Skip to main content Accessibility help

MicroRNA let-7a up-regulates OPN expression in a mouse model of allergic rhinitis

  • H Yu (a1), H Sun (a2), Z Wang (a3) and Y Liu (a3)



To investigate the effect of microRNA let-7a on OPN expression in a mouse model of allergic rhinitis.


Thirty-two mature female C57BL/6 mice were randomly divided into four groups, with eight mice in each group: microRNA let-7a, microRNA control, OVA and phosphate-buffered saline groups. The microRNA let-7a, microRNA control and OVA groups were sensitised with OVA to establish a mouse model of allergic rhinitis. Nose-scratching events were counted. Nasal mucosa was used to evaluate histological changes of goblet cell hyperplasia. Interleukins 5 and 13 were detected. Interferon-γ levels in the nasal lavage fluid were assayed with enzyme-linked immunosorbent assay. OPN expression was estimated with polymerase chain reaction.


Compared with microRNA control mice, microRNA let-7a treated mice had a significantly increased number of nose-scratching events, nasal mucosal eosinophilia and goblet cell hyperplasia (p < 0.05), and significantly higher interleukins 5 and 13 in nasal mucosa (p < 0.05), but there was no significant difference in interferon-γ (p > 0.05). In addition, microRNA let-7a treated mice had significantly enhanced OPN expression in nasal mucosa (p < 0.05).


MicroRNA let-7a can promote allergic rhinitis development partly by regulating OPN expression.


Corresponding author

Address for correspondence: Dr Yang Liu, Department of Otolaryngology – Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430030, P R China E-mail:


Hide All
1 Greiner, AN, Hellings, PW, Rotiroti, G, Scadding, GK. Allergic rhinitis. Lancet 2011;378:2112–22
2 Bousquet, J, Khaltaev, N, Cruz, AA, Denburg, J, Fokkens, WJ, Togias, A et al. Allergic Rhinitis and its Impact on Asthma (ARIA) 2008 update (in collaboration with the World Health Organization, GA2LEN and AllerGen). Allergy 2008;63:8160
3 Zhang, YM, Zhang, J, Liu, SL, Zhang, X, Yang, SN, Gao, J et al. Prevalence and associated risk factors of allergic rhinitis in preschool children in Beijing. Laryngoscope 2013;123:2835
4 Rosenwasser, LJ. Current understanding of the pathophysiology of allergic rhinitis. Immunol Allergy Clin North Am 2011;31:433–9
5 Ambros, V. MicroRNAs: tiny regulators with great potential. Cell 2001;107:823–6
6 Ambros, V. The functions of animal microRNAs. Nature 2004;431:350–5
7 Bartel, DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 2004;116:281–97
8 Calin, GA, Dumitru, CD, Shimizu, M, Bichi, R, Zupo, S, Noch, E et al. Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia. Proc Natl Acad Sci U S A 2002;99:15524–9
9 Yang, M, Li, Y, Padgett, RW. MicroRNAs: small regulators with a big impact. Cytokine Growth Factor Rev 2005;16:387–93
10 Liu, Y, Lu, X, Yu, HJ, Hua, XY, Cui, YH, Huang, SK et al. The expression of osteopontin and its association with Clara cell 10 kDa protein in allergic rhinitis. Clin Exp Allergy 2010;40:1632–41
11 Makino, K, Jinnin, M, Hirano, A, Yamane, K, Eto, M, Kusano, T et al. The downregulation of microRNA let-7a contributes to the excessive expression of type I collagen in systemic and localized scleroderma. J Immunol 2013;190:3905–15
12 Liu, Y, Yu, HJ, Wang, N, Zhang, YN, Huang, SK, Cui, YH et al. Clara cell 10-kDa protein inhibits T(H)17 responses through modulating dendritic cells in the setting of allergic rhinitis. J Allergy Clin Immunol 2013;131:387–94
13 Kim, YH, Yang, TY, Park, CS, Ahn, SH, Son, BK, Kim, JH et al. Anti-IL-33 antibody has a therapeutic effect in a murine model of allergic rhinitis. Allergy 2012;67:183–90
14 Roggli, E, Britan, A, Gattesco, S, Lin-Marq, N, Abderrahmani, A, Meda, P et al. Involvement of microRNAs in the cytotoxic effects exerted by proinflammatory cytokines on pancreatic beta-cells. Diabetes 2010;59:978–86
15 Lu, TX, Munitz, A, Rothenberg, ME. MicroRNA-21 is up-regulated in allergic airway inflammation and regulates IL-12p35 expression. J Immunol 2009;182:49945002
16 Lu, TX, Hartner, J, Lim, EJ, Fabry, V, Mingler, MK, Cole, ET et al. MicroRNA-21 limits in vivo immune response-mediated activation of the IL-12/IFN-gamma pathway, Th1 polarization, and the severity of delayed-type hypersensitivity. J Immunol 2011;187:3362–73
17 Shell, S, Park, SM, Radjdai, AR, Schickel, R, Kistner, EO, Jewell, DA et al. Let-7 expression defines two differentiation stages of cancer. Proc Natl Acad Sci USA 2007;104:11400–5
18 Polikepahad, S, Knight, JM, Naghavi, AO, Oplt, T, Creighton, CJ, Shaw, C et al. Proinflammatory role for let-7 microRNAS in experimental asthma. J Biol Chem 2010;285:30139–49
19 Cantor, H, Shinohara, ML. Regulation of T-helper-cell lineage development by osteopontin: the inside story. Nat Rev Immunol 2009;9:137–41
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

The Journal of Laryngology & Otology
  • ISSN: 0022-2151
  • EISSN: 1748-5460
  • URL: /core/journals/journal-of-laryngology-and-otology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *



Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed