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Optical Waveguide Biosensors for Highly Sensitive and High-ThroughputApplications

Published online by Cambridge University Press:  28 March 2016

Ikuo Uematsu ,
Ichiro Tohno ,
Shingo Kasai ,
Masaaki Hirakawa ,
Kayoko Omiya  and
Hidetoshi Matsumoto
Ikuo Uematsu*
Affiliation:
Department of Organic and Polymeric Materials, Tokyo Institute of Technology, Mail Box S8-27, 2-12-1 Ookayama, Meguro-ku, Tokyo 152-8552, Japan Corporate Manufacturing Engineering Center, Toshiba Corporation, 33 Shin-Isogo-Cho, Isogo-ku, Yokohama 235-0017, Japan
Ichiro Tohno
Affiliation:
Corporate Manufacturing Engineering Center, Toshiba Corporation, 33 Shin-Isogo-Cho, Isogo-ku, Yokohama 235-0017, Japan
Shingo Kasai
Affiliation:
Corporate Manufacturing Engineering Center, Toshiba Corporation, 33 Shin-Isogo-Cho, Isogo-ku, Yokohama 235-0017, Japan
Masaaki Hirakawa
Affiliation:
Corporate Manufacturing Engineering Center, Toshiba Corporation, 33 Shin-Isogo-Cho, Isogo-ku, Yokohama 235-0017, Japan
Kayoko Omiya
Affiliation:
Corporate Manufacturing Engineering Center, Toshiba Corporation, 33 Shin-Isogo-Cho, Isogo-ku, Yokohama 235-0017, Japan
Hidetoshi Matsumoto
Affiliation:
Department of Organic and Polymeric Materials, Tokyo Institute of Technology, Mail Box S8-27, 2-12-1 Ookayama, Meguro-ku, Tokyo 152-8552, Japan
*
*(Email: uematsu.i.aa@m.titech.ac.jp)
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Abstract

In the present study, highly sensitive and high-throughput optical waveguidebiosensors were fabricated by using the sensing membranes containing dye andpolymer-enzyme complex. Optical light waveguide can detect the optical change inthe vicinity of the guide surface with high sensitivity due to the evanescentwave scattering. The glucose sensing membranes, composed of dye, enzymes, andbiocompatible polymers were prepared by solution processing on the opticalwaveguide. Herein, we used 3, 3’, 5,5’-tetramethylbenzidine (TMBZ) as a dye, glucose oxidase (GOD) andperoxidase (POD) as enzymes, phosphatide polymer for protection of biologicalactivity of enzyme, and carboxymethyl cellulose (CMC) as a binder. Then wefocused on the optimal composition and structure of sensing membranes for theenhancement in the sensitivity and response speed. The developed glucose sensorsdemonstrated 20 times higher sensitivity than the conventional light waveguideglucose sensors and the low-detection limit of 0.1g/L glucose within thedetection time of 60 sec. For further improvement in the sensitivity,microporous sensing membranes were fabricated by using electrosprayingtechnique. The electroprayed sensing membranes gave 40 % higher sensitivity thannonporous sensing membranes. These results show that both the composition andstructure of sensing membrane are crucial factors for highly sensitive andhigh-throughput optical waveguide biosensors.

Keywords

sensorbiomaterialspray deposition

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Type
Articles
Information
MRS Advances , Volume 1 , Issue 11: Nanomaterials and Synthesis , 2016 , pp. 755 - 760
Copyright
Copyright © Materials Research Society 2016 

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References

REFERENCES

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