Skip to main content Accessibility help

The effect of intratympanic oxytocin treatment on rats exposed to acoustic trauma

  • F C Akin Ocal (a1), G G Kesici (a2), S G Gurgen (a3), R Ocal (a2) and S Erbek (a4)...



To investigate whether oxytocin can prevent ototoxicity related to acoustic trauma.


Twenty-eight rats were divided into four groups: noise (group 1), control (group 2), noise plus oxytocin (group 3), and oxytocin (group 4). Intratympanic oxytocin was administered on days 1, 2, 4, 6, 8 and 10 in groups 3 and 4. Groups 1 and 3 were exposed to acoustic trauma. Distortion product otoacoustic emission and auditory brainstem response testing were performed in all groups.


In group 1, auditory brainstem response thresholds increased significantly after acoustic trauma. In group 3, auditory brainstem response thresholds increased significantly on day 1 after acoustic trauma, but there were no significant differences between thresholds at baseline and on the 7th and 21st days. In group 1, significant differences were observed between distortion product otoacoustic emission signal-to-noise ratios measured before and on days 1, 7 and 21 after acoustic trauma. In group 3, no significant differences were observed between the distortion product otoacoustic emission signal-to-noise ratios measured before and on days 7 and 21 after acoustic trauma.


Oxytocin had a therapeutic effect on rats exposed to acoustic trauma in this experiment.


Corresponding author

Author for correspondence: Dr F Ceyda Akin Ocal, Otorhinolaryngology Clinic, Gulhane Training and Research Hospital, 38000 Ankara, Turkey E-mail:


Hide All

Dr F C Akin Ocal takes responsibility for the integrity of the content of the paper

Presented at the ENT World Congress, 24–28 June 2017, Paris, France.



Hide All
1Lim, HW, Choi, SH, Kang, HH, Ahn, JH, Chung, JW. Apoptotic pattern of cochlear outer hair cells and frequency-specific hearing threshold shift in noise-exposed BALB/c mice. Clin Exp Otorhinolaryngol 2008;1:80–5
2Bohne, BA, Harding, GW. Degeneration in the cochlea after noise damage: primary versus secondary events. Am J Otol 2000;21:505–9
3Mukherjea, D, Ghosh, S, Bhatta, P, Steth, S, Tupal, S, Borse, V et al. Early investigational drugs for hearing loss. Expert Opin Investig Drugs 2015;24:201–17
4Ohlemiller, KK, McFadden, SL, Ding, DL, Lear, PM, Ho, YS. Targeted mutation of the gene for cellular glutathione peroxidase (Gpx1) increases noise-induced hearing loss in mice. J Assoc Res Otolaryngol 2000;1:243–54
5Ohlemiller, KK, McFadden, SL, Ding, DL, Flood, DG, Reaume, AG, Hoffman, EK et al. Targeted deletion of the cytosolic Cu/Zn-superoxide dismutase gene (Sod1) increases susceptibility to noise-induced hearing loss. Audiol Neurootol 1999;4:237–46
6Le Prell, CG, Yamashita, D, Minami, SB, Yamasoba, T, Miller, JM. Mechanisms of noise-induced hearing loss indicate multiple methods of prevention. Hear Res 2007;226:2243
7Ohinata, Y, Miller, JM, Altschuler, RA, Schacht, J. Intense noise induces formation of vasoactive lipid peroxidation products in the cochlea. Brain Res 2000;878:163–73
8Tuğtepe, H, Sener, G, Biyikli, NK, Yuksel, M, Cetinel, S, Gedik, N et al. The protective effect of oxytocin on renal ischemia/reperfusion injury in rats. Regul Pept 2007;140:101–8
9Dusunceli, F, Iseri, SO, Ercan, F, Gedik, N, Yegen, C, Yegen, BC. Oxytocin alleviates hepatic ischemia–reperfusion injury in rats. Peptides 2008;29:1216–22
10Elberry, AA, Refaie, SM, Kamel, M, Ali, T, Darwish, H, Ashourg, O. Oxytocin ameliorates cisplatin-induced nephrotoxicity in Wistar rats. Ann Saudi Med 2013;33:5762
11Ramesh, G, Reeves, WB. p38 MAP kinase inhibition ameliorates cisplatin nephrotoxicity in mice. Am J Physiol Renal Physiol 2005;289:F16674
12Tsuruya, K, Tokumoto, M, Ninomiya, T, Hirakawa, M, Masutani, K, Taniguchi, M et al. Antioxidant ameliorates cisplatin-induced renal tubular cell death through inhibition of death receptor-mediated pathways. Am J Physiol Renal Physiol 2003;285:F20818
13Bıyıklı, NK, Tuğtepe, H, Şener, G, Velioğlu-Öğünç, A, Çetinel, Ş, Gedik, N et al. Oxytocin alleviates oxidative renal injury in pyelonephritic rats via a neutrophil-dependent mechanism. Peptides 2006;27:2249–57
14Iseri, , Şener, G, Sağlam, B, Gedik, N, Ercan, F, Yeğen, BC. Oxytocin protects against sepsis-induced multiple organ damage: role of neutrophils. J Surg Res 2005;126:7381
15Rashed, LA, Hashem, RM, Soliman, HM. Oxytocin inhibits NADPH oxidase and P38 MAPK in cisplatin-induced nephrotoxicity. Biomed Pharmacother 2011;65:474–80
16Bekmez Bilmez, ZE, Aydin, S, Şanli, A, Altintoprak, N, Demir, MG, Atalay Erdoğan, B et al. Oxytocin as a protective agent in cisplatin-induced ototoxicity. Cancer Chemother Pharmacol 2016;77:875–9
17Hu, BH, Henderson, D, Nicotera, TM. Involvement of apoptosis in progression of cochlear lesion following exposure to intense noise. Hear Res 2002;166:6271
18Van Campen, LE, Murphy, WJ, Franks, JR, Mathias, PI, Toraason, MA. Oxidative DNA damage is associated with intense noise exposure in the rat. Hear Res 2002;164:2938
19Miller, JM, Brown, JN, Schacht, J. 8-iso-prostaglandin F(2alpha), a product of noise exposure, reduces inner ear blood flow. Audiol Neurootol 2003;8:207–21
20Kurioka, T, Matsunobu, T, Niwa, K, Tamura, A, Satoh, Y, Shiotani, A. Activated protein C rescues the cochlea from noise-induced hearing loss. Brain Res 2014;1583:201–10
21Aksoy, F, Dogan, R, Yenigun, A, Veyseller, B, Ozturan, O, Ozturk, B. Thymoquinone treatment for inner-ear acoustic trauma in rats. J Laryngol Otol 2015;129:3845
22Salt, AN, Plontke, SK. Local inner-ear drug delivery and pharmacokinetics. Drug Discov Today 2005;10:1299–306
23Wang, X, Dellamary, L, Fernandez, R, Harrop, A, Keithley, EM, Harris, JP et al. Dose-dependent sustained release of dexamethasone in inner ear cochlear fluids using a novel local delivery approach. Audiol Neurootol 2009;14:393401
24Yamane, H, Nakai, Y, Takayama, M, Iqucgi, H, Nakaqawa, T, Kojima, A. Appearance of free radicals in the guinea pig inner ear after noise-induced acoustic trauma. Eur Arch Otorhinolaryngol 1995;252:504–8
25Henderson, D, Bielefeld, EC, Harris, KC, Hu, BH. The role of oxidative stress in noise-induced hearing loss. Ear Hear 2006;27:119
26Davis, B, Qiu, W, Hamernik, R. Sensitivity of distortion product otoacoustic emissions in noise-exposed chinchillas. J Am Acad Audiol 2005;16:6978
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

The Journal of Laryngology & Otology
  • ISSN: 0022-2151
  • EISSN: 1748-5460
  • URL: /core/journals/journal-of-laryngology-and-otology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *



Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed