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Therapeutic and protective effects of autologous serum in amikacin-induced ototoxicity

  • I B Arslan (a1), G G Aslan (a1), G C Mercan (a1), S Vatansever (a2), I Cukurova (a1), S Gokalp (a2) and A Aslan (a3)...

Abstract

Objective:

Possible therapeutic and protective benefits of intratympanic autologous serum application in amikacin-induced ototoxicity were investigated.

Methods:

Twenty-four guinea pigs were separated equally into two groups: therapeutic (group A) and protective (group B). Transient evoked otoacoustic emissions were recorded before and after autologous serum application. Apoptotic cells were identified in the organ of Corti, spiral limbus and spiral ganglion by the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (‘TUNEL’) method.

Results:

Transient evoked otoacoustic emission responses at 1, 1.4 and 2.8 kHz improved without significance after autologous serum application in group A (p > 0.05). A significantly protective effect of autologous serum was determined at 4 kHz in group B (p < 0.05). There were significantly fewer apoptotic cells at the spiral limbus in the therapeutic and protective groups compared to the control group (p < 0.05).

Conclusion:

Autologous serum may offer protection against ototoxicity-induced hearing loss, but it cannot restore hearing. Immunohistochemically, autologous serum significantly decreases activation of the intrinsic pathway of pro-apoptotic signalling in mesenchymal cells compared to neurons and neurosensory cells.

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Copyright

Corresponding author

Address for correspondence: Dr Ilker Burak Arslan, Izmir Tepecik Egitim ve Arastirma Hastanesi KBB Klinigi, Gaziler cad. No: 468, 35170 Yenisehir, Izmir, Turkey Fax: +90 232 4330756 E-mail: ilkerburakarslan@hotmail.com

Footnotes

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Presented at the 30th Politzer Society Meeting, 30 June – 4 July 2015, Niigata, Japan.

