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Efficacy of autologous fat injection laryngoplasty with an adenoviral vector expressing hepatocyte growth factor in a canine model

Published online by Cambridge University Press:  22 May 2009

H Umeno ,
S Chitose ,
Y Murofushi ,
K Kosai ,
K Sato ,
A Kawahara  and
T Nakashima
H Umeno*
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, Kurume, Japan
S Chitose
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, Kurume, Japan
Y Murofushi
Affiliation:
Division of Gene Therapy and Regenerative Medicine, Department of Neuro-musculoskeletal Disorders, Kagoshima University Graduate School of Medical and Dental Sciences, Japan
K Kosai
Affiliation:
Division of Gene Therapy and Regenerative Medicine, Department of Neuro-musculoskeletal Disorders, Kagoshima University Graduate School of Medical and Dental Sciences, Japan
K Sato
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, Kurume, Japan
A Kawahara
Affiliation:
Department of Pathology, Kurume University School of Medicine, Kurume, Japan
T Nakashima
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, Kurume, Japan
*
Address for correspondence: Dr Hirohito Umeno, Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan. Fax: +81 942 37 1200 E-mail: umeno2@med.kurume-u.ac.jp
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Abstract

Objective:

The effectiveness of autologous fat injection laryngoplasty may be reduced by resorption of injected fat tissue. The aim of the present study was to clarify the efficacy of fat injection laryngoplasty using autologous fat plus a replication-defective adenoviral vector expressing hepatocyte growth factor, regarding reduction of injected fat tissue resorption.

Material and methods:

Four female beagle dogs were used in this study. After sedation, a direct laryngoscope was introduced to enable visualisation of the larynx. In each dog, harvested autologous fat plus an adenoviral vector expressing hepatocyte growth factor was injected into the right true vocal fold, and harvested fat plus an adenoviral vector expressing no gene was injected into the left true vocal fold. A total laryngectomy was performed one year after the intracordal fat injection. Coronal sections of the resected whole larynges were made and the following parameters assessed using light and electron microscopy: size of fat area; number of vasculoendothelial cells surrounding adipocytes; and shape of injected adipocytes in the vocal fold.

Results:

The fat area was significantly larger and the number of vasculoendothelial cells surrounding adipocytes significantly greater in the intracordal fat injection containing adenoviral vector expressing hepatocyte growth factor, compared with the control intracordal fat injection containing adenoviral vector expressing no gene. When viewed under electron microscopy, the injected adipocytes were observed to have grafted better in the intracordal fat injection with hepatocyte growth factor adenoviral vector, compared with the control intracordal fat injection with adenoviral vector expressing no gene.

Conclusions:

Injection into the vocal fold of autologous fat containing an adenoviral vector expressing hepatocyte growth factor can reduce subsequent resorption of injected fat.

Keywords

Autologous Fat Injection LaryngoplastyAdenoviral VectorHepatocyte Growth FactorVasculoendothelial CellAdipocytes
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 123 , Supplement S31 , May 2009 , pp. 24 - 29
Copyright
Copyright © JLO (1984) Limited 2009

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References

1Umeno, H, Shirouzu, H, Chitose, S, Nakashima, T. Analysis of voice function following autologous fat injection for vocal fold paralysis. Otolaryngol Head Neck Surg 2005;132:103–7CrossRefGoogle ScholarPubMed
2Umeno, H, Sato, K, Shirouzu, H, Chitose, S, Nakashima, T. Current state and future of autologous fat injection laryngoplasty – based on voice comparison between autologous fat injection laryngoplasty and framework surgery [in Japanese]. Jpn J Logop Phoniatr 2007;48:163–70CrossRefGoogle Scholar
3Umeno, H, Sato, K, Shirouzu, H, Chitose, S, Nakashima, T. Fundamental and clinical medicine for fat injection laryngoplasty [in Japanese]. Larynx Jpn 2006;18:89–95CrossRefGoogle Scholar
4Sato, K, Umeno, H, Nakashima, T. Histological investigation of liposuctioned fat for injection laryngoplasty. Am J Otolaryngol 2005;26:219–25CrossRefGoogle ScholarPubMed
5Matsumoto, K, Nakamura, T. Hepatocyte growth factor (HGF) as a tissue organizer for organogenesis and regeneration. Biochem Biophys Res Commun 1997;239:639–44Google ScholarPubMed
6Birchmeier, C, Gherardi, E. Developmental roles of HGF/SF and its receptor, the c-Met tyrosine kinase. Trends Cell Biol 1998;8:404–10CrossRefGoogle ScholarPubMed
7Li, Y, Takemura, G, Kosai, K, Yuge, K, Nagano, S, Esaki, M et al. Postinfarction treatment with an adenoviral vector expressing hepatocyte growth factor relieves chronic left ventricular remodeling and dysfunction in mice. Circulation 2003;107:2499–506CrossRefGoogle ScholarPubMed
8Yuge, K, Takahashi, T, Nagano, S, Terazaki, Y, Murofushi, Y, Ushikoshi, H, Kawai, T, Khai, NC, Nakamura, T, Fujiwara, H, Kosai, K. Adenoviral gene transduction of hepatocyte growth factor elicits inhibitory effects for hepatoma. Int J Oncol 2005;27:77–85Google ScholarPubMed
9Murofushi, Y, Nagano, S, Kamizono, J, Takahashi, T, Fujiwara, H, Komiya, S, Matsuishi, T, Kosai, K. Cell cycle-specific changes in hTERT promoter activity in normal and cancerous cells in adenoviral gene therapy: A promising implication of telomerase-dependent targeted cancer gene therapy. Int J Oncol 2006;29:681–8Google ScholarPubMed
10Mikaelian, DO, Lowry, LD, Sataloff, RT. Lipoinjection for unilateral vocal cord paralysis. Laryngoscope 1991;101:465–8CrossRefGoogle ScholarPubMed
11Hsiung, MW, Pai, LU. Autologous fat injection for glottic insufficiency: analysis of 101 cases and correlation with patient's self-assessment. Acta Otolaryngol 2006;126:191–6CrossRefGoogle Scholar
12Tamura, E, Fukuda, H, Tabata, Y. Adipose tissue formation in response to basic fibroblast growth factor. Acta Otolaryngol 2007;127:1327–31CrossRefGoogle ScholarPubMed
13Nakamura, T, Nawa, K, Ichihara, A. Partial purification and characterization of hepatocyte growth factor from serum of hepatectomized rats. Biochem Biophys Res Commun 1984;122:1450–9CrossRefGoogle ScholarPubMed
14Nakamura, T, Nishizawa, T, Hagiya, M, Seki, T, Shimonishi, M, Sugimura, A et al. Molecular cloning and expression of human hepatocyte growth factor. Nature 1989;342:440–3CrossRefGoogle ScholarPubMed