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Engineered Nanostructured Coatings for Enhanced ProteinAdsorption and Cell Growth

Published online by Cambridge University Press:  27 February 2012

Fereydoon Namavar ,
Alexander Rubinstein ,
Renat F. Sabirianov ,
Geoffrey M. Thiele ,
J. Graham Sharp ,
Utsav Pokharel ,
Roxanna M. Namavar  and
Kevin L. Garvin
Fereydoon Namavar
Affiliation:
Department of Orthopaedic Surgery and Rehabilitation, UNMC, Omaha, NE 68198, U.S.A.
Alexander Rubinstein
Affiliation:
Department of Physics, University of Nebraska, Omaha, NE 68182, U.S.A.
Renat F. Sabirianov
Affiliation:
Department of Physics, University of Nebraska, Omaha, NE 68182, U.S.A.
Geoffrey M. Thiele
Affiliation:
Department of Internal Medicine Rheumatology, UNMC, Omaha, NE 68198, U.S.A.
J. Graham Sharp
Affiliation:
Department of Genetics, Cell Biology and Anatomy, UNMC, Omaha, NE 68198, U.S.A.
Utsav Pokharel
Affiliation:
Department of Orthopaedic Surgery and Rehabilitation, UNMC, Omaha, NE 68198, U.S.A.
Roxanna M. Namavar
Affiliation:
Department of Orthopaedic Surgery and Rehabilitation, UNMC, Omaha, NE 68198, U.S.A.
Kevin L. Garvin
Affiliation:
Department of Orthopaedic Surgery and Rehabilitation, UNMC, Omaha, NE 68198, U.S.A.
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Abstract

We designed and produced pure cubic zirconia (ZrO2) ceramic1coatings by an ion beam assisted deposition (IBAD) with nanostructurescomparable to the size of proteins. Our ceramic coatings exhibit highhardness and a zero contact angle with serum. In contrast to hydroxyapatite(HA), nano-engineered zirconia films possess excellent adhesion to allorthopaedic materials. Cell adhesion and proliferation experiments wereperformed with a bona fide mesenchymal stromal cell line (OMA-AD). Ourexperimental results indicate that the nano-engineered cubic zirconia issuperior in supporting growth, adhesion, and proliferation. Since cellattachment is mediated by adhesive proteins such as fibronectin (FN), toelucidate why cells attach more effectively to our nanostructures, weperformed a comparative analysis of adsorption energies of FN fragment usingquantum mechanical calculations and Monte Carlo (MC) simulation both onsmooth and nanostructured surfaces. We have found that a FN fragment adsorbssignificantly stronger on the nanostructured surface than on the smooth surface2.

Keywords

adhesionadsorptionion-beam assisted deposition

Information

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1418: Symposium LL/MM – Gels and Biomedical Materials , 2012 , mrsf11-1418-mm02-04
Copyright
Copyright © Materials Research Society 2012

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References

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