Hostname: page-component-76fb5796d-9pm4c Total loading time: 0 Render date: 2024-04-30T02:42:14.402Z Has data issue: false hasContentIssue false
  • English
  • Français

Electrostatically Induced Bundle Formation of Rodlike Polyelectrolytes: Comparison of Predictions from Monte Carlo Simulations with Experiments on Fd And M13 Virus Particles.

Published online by Cambridge University Press:  15 February 2011

Lars Nordenskiöld ,
Alexander Lyubartsev ,
Jay X. Tang  and
Paul A. Janmey
Lars Nordenskiöld
Affiliation:
Stockholm University, Physical Chemistry, S-10691 Stockholm, Sweden, lnor@physc.su.se
Alexander Lyubartsev
Affiliation:
Stockholm University, Physical Chemistry, S-10691 Stockholm, Sweden, lnor@physc.su.se
Jay X. Tang
Affiliation:
Div. of Experimental Medicine, Brigham and Women's Hospital, Harvard Medical School, 221 Longwood Ave., LMRC 301, Boston, MA 02115.
Paul A. Janmey
Affiliation:
Div. of Experimental Medicine, Brigham and Women's Hospital, Harvard Medical School, 221 Longwood Ave., LMRC 301, Boston, MA 02115.
Get access

Abstract

This work compares the electrostatic bundling of two Inovirus particles: fd and M13, that are structurally identical except that the effective axial charge density of M 13 is approximately 30% lower than that of fd. The electrostatic force (or osmotic pressure) between these ordered biopolyelectrolytes, as a function of inter-rod separation, has been calculated with Monte Carlo simulations. Comparison is made with experiments on the bundling of fd and M 13 caused by divalent ions, as detected by light scattering. In the theoretical calculations, the bundling results from electrostatic attraction between the neighbouring polyelectrolytes, caused by the correlated interactions between the ion clouds. The importance of this effect (i. e. capacity to induce bundling) is governed by surface charge density of the polyelectrolyte, amount of multivalent ion present, charge and size of the hydrated multivalent ion. These predictions are in very good agreement with the presented experimental results on bundling of fd and M 13 caused by Ca2+ and Mg2+

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 489: Symposium K – Materials Science of the Cell , 1997 , 61
Copyright
Copyright © Materials Research Society 1998

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

[1] Tang, J. X., Wong, S., Tran, P. T. and Janmey, P. A., Ber. Bunsenges. Phys. Chem. 100, 796–806 (1996).Google Scholar
[2] Bloomfield, V. A., Current Opinion in Structural Biology 6, 334–341 (1996).Google Scholar
[3] Anderson, C. F. and Record, M. T. Jr., Annu. Rev. Biophys. Biophys. Chem. 19, 423–465 (1990).Google Scholar
[4] Oosawa, F., Polyelectrolytes, Marcel Dekker, Inc., 1971 Google Scholar
[5] Guldbrand, L., Nilsson, L. and Nordenskiild, L., J. Chem. Phys. 85, 6686–6698 (1986).Google Scholar
[6] Lyubartsev, A. P., and Nordenskiold, L., J.Phys.Chem. 99, 10373–10382 (1995)Google Scholar
[7] Day, L. A., Marzec, C. J., Reisberg, S. A. and Casadevall, A., Ann. Rev. Biophys. Biophys. Chem. 17, 509–539 (1988).Google Scholar
[8] Sambrook, J., Fritsch, E. F. and Maniatis, T., Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor Lab. Press, 1989; Vol.1, pp 4.21.Google Scholar
[9] Tang, J. X., Ito, T., Tao, T., Traub, P., and Janmey, P. A., Biochemistry 36, 12600–12607 (1977)Google Scholar
[10] Paulsen, M. D., Anderson, C. F. and Record, M. T. Jr., Biopolymers 27, 1249–1265 (1988).Google Scholar
[11] Nilsson, L. G., Guldbrand, L. and Nordenskiöld, L., Mol. Phys. 72, 177–192 (1991).Google Scholar
[12] Wilson, R. W. and Bloomfield, V. A., Biochemistry, 18, 2192–2196 (Google Scholar