Footnotes

References

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1 Shi, L, An, Y, Wang, A, Gao, Q, Yang, Y. The protective effect of Salvia miltiorrhiza on gentamicin-induced ototoxicity. Am J Otolaryngol 2014;35:171–9
2 Polony, G, Humli, V, Ando, R, Aller, M, Horvalth, T, Harnos, A et al. Protective effect of rasagiline in aminoglycoside ototoxicity. Neuroscience 2014;265:263–73
3 Berkiten, G, Slturk, Z, Topaloglu, I, Ugras, H. Protective effect of pentoxifylline on amikacin-induced ototoxicity in rats. Am J Otolaryngol 2012;33:689–92
4 Guthrie, OW. Aminoglycoside induced ototoxicity. Toxicology 2008;249:91–6
5 Blakley, BW, Alsaleh, S, Dewji, Z, Qureshy, K, Berard, S, Xie, L. Steroids in aminoglycoside-containing ear drops: do they reduce cochlear toxicity? Laryngoscope 2014;124:1209–13
6 Kocyigit, I, Vural, A, Unal, A, Sipahioglu, MH, Yucel, HE, Aydemir, S et al. Preventing amikacin related ototoxicity with N-acetylcysteine in patients undergoing peritoneal dialysis. Eur Arch Otorhinolaryngol 2015;272:2611–20
7 Kelly, KM, Lalwani, AK. On the distant horizon – medical therapy for sensorineural hearing loss. Otolaryngol Clin North Am 2015;48:1149–65
8 Fortier, LA, Barker, JU, Strauss, EJ, McCarrel, TM, Cole, BJ. The role of growth factors in cartilage repair. Clin Orthop Relat Res 2011;469:2706–15
9 Wehling, P, Moser, C, Frisbie, D, McIlwraith, W, Kawcak, CE, Krauspe, R et al. Autologous conditioned serum in the treatment of orthopedic diseases: the orthokine therapy. BioDrugs 2007;21:323–32
10 Lang, UE, Gallinat, J, Danker-Hopfe, H, Bajbouj, M, Hellweg, R. Nerve growth factor serum concentrations in healthy human volunteers: physiological variance and stability. Neurosci Lett 2003;344:1316
11 Matsumoto, Y, Dogru, M, Goto, E, Ohashi, Y, Kojima, T, Ishida, R et al. Autologous serum application in the treatment of neurotrophic keratopathy. Ophthalmology 2004;11:1115–20
12 Baltzer, AW, Ostapczuk, MS, Stosch, D, Seidel, F, Granrath, M. A new treatment for hip osteoarthritis: clinical evidence for the efficacy of autologous conditioned serum. Orthop Rev (Pavia) 2013;5:5964
13 Kakehata, S, Hirose, Y, Kitani, R, Futai, K, Maruya, S, Ishii, K et al. Autologous serum eardrops therapy with a chitin membrane for closing tympanic membrane perforations. Otol Neurotol 2008;29:791–5
14 Bomstein, Y, Yuklea, M, Radnay, J, Shapiro, H, Afanasyev, F, Yarkoni, S et al. The antiapoptotic effects of blood constituents in patients with chronic lymphocytic leukemia. Eur J Haematol 2003;70:290–5
15 Kaevalin, LK, Jongkhajornpong, P, Choubtum, L, Chuckpaiwong, V. Topical 100% serum eye drops for treating corneal epithelial defect after ocular surgery. BioMed Res Int 2013;2013:521315
16 Duan, WR, Garner, DS, Williams, SD, Funckes-Shippy, CL, Spath, IS, Blomme, EA. Comparison of immunohistochemistry for activated caspase-3 and cleaved cytokeratin 18 with the TUNEL method for quantification of apoptosis in histological sections of PC-3 subcutaneous xenografts. J Pathol 2003;199:221–8
17 Derinsu, U, Akdas, F. TEOAE findings of guinea pigs [in Turkish]. Otoskop 2003;3:127–31
18 Vatansever, HS, Sorkun, K, Guran, SI, Ozdal-Kurt, F, Turkoz, E, Gencay, O et al. Propolis from Turkey induces apoptosis through activating caspases in human breast carcinoma cell lines. Acta Histochem 2010;112:546–56
19 Forge, A, Li, L. Apoptotic death of hair cells in mammalian vestibular sensory epithelia. Hear Res 2000;139:97115
20 Williams, JA, Holder, N. Cell turnover in neuromasts of zebrafish larvae. Hear Res 2000;143:171–81
21 Nakamagoe, M, Tabuchi, K, Uemaetomari, I, Nishimura, B, Hara, A. Estradiol protects the cochlea against gentamicin ototoxicity through inhibition of the JNK pathway. Hear Res 2010;261:6774
22 Olsson, M, Zhivotovsky, B. Caspases and cancer. Cell Death Differ 2011;18:1441–9
23 Campbell, KC, Meech, RP, Klemens, JJ, Gerberi, MT, Dyrstad, SS, Larsen, DL et al. Prevention of noise- and drug-induced hearing loss with D-methionine. Hear Res 2007;226:92103
24 Erdem, T, Ozturan, O, Iraz, M, Miman, MC, Olmez, E. Dose-dependent dual effect of melatonin on ototoxicity induced by amikacin in adult rats. Eur Arch Otorhinolaryngol 2005;262:314–21
25 Gillespie, LN, Clark, GM, Bartlett, PF, Marzella, PL. BDNF-induced survival of auditory neurons in vivo: cessation of treatment leads to accelerated loss of survival effects. J Neurosci Res 2003;71:785–90
26 Quinto, GG, Campos, M, Behrens, A. Autologous serum for ocular surface diseases. Arq Bras Oftalmol 2008;71:4754
27 Gunay, C, Sagliyan, A, Yilmaz, S, Kandemir, FM, Han, MC, Ozkaraca, M et al. Evaluation of autologous serum eyedrops for the treatment of experimentally induced corneal alcali burns. Revue Méd Vét 2015;166:6371
28 Poon, AC, Geerling, G, Dart, JK, Fraenkel, GE, Daniels, JT. Autologous serum eyedrops for dry eyes and epithelial defects: clinical and in vitro toxicity studies. Br J Ophthalmol 2001;85:1188–97
29 Kamal, S, Kumar, S, Goel, R. Autologous serum for anterior tissue necrosis after porous orbital implant. Middle East Afr J Ophthalmol 2014;21:193–5
30 Wehling, P, Moser, C, Frisbie, D, McIlwraith, CW, Kawcak, CE, Krauspe, R et al. Autologous conditioned serum in the treatment of orthopedic diseases: the orthokine therapy. BioDrugs 2007;21:323–32
31 Nimura, A, Muneta, T, Koga, H, Mochizuki, T, Suzuki, K, Makino, H et al. Increased proliferation of human synovial mesenchymal stem cells with autologous human serum: comparisons with bone marrow mesenchymal stem cells and with fetal bovine serum. Arthritis Rheum 2008;58:501–10
32 Geerling, G, Maclennan, S, Hartwig, D. Autologous serum eye drops for ocular surface disorders. Br J Ophthalmol 2004;88:1467–74
33 Huth, ME, Ricci, J, Cheng, AG. Mechanisms of aminoglycoside ototoxicity and targets of hair cell protection. Int J Otolaryngol 2011;2011:937861
34 Alharazneh, A, Luk, L, Huth, M, Monfared, A, Steyger, PS, Cheng, AG et al. Functional hair cell mechanotransducer channels are required for aminoglycoside ototoxicity. PLoS ONE 2011;6:e22347
35 Kikkawa, YS, Nakagawa, T, Ying, L, Tabata, Y, Tsubouchi, H, Ido, A et al. Growth factor-eluting cochlear implant electrode: impact on residual auditory function, insertional trauma, and fibrosis. J Transl Med 2014;12:280
36 Kimura, RS, Nye, CL, Southard, RE. Normal and pathologic features of the limbus spiralis and its functional significance. Am J Otolaryngol 1990;11:99111
37 Cho, HH, Jang, S, Lee, SC, Jeong, HS, Park, JS, Han, JY et al. Effect of neural-induced mesenchymal stem cells and platelet-rich plasma on facial nerve regeneration in an acute nerve injury model. Laryngoscope 2010;120:907–13
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