Hostname: page-component-76dd75c94c-t6jsk Total loading time: 0 Render date: 2024-04-30T07:28:01.534Z Has data issue: false hasContentIssue false
  • English
  • Français

Influence of DSS-8 on the remineralisation of Dentine

Published online by Cambridge University Press:  31 January 2011

Chia-Chan Hsu ,
Elizabeth Marie Hagerman ,
Hsiu-Ying Chung ,
Wenyuan Shi ,
Jenn-Ming Yang  and
Ben Wu
Chia-Chan Hsu
Affiliation:
richardhsu@ucla.edu, University of California Los Angeles, Materials Science and Engineering, Los Angeles, California, United States
Elizabeth Marie Hagerman
Affiliation:
sodium0303@hotmail.com, University of California Los Angeles, Bioengineering, Los Angeles, California, United States
Hsiu-Ying Chung
Affiliation:
richardhsu0303@yahoo.com, Feng Chia University, Materials Science and Engineering, Taichung, Taiwan, Taiwan, Province of China
Wenyuan Shi
Affiliation:
sodium0303@yahoo.com.tw, University of California, Los Angeles, School of Dentistry, Los Angeles, California, United States
Jenn-Ming Yang
Affiliation:
chiachan@seas.ucla.edu, United States
Ben Wu
Affiliation:
pqucla@yahoo.com, United States
Get access

Abstract

Dental remineralization may be achieved by mediating the interactions between tooth surfaces with free ions and biomimetic peptides. We recently developed octuplet repeats of aspartate-serine-serine (DSS-8) peptide, which occurs in high abundance in naturally occurring proteins that are critical for tooth remineralization. In this paper, we evaluated the possible role of DSS-8 in dentin remineralization. Human dentin specimens were demineralized, exposed briefly to DSS-8 solution, and then exposed to concentrated ionic solutions that favor remineralization. Dentin nano-mechanical behaviors, hardness and elastic modulus, at various stages of treatment were determined by nanoindentation. The phase, microstructure and morphology of the resultant surfaces were characterized using grazing incidence X-ray diffraction, variable pressure scanning electron microscopy, and atomic force microscopy, respectively. Nanoindentation results show that DSS-8 remineralization effectively improves the mechanical and elastic properties of native dentin. Moreover, the hardness and elastic modulus for the DSS-8 treated dentin were significantly higher than surfaces remineralized without DSS-8.

Keywords

nano-indentationscanning electron microscopy (SEM)x-ray diffraction (XRD)
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1187: Symposium KK – Structure-Property Relationships in Biomineralized and Biomimetic Composites , 2009 , 1187-KK05-34
Copyright
Copyright © Materials Research Society 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

[1] GWJ, Marshall, SJ, Marshall, JH, Kinney, Balooch, M. The dentin substrate: structure and properties related to bonding. J Dent 1997;25:441–58.Google Scholar
[2] Linde, A, Lundgren, T. From serum to the mineral phase. The role of the odontoblast in calcium transport and mineral formation. Int J Dev Biol 1995;39:213–22.Google Scholar
[3] WT, Butler, Ritchie, H. The nature and functional significance of dentin extracellular matrix proteins. Int J Dev Biol 1995;39:169–79.Google Scholar
[4] WGS, Stetler, Veis, A. Bovine dentin phosphophoryn: calcium ion binding properties of a high molecular weight preparation. Calcif Tissue Int 1987;40(2):97–102.Google Scholar
[5] He, G, Dahl, T, Veis, A, Geroge, A. Nucleation of apatite crystals in vitro by self-assembled dentin matrix protein 1. Nat Mater 2003;2(8):552–8.Google Scholar
[6] CS, Sikes, ML, Yeung, AP, Wheeler. In surface reactive peptides and polymers: discovery and commercialization. Washington: ACS;1991.Google Scholar
[7] SR, Qiu, Wierzbicki, A, CA, Orme, AM, Cody, JR, Hoyer, GH, Nancollas, Zepeda, S, JJD, Yoreo. Molecular modulation of calcium oxalate crystallization by osteopontin and citrate. Proc Natl Acad Sci 2004;101(7):1811–5.Google Scholar
[8] Bigi, A, Boanini, E, Rubini, K, Gazzano, M. Hydroxyapatite nanocrystals modified with acidic amino acids. Eur J Inorg Chem 2006;23: 4821–6.Google Scholar
[9] NI, Papanearchou, Leventouri, T, AC, Kis, Hotiu, A, IM, Anderson. Effect of simulated body fluid on the microstructure of ferrimagnetic bioglass-ceramics. Mater Res Soc Symp Proc 2005;839:371–6.Google Scholar
[10] Kokubo, T, Takadama, H. How useful is SBF in predicting in vivo bone bioactivity. Biomaterials 2006:27:2907.10.1016/j.biomaterials.2006.01.017Google Scholar
[11] WL, Murphy, DJ, Monney. Bioinspired growth of crystalline carbonate apatite on biodegradable polymer substrata. J Am Chem Soc 2002;124(9):1910–7.Google Scholar
[12] JCPDS No. 01–074-0566. International Center for Diffraction Data: Newton Square, PA 2003.Google Scholar
[13] JCPDS No. 00–035-0180. International Center for Diffraction Data: Newton Square, PA; 2003.Google Scholar