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The use of fluorescence lifetime technology in benign and malignant thyroid tissues

  • G Nakache (a1), G Yahav (a2), G H Siloni (a3), I Barshack (a4), E Alon (a5), M Wolf (a5) and D Fixler (a2)...



To explore the use of fluorescence lifetime imaging microscopy in thyroid tissues, and to investigate how different thyroid lesions affect fluorescence lifetime.


Fluorescence lifetime measurements were taken of fresh frozen thyroid surgical specimens stained with fluorescein isothiocyanate tagged anti-thyroglobulin monoclonal antibodies.


The mean fluorescence lifetime measurements in 12 patients – 3 with multinodular goitre, 4 with follicular adenoma, 4 with papillary thyroid carcinoma and 1 with follicular carcinoma – were 3.16 ns (range, 2.66–3.52 ns), 3.75 ns (range, 2.99–4.57 ns), 2.97 ns (range, 2.57–3.21 ns) and 3.61 ns, respectively. The fluorescence lifetime of follicular adenoma patients was higher than that of papillary thyroid carcinoma patients by 26 per cent (p = 0.058). The fluorescence lifetime in the follicular carcinoma patient was similar to the follicular adenoma group, but higher than in the papillary thyroid carcinoma group by 22 per cent (p = 0.01).


Fluorescence lifetime measurements varied in different thyroid pathologies, possibly because of tissue-scale structural influences.


Corresponding author

Author for correspondence: Dr Gabriel Nakache, Department of Otolaryngology Head and Neck Surgery, Rabin Medical Center, Petach Tikva, Israel E-mail:


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Dr G Nakache takes responsibility for the integrity of the content of the paper



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