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Creating conjugated hydrogel encapsulated membranes

Published online by Cambridge University Press:  01 February 2011

Tae-Joon Jeon ,
Noah Malmstadt  and
Jacob Schmidt
Tae-Joon Jeon
Affiliation:
UCLA, Department of Bioengineering, 7523 Boelter Hall, 420 Westwood Plaza, Los Angeles, CA, 90095, United States
Noah Malmstadt
Affiliation:
noahmalm@gmail.com, UCLA, Department of Bioengineering, 7523 Boelter Hall, 420 Westwood Plaza, Los Angeles, CA, 90095, United States
Jacob Schmidt
Affiliation:
schmidt@seas.ucla.edu, UCLA, Department of Bioengineering, 7523 Boelter Hall, 420 Westwood Plaza, Los Angeles, CA, 90095, United States
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Abstract

Device engineering for ion channel proteins requires developing systems that incorporate mechanically stable, long-lived lipid bilayer membranes. Building on our previous work, we have further increased lipid bilayer longevity through covalent conjugation of lipid molecules in the bilayer to an encapsulating hydrogel. This is accomplished by polymerizing the hydrogel in situ around a gigaohm-seal membrane containing vinyl-modified lipid head groups, forming a conjugated hydrogel encapsulated membrane (cgHEM). Membranes formed in this manner show remarkable stability, maintaining gigaohm-level resistance for over 270 hours, better than an order-of-magnitude improvement over the previous state of the art. They also demonstrate the capacity to support the incorporation and measurement of ion channel proteins at the single-molecule level.

Keywords

self-assemblysensorbiomimetic (assembly)
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 950: Symposium D – Biosurfaces and Biointerfaces , 2006 , 0950-D09-01
Copyright
Copyright © Materials Research Society 2007

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References

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