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Electrical and Chemical Control of Surfaces for DