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The use of fluorescence lifetime technology in benign and malignant thyroid tissues

  • G Nakache (a1), G Yahav (a2), G H Siloni (a3), I Barshack (a4), E Alon (a5), M Wolf (a5) and D Fixler (a2)...

Abstract

Objective

To explore the use of fluorescence lifetime imaging microscopy in thyroid tissues, and to investigate how different thyroid lesions affect fluorescence lifetime.

Method

Fluorescence lifetime measurements were taken of fresh frozen thyroid surgical specimens stained with fluorescein isothiocyanate tagged anti-thyroglobulin monoclonal antibodies.

Results

The mean fluorescence lifetime measurements in 12 patients – 3 with multinodular goitre, 4 with follicular adenoma, 4 with papillary thyroid carcinoma and 1 with follicular carcinoma – were 3.16 ns (range, 2.66–3.52 ns), 3.75 ns (range, 2.99–4.57 ns), 2.97 ns (range, 2.57–3.21 ns) and 3.61 ns, respectively. The fluorescence lifetime of follicular adenoma patients was higher than that of papillary thyroid carcinoma patients by 26 per cent (p = 0.058). The fluorescence lifetime in the follicular carcinoma patient was similar to the follicular adenoma group, but higher than in the papillary thyroid carcinoma group by 22 per cent (p = 0.01).

Conclusion

Fluorescence lifetime measurements varied in different thyroid pathologies, possibly because of tissue-scale structural influences.

Copyright

Corresponding author

Author for correspondence: Dr Gabriel Nakache, Department of Otolaryngology Head and Neck Surgery, Rabin Medical Center, Petach Tikva, Israel E-mail: nakachega@gmail.com

Footnotes

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Dr G Nakache takes responsibility for the integrity of the content of the paper

