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Effect of local use of l-carnitine after myringotomy on myringosclerosis development in rats

Published online by Cambridge University Press:  03 April 2013

E Vuralkan ,
S Alicura Tokgöz ,
G Simsek ,
F Koybasioglu ,
U Han ,
M Caliskan ,
O Besalti  and
I Akin
E Vuralkan*
Affiliation:
Department of Otorhinolaryngology Clinic, Trabzon Kanuni Research and Training Hospital, Ankara, Turkey
S Alicura Tokgöz
Affiliation:
Department of Otorhinolaryngology (Clinic I), Diskapi Yildirim Beyazit Research and Training Hospital, Ankara, Turkey
G Simsek
Affiliation:
Department of Otorhinolaryngology (Clinic I), Diskapi Yildirim Beyazit Research and Training Hospital, Ankara, Turkey
F Koybasioglu
Affiliation:
Department of Pathology (Clinic I), Diskapi Yildirim Beyazit Research and Training Hospital, Ankara, Turkey
U Han
Affiliation:
Department of Pathology (Clinic I), Diskapi Yildirim Beyazit Research and Training Hospital, Ankara, Turkey
M Caliskan
Affiliation:
Faculty of Veterinary Medicine, Ankara University, Turkey
O Besalti
Affiliation:
Faculty of Veterinary Medicine, Ankara University, Turkey
I Akin
Affiliation:
Department of Otorhinolaryngology (Clinic I), Diskapi Yildirim Beyazit Research and Training Hospital, Ankara, Turkey
*
Address for correspondence: Dr Erkan Vuralkan, Department of Otorhinolaryngology Clinic, Trabzon Kanuni Research and Training Hospital, Kaşüstü Mevkii, Yomra, Trabzon, Turkey61290 Fax: +90 4622302307 E-mail: erkanvuralkan@hotmail.com
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Abstract

Objectives:

This study aimed to investigate the effect of local and intraperitoneal administration of l-carnitine on the prevention of experimentally induced myringosclerosis, and to compare treatment efficiency.

Methods:

Twenty-four Albino-Wistar rats (48 ears) were bilaterally myringotomised and divided randomly into four groups: group one received no treatment, group two received intraperitoneal l-carnitine, group three received local l-carnitine, and group four received both intraperitoneal and local l-carnitine. On the 15th day after treatment, tympanic membranes were harvested and evaluated histopathologically for myringosclerotic plaque formation, fibroblastic proliferation, tympanic membrane thickness and new vessel formation.

Results:

The group one tympanic membranes showed extensive thickness, and the incidence of myringosclerosis and fibroblast proliferation were greater than in groups two and four. There were statistically significant differences in tympanic membrane thickness between groups three and four, and in myringosclerosis incidence and fibroblast proliferation, comparing groups two, three and four.

Conclusion:

Myringosclerosis development was significantly reduced in rats receiving myringotomy plus intraperitoneal l-carnitine. Intraperitoneal l-carnitine administration prevented fibroblastic proliferation and tympanic membrane thickening (both of which cause further tympanic membrane destruction), thus reducing myringotomy-associated morbidity. Local l-carnitine administration had limited effectiveness in this experimental setting.

Keywords

l-carnitineMyringosclerosisTympanic MembraneRatsInflammation
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 127 , Issue 5 , May 2013 , pp. 468 - 472
Copyright
Copyright © JLO (1984) Limited 2013 

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