13Aviram, M, Dornfeld, L. Pomegranate juice consumption inhibits serum angiotensin converting enzyme activity and reduces systolic blood pressure. Atherosclerosis 2001;158:195–8
14Kaplan, M, Hayek, T, Raz, A, Coleman, R, Dornfeld, L, Vaya, J et al. Pomegranate juice supplementation to atherosclerotic mice reduces macrophage lipid peroxidation, cellular cholesterol accumulation and development of atherosclerosis. J Nutr 2001;131:2082–9
15Sade, J, Luntz, M. Dynamic measurements of gas composition in the middle ear. II: steady state value. Acta Otolaryngol 1993;113:353–7
16Parks, RR, Huang, C-C, Haddad, J. Evidence of oxygen radical injury in experimental otitis media. Laryngoscope 1994;104:1389–92
17Mattsson, C, Hellström, S. Inhibition of the development of myringosclerosis by local administration of fenspiride, an anti-inflammatory drug. Eur Arch Otorhinolaryngol 1997;254:425–9
18Aviram, M, Dornfeld, L, Kaplan, M, Coleman, R, Gaitini, D, Nitecki, S et al. Pomegranate juice flavonoids inhibit low-density lipoprotein oxidation and cardiovascular diseases: studies in atherosclerotic mice and in humans. Drugs Exp Clin Res 2002;28:4962
19Tsuda, T, Horio, F, Osawa, T. Cyanidin 3-O-beta-D-glucoside suppresses nitric oxide production during a zymosan treatment in rats. J Nutr Sci Vitaminol (Tokyo) 2002;48:305–10
20Görür, K, Ozcan, C, Polat, A, Unal, M, Tamer, L, Cinel, I et al. The anti-oxidant and anti-apoptotic activities of selenium in the prevention of myringosclerosis in rats. J Laryngol Otol 2002;116:426–9
21Forséni, M, Bagger-Sjöbäck, D, Hultcrantz, M. A study of inflammatory mediators in the human tympanosclerotic middle ear. Arch Otolaryngol Head Neck Surg 2001;127:559–64
22Kaptan, ZK, Emir, H, Gocmen, H, Uzunkulaoglu, H, Karakas, A, Senes, M et al. Ginkgo biloba, a free oxygen radical scavenger, affects inflammatory mediators to diminish the occurrence of experimental myringosclerosis. Acta Otolaryngol 2008;17:16
23Tahar Aissa, J, Hultcrantz, M. Acute tympanic membrane perforations and the early immunological response in rats. Acta Otolaryngol 2009;23:16
24Schiff, M, Poliquin, JF, Cantanzaro, A. Tympanosclerosis: a theory of pathogenesis. Ann Otol Rhinol Laryngol Suppl 1980;89:1628
25Hellström, S, Spratley, J, Eriksson, PO, Pais-Clemente, M. Tympanic membrane vessel revisited: a study in an animal model. Otol Neurotol 2003;24:494–9
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

The Journal of Laryngology & Otology
  • ISSN: 0022-2151
  • EISSN: 1748-5460
  • URL: /core/journals/journal-of-laryngology-and-otology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords

Metrics

Full text views

Total number of HTML views: 2
Total number of PDF views: 18 *
Loading metrics...

Abstract views

Total abstract views: 148 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 15th August 2018. This data will be updated every 24 hours.