Footnotes

References

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1Haugen, BR, Alexander, EK, Bible, KC, Doherty, GM, Mandel, SJ, Nikiforov, YE et al. 2015 American Thyroid Association Management Guidelines for Adult Patients with Thyroid Nodules and Differentiated Thyroid Cancer: The American Thyroid Association Guidelines Task Force on Thyroid Nodules and Differentiated Thyroid Cancer. Thyroid 2016;26:1133
2Marqusee, E, Benson, CB, Frates, MC, Doubilet, PM, Larsen, PR, Cibas, ES et al. Usefulness of ultrasonography in the management of nodular thyroid disease. Ann Intern Med 2000;133:696700
3Brito, JP, Gionfriddo, MR, Al, NA, Boehmer, KR, Leppin, AL, Reading, C et al. The accuracy of thyroid nodule ultrasound to predict thyroid cancer: systematic review and meta-analysis. J Clin Endocrinol Metab 2014;99:1253–63
4Danese, D, Sciacchitano, S, Farsetti, A, Andreoli, M, Pontecorvi, A. Diagnostic accuracy of conventional versus sonography-guided fine-needle aspiration biopsy of thyroid nodules. Thyroid 1998;8:1521
5Cibas, ES, Ali, SZ. The Bethesda System for Reporting Thyroid Cytopathology. Am J Clin Pathol 2009;132:658–65
6Bongiovanni, M, Spitale, A, Faquin, WC, Mazzucchelli, L, Baloch, ZW. The Bethesda System for Reporting Thyroid Cytopathology: a meta-analysis. Acta Cytol 2012;56:333–9
7Ohori, NP, Schoedel, KE. Variability in the atypia of undetermined significance/follicular lesion of undetermined significance diagnosis in the Bethesda System for Reporting Thyroid Cytopathology: sources and recommendations. Acta Cytol 2011;55:492–8
8Braga, M, Cavalcanti, TC, Collaco, LM, Graf, H. Efficacy of ultrasound-guided fine-needle aspiration biopsy in the diagnosis of complex thyroid nodules. J Clin Endocrinol Metab 2001;86:4089–91
9Alexander, EK, Heering, JP, Benson, CB, Frates, MC, Doubilet, PM, Cibas, ES et al. Assessment of nondiagnostic ultrasound-guided fine needle aspirations of thyroid nodules. J Clin Endocrinol Metab 2002;87:4924–7
10Kroll, TG, Sarraf, P, Pecciarini, L, Chen, CJ, Mueller, E, Spiegelman, BM et al. PAX8-PPAR gamma1 fusion oncogene in human thyroid carcinoma. Science 2000;289:1357–60
11Umbricht, CB, Saji, M, Westra, WH, Udelsman, R, Zeiger, MA, Sukumar, S. Telomerase activity: a marker to distinguish follicular thyroid adenoma from carcinoma. Cancer Res 1997;57:2144–7
12Nikiforov, YE. Role of molecular markers in thyroid nodule management: then and now. Endocr Pract 2017;23:979–88
13Febbo, PG, Ladanyi, M, Aldape, KD, De Marzo, AM, Hammond, ME, Hayes, DF et al. NCCN Task Force report: evaluating the clinical utility of tumor markers in oncology. J Natl Compr Canc Netw 2011;9(suppl 5):S132
14Xing, M, Haugen, BR, Schlumberger, M. Progress in molecular-based management of differentiated thyroid cancer. Lancet 2013;381:1058–69
15Ferris, RL, Baloch, Z, Bernet, V, Chen, A, Fahey, TJ 3rd, Ganly, I et al. ; American Thyroid Association Surgical Affairs Committee. American Thyroid Association Statement on Surgical Application of Molecular Profiling for Thyroid Nodules: current impact on perioperative decision making. Thyroid 2015;25:760–8
16Chang, C, Sud, D, Mycek, MA. Fluorescence lifetime imaging microscopy. Methods Cell Biol 2007;81:495524
17Bastiaens, PI, Squire, A. Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell. Trends Cell Biol 1999;9:4852
18Fixler, D, Tirosh, R, Shainberg, A, Deutsch, M. Cytoplasmic changes in cardiac cells during a contraction cycle detected by fluorescence polarization. J Fluoresc 2001;11:89100
19Berezin, MY, Achilefu, S. Fluorescence lifetime measurements and biological imaging. Chem Rev 2010;110:2641–84
20Colasanti, A, Kisslinger, A, Fabbrocini, G, Liuzzi, R, Quarto, M, Riccio, P et al. MS-2 fibrosarcoma characterization by laser induced autofluorescence. Lasers Surg Med 2000;26:441–8
21Yahav, G, Hirshberg, A, Salomon, O, Amariglio, N, Trakhtenbrot, L, Fixler, D. Fluorescence lifetime imaging of DAPI-stained nuclei as a novel diagnostic tool for the detection and classification of B-cell chronic lymphocytic leukemia. Cytometry A 2016;89:644–52
22Pradhan, A, Pal, P, Durocher, G, Villeneuve, L, Balassy, A, Babai, F et al. Steady state and time-resolved fluorescence properties of metastatic and non-metastatic malignant cells from different species. J Photochem Photobiol B 1995;31:101–12
23Zahavi, T, Yahav, G, Shimshon, Y, Gershanov, S, Kaduri, L, Sonnenblick, A et al. Utilizing fluorescent life time imaging microscopy technology for identify carriers of BRCA2 mutation. Biochem Biophys Res Commun 2016;480:3641
24Gershanov, S, Michowiz, S, Toledano, H, Yahav, G, Barinfeld, O, Hirshberg, A et al. Fluorescence lifetime imaging microscopy, a novel diagnostic tool for metastatic cell detection in the cerebrospinal fluid of children with medulloblastoma. Sci Rep 2017;7:3648
25Giubileo, F, Colao, A, Puiu, G, Panzironi, G, Brizzi, F, Rocchini, P. Fluorescence spectroscopy of normal and follicular cancer samples from human thyroid. Spectroscopy 2005;19:7987
26Brandao, M, Iwakura, R, Basilio, F, Haleplian, K, Ito, A, de Freitas, LC et al. Fluorescence lifetime of normal, benign, and malignant thyroid tissues. J Biomed Opt 2015;20:16
27Magde, D, Rojas, GE, Seybold, PG. Solvent dependence of the fluorescence lifetimes of xanthene dyes. Photochem Photobiol 1999;70:737–44

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