Hostname: page-component-77c78cf97d-4gwwn Total loading time: 0 Render date: 2026-04-24T23:55:28.110Z Has data issue: false hasContentIssue false
  • English
  • Français

Super-Critical-CO2 De-ECM Process

Published online by Cambridge University Press:  26 June 2018

Diana Cho ,
Seungwon Chung ,
Jaeseok Eo  and
Namsoo P. Kim
Diana Cho
Affiliation:
Center for Printable Materials Certification, The University of Texas at El Paso, El Paso, TX, USA
Seungwon Chung
Affiliation:
Materials and Biomedical Engineering Department, The University of Texas at El Paso, El Paso, TX, USA
Jaeseok Eo
Affiliation:
Materials and Biomedical Engineering Department, The University of Texas at El Paso, El Paso, TX, USA
Namsoo P. Kim*
Affiliation:
Center for Printable Materials Certification, The University of Texas at El Paso, El Paso, TX, USA Materials and Biomedical Engineering Department, The University of Texas at El Paso, El Paso, TX, USA
*
*(Email: nkim@utep.edu)
Get access

Abstract

Extracellular Matrix (ECM), a natural biomaterials, have recently garnered attention in tissue engineering for their high degree of cell proliferative capacity, biocompatibility, biodegradability, and tenability in the body. Decellularization process offers a unique approach for fabricating ECM-based natural scaffold for tissue engineering application by removing intracellular contents in a tissue that could cause any adverse host responses. The effects of Supercritical carbon dioxide (Sc-CO2) treatment on the histological and biochemical properties of the decellularized extracellular matrix (de-ECM) were evaluated and compared with de-ECM from conventional decellularization process to see if it offers significantly reduced treatment times, complete decellularization, and well preserved extracellular matrix structure. The study has shown that a novel method of using supercritical fluid extraction system indeed removed all unnecessary residues and only leaving ECM. The potential of Sc-CO2 de-ECM progressed as a promising approach in tissue repair and regeneration.

Keywords

biologicalbiomedicalbiomaterialbiological synthesis (chemical reaction)membrane

Information

Type
Articles
Information
MRS Advances , Volume 3 , Issue 40: Soft Materials and Biomaterials , 2018 , pp. 2391 - 2397
Copyright
Copyright © Materials Research Society 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

Guler, S. et al. , Tissue Eng Part C Methods, 23, 540–547 (2017)CrossRefGoogle Scholar
Ramot, Y., et al. , Adv. Drug Adv Drug Deliv Rev., 107, 153–162 (2016)CrossRefGoogle Scholar
O’Brien, FJ., Materials today. 14, 88–95 (2011)CrossRefGoogle Scholar
Badylak, SF., Transplant Immunology. 12, 367–377 (2004)CrossRefGoogle Scholar
Vorotnikova, E., et al. , Matrix Biol. 29, 690–700 (2010)CrossRefGoogle Scholar
Brown, BN. et al. , Tissue Engineering. 17, 411-421 (2011)CrossRefGoogle Scholar
Choi, YC. et al. ,Tissue Eng Part C Methods. 18, 866–876 (2012)CrossRefGoogle Scholar
Badylak, SF., Semin Cell Dev Biol. 13, 377–383 (2002)CrossRefGoogle Scholar
Keane, TJ. et al. , Biomaterials, 2016. (Woodhead Publishing, Cambridge, United Kingdom), pp. 75–103Google Scholar
Gilbert, TW. et al. , Biomaterials. 27, 3675–3683 (2006)Google Scholar
Crapo, PM. et al. , Biomaterials. 32, 3233–3243 (2011)CrossRefGoogle Scholar
Keane, TJ. et al. ,Methods. 84, 25–34 (2015)CrossRefGoogle ScholarPubMed
Roosens, A. et al. , Ann Biomed Eng. 44, 2827–2839 (2016)CrossRefGoogle Scholar
Sawada, K. et al. , J Chem Technol Biotechnol. 83, 943–949 (2008)CrossRefGoogle Scholar
Casali, D.M. et al. , J Supercrit Fluids. 131, 72–81 (2018)CrossRefGoogle Scholar
Kim, NS. et al. , MRS Advances, 1–6. doi:10.1557/adv.2018.437 (2018)CrossRefGoogle Scholar