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Tailoring nanopores for efficient sensing of different biomolecules

Published online by Cambridge University Press:  01 February 2011

Oukhaled AbdelGhani ,
Laurent Bacri ,
Eric Bourhis ,
Birgetta schiedt ,
Ali Madouri ,
Gilles Patriarche ,
R Jede ,
J.M. Guegan ,
Philippe Guegan  and
Loic Auvray
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Oukhaled AbdelGhani
Affiliation:
oukhaled_ghani@yahoo.frabdelghani.oukhaled@gmail.fr
Laurent Bacri
Affiliation:
laurent.bacri@univ-evry.fr
Eric Bourhis
Affiliation:
eric.bourhis@lpn.cnrs.fr
Birgetta schiedt
Affiliation:
schiedt@mpip-mainz.mpg.de
Ali Madouri
Affiliation:
ali.madouri@lpn.cnrs.fr
Gilles Patriarche
Affiliation:
gilles.patriarche@lpn.cnrs.fr
Philippe Guegan
Affiliation:
philippe.guegan@univ-evry.fr
Loic Auvray
Affiliation:
loic.auvray@univ-evry.fr
Juan Pelta
Affiliation:
juan.pelta@univ-evry.fr
Jacques Gierak
Affiliation:
jacques.gierak@lpn.cnrs.fr
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Abstract

Highly Focused Ion Beams (FIB) are used to produce in one step large quantities of solid state nanopores drilled in thin dielectric films with high reproducibility and well controlled morphologies. We explore both the production of nanopores of various diameters and study their applicability to different biological molecules such as DNA, or folded and unfolded proteins, and then we compare their transport properties. We also report on the translocation of Fibronectin which an original experiment made possible is using the methodology described in this article.

Keywords

ion-beam processingpolymerbiological

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