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Improving cellular migration in tissue-engineered laryngeal scaffolds

Published online by Cambridge University Press:  21 March 2019

K Wismayer ,
N Mehrban ,
J Bowen  and
M Birchall
K Wismayer*
Affiliation:
Division of Surgery, Ear Institute, University College London, UK
N Mehrban
Affiliation:
Division of Surgery, Ear Institute, University College London, UK
J Bowen
Affiliation:
School of Engineering and Innovation, Open University, Milton Keynes, UK
M Birchall
Affiliation:
Ear Institute, University College London, UK
*
Author for correspondence: Dr Kurt Wismayer, Doctor's Office, 10 South Ward, Charing Cross Hospital, Fulham Palace Road, London W6 8RF, UK E-mail: kurtwism1989@gmail.com
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Abstract

Objective

To modify the non-porous surface membrane of a tissue-engineered laryngeal scaffold to allow effective cell entry.

Methods

The mechanical properties, surface topography and chemistry of polyhedral oligomeric silsesquioxane poly(carbonate-urea) urethane were characterised. A laser technique introduced surface perforations. Micro computed tomography generated porosity data. Scaffolds were seeded with cells, investigated histologically and proliferation studied. Incubation and time effects were assessed.

Results

Laser cutting perforated the polymer, connecting the substructure with the ex-scaffold environment and increasing porosity (porous, non-perforated = 87.9 per cent; porous, laser-perforated at intensities 3 = 96.4 per cent and 6 = 89.5 per cent). Cellular studies confirmed improved cell viability. Histology showed cells adherent to the scaffold surface and cells within perforations, and indicated that cells migrated into the scaffolds. After 15 days of incubation, scanning electron microscopy revealed an 11 per cent reduction in pore diameter, correlating with a decrease in Young's modulus.

Conclusion

Introducing surface perforations presents a viable method of improving polyhedral oligomeric silsesquioxane poly(carbonate-urea) urethane as a tissue-engineered scaffold.

Keywords

Tissue EngineeringLaryngeal NeoplasmsPorosityCell ProliferationNanocompositesTissue Scaffolds
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 133 , Issue 2 , February 2019 , pp. 135 - 148
Copyright
Copyright © JLO (1984) Limited, 2019 

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Footnotes

Dr K Wismayer takes responsibility for the integrity of the content of the paper

Presented at the Association of Surgeons in Training conference, 31 March – 2 April 2017, Bournemouth, UK.

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