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Development of A First-Responder Fluorescence Reader for Microarray Cytokine Assay of Human Immune Response to Disease

Published online by Cambridge University Press:  01 February 2011

David B. Fenner ,
David I Rosen ,
Anthony A Ferrante ,
Amy E Stevens ,
Chad E Bigelow  and
Steven J Davis
David B. Fenner
Affiliation:
Fenner@psicorp.com, Physical Sciences Inc., Photonics and BioMed Tech, 20 New England Business Center, Andover, MA, 01810, United States, 978-738-8205
David I Rosen
Affiliation:
Rosen@psicorp.com, Physical Sciences Inc., Photonics, BioMed and Bio Sciences, 20 New England Business Center, Andover, MA, 01810, United States
Anthony A Ferrante
Affiliation:
Ferrante@psicorp.com, Physical Sciences Inc., Photonics, BioMed and Bio Sciences, 20 New England Business Center, Andover, MA, 01810, United States
Amy E Stevens
Affiliation:
Stevens@psicorp.com, Physical Sciences Inc., Photonics, BioMed and Bio Sciences, 20 New England Business Center, Andover, MA, 01810, United States
Chad E Bigelow
Affiliation:
Bigelow@psicorp.com, Physical Sciences Inc., Photonics, BioMed and Bio Sciences, 20 New England Business Center, Andover, MA, 01810, United States
Steven J Davis
Affiliation:
Davis@psicorp.com, Physical Sciences Inc., Photonics, BioMed and Bio Sciences, 20 New England Business Center, Andover, MA, 01810, United States
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Abstract

Development of field portable apparatus and methods for cytokine assay of human saliva by fluorescent-reporting microarray plates is described.Multiplexed assay of 12 cytokines for minimally-processed saliva is read with a CCD-based imager under LED excitation.Immune responsive cytokines are measured at levels significant for indication of human disease state.The motivational context for the new apparatus development, the general optical design issues, saliva protocol, and image analysis are described.

Keywords

biomedicalopticalsurface reaction

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