Hostname: page-component-54dcc4c588-smtgx Total loading time: 0 Render date: 2025-09-15T02:40:35.616Z Has data issue: false hasContentIssue false
  • English
  • Français

Conducting Polymer-based Aptamer Biosensor for in situ Monitoring of Cytokine

Published online by Cambridge University Press:  01 February 2011

Wei Liao ,
Bradly Randall ,
Nicolas Alba  and
Xinyan Tracy Cui
Wei Liao
Affiliation:
wel33@pitt.edu, University of Pittsburgh, Bioengineering, 5065 Biomedical Science Tower 3, 3501 Fifth ave, Pittsburgh, PA, 15260, United States
Bradly Randall
Affiliation:
bar12@pitt.edu, University of Pittsburgh, Bioengineering, Pittsburgh, PA, 15261, United States
Nicolas Alba
Affiliation:
nia16@pitt.edu, University of Pittsburgh, Bioengineering, Pittsburgh, PA, 15261, United States
Xinyan Tracy Cui
Affiliation:
xic11@pitt.edu, University of Pittsburgh, Bioengineering, Pittsburgh, PA, 15261, United States
Get access

Abstract

Neural prostheses often suffer from undesired chronic inflammatory tissue response. Therefore; a sensitive and real-time biosensor for inflammatory cytokine detection would provide invaluable information toward solving this problem. Such a biosensor based on an aptamer probe doped in polypyrrole and subsequent electrochemical impedance spectroscopy (EIS) has been developed. PDGF, an important inflammatory cytokine, has been successfully measured in both offline EIS characterization and real-time impedance monitoring. For in situ detection of PDGF, the best sensitivity of 10 ng/ml has been achieved.

Keywords

biomedicalpolymerelectrochemical synthesis

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

REFERENCES

[1] Schwartz, A. B., Cui, X. T., Weber, D. J., and Moran, D. W., “Brain-controlled interfaces: movement restoration with neural prosthetics,” Neuron, Vol. 52, pp. 205–20, Oct 5 2006.Google Scholar
[2] Schwartz, A. B., “Cortical neural prosthetics,” Annual Review of Neuroscience, Vol. 27, pp. 487–507, 2004.Google Scholar
[3] Liao, W. and Cui, X. Y., “Reagentless aptamer based impedance biosensor for monitoring a neuro-inflammatory cytokine PDGF,” Biosensors & Bioelectronics, Vol. 23, pp. 218–224, 2007.Google Scholar
[4] Cui, X. Y., Hetke, J. F., Wiler, J. A., Anderson, D. J., and Martin, D. C., “Electrochemical deposition and characterization of conducting polymer polypyrrole/PSS on multichannel neural probes,” Sensors and Actuators a-Physical, Vol. 93, pp. 8–18, Aug 25 2001.Google Scholar
[5] Cosnier, S., “Biosensors based on electropolymerized films: new trends,” Analytical and Bioanalytical Chemistry, Vol. 377, pp. 507–520, Oct 2003.Google Scholar
[6] Ramanavicius, A., Ramanaviciene, A., and Malinauskas, A., “Electrochemical sensors based on conducting polymer- polypyrrole,” Electrochimica Acta, Vol. 51, pp. 6025–6037, Aug 25 2006.Google Scholar
[7] Wang, J., Jiang, M., Fortes, A., and Mukherjee, B., “New label-free DNA recognition based on doping nucleic-acid probes within conducting polymer films,” Analytica Chimica Acta, Vol. 402, pp. 7–12, Dec 3 1999.Google Scholar
[8] Liao, W., Guo, S., and Zhao, X. S., “Novel probes for protein chip applications,” Frontiers in Bioscience, Vol. 11, pp. 186–197, Jan 1 2006.Google Scholar
[9] Bartlett, P. N. and Cooper, J. M., “A Review of the Immobilization of Enzymes in Electropolymerized Films,” Journal of Electroanalytical Chemistry, Vol. 362, pp. 1–12, Dec 30 1993.Google Scholar