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Effect of Glutaraldehyde on Properties of Membranes Preparedfrom Fish Scale Collagen

Published online by Cambridge University Press:  01 March 2012

Zhefeng Xu ,
Toshiyuki Ikoma ,
Tomohiko Yoshioka ,
Motohiro Tagaya ,
Satoshi Motozuka ,
Rena Matsumoto ,
Toshimasa Uemura  and
Junzo Tanaka
Zhefeng Xu
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute Technology University, O-okayama 2-12-1, Meguro-ku, Tokyo 152-8550 JAPAN
Toshiyuki Ikoma
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute Technology University, O-okayama 2-12-1, Meguro-ku, Tokyo 152-8550 JAPAN
Tomohiko Yoshioka
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute Technology University, O-okayama 2-12-1, Meguro-ku, Tokyo 152-8550 JAPAN
Motohiro Tagaya
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute Technology University, O-okayama 2-12-1, Meguro-ku, Tokyo 152-8550 JAPAN
Satoshi Motozuka
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute Technology University, O-okayama 2-12-1, Meguro-ku, Tokyo 152-8550 JAPAN Department of Mechanical Engineering, Gifu National College of Technology, Kamimakuwa 2236-2, Motosu-si, Gifu 501-0495, JAPAN
Rena Matsumoto
Affiliation:
Nanosystem Research Institute, National Institute of Advanced Industrial Science and Technology, Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 3-5-8562, JAPAN
Toshimasa Uemura
Affiliation:
Nanosystem Research Institute, National Institute of Advanced Industrial Science and Technology, Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 3-5-8562, JAPAN
Junzo Tanaka
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute Technology University, O-okayama 2-12-1, Meguro-ku, Tokyo 152-8550 JAPAN
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Abstract

Collagen fibril membranes (CFMs) with a high mechanical property werefabricated with a lateral face evaporation method, in which type Iatelocollagen extracted from tilapia scales was used. The density andthickness of the CFM obtained were 0.51 ± 0.04 mg/cm3 and 50 ± 5μm. The collagen fibrils in the CFM had a similar periodic stripped patternof 67 nm with native collagen fibrils. The CFM was crosslinked in gaseousglutaraldehyde for different duration in order to increase the mechanicalproperty. The crosslinking degrees of the CFMs analyzed by free amino groupsgradually increased to 70.3 % against the exposure duration until 6 hours,and reached a plateau. The denaturation temperatures of the CFMs with thecrosslinking degrees at 20.4 % to 43% were linearly increased from 49°C to75°C. The tensile strength of the CFMs was slightly improved until thecrosslinking degree at 33.3 % and then the tensile strength rapidlyincreased to be 68 MPa. It was suggested that a percolation phenomenon tookplace in the CFMs by crosslinking of collagen fibrils with polymerized GAmolecules.

Keywords

biomaterialchemical synthesismicrostructure

Information

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1418: Symposium LL/MM – Gels and Biomedical Materials , 2012 , mrsf11-1418-mm06-04
Copyright
Copyright © Materials Research Society 2012

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References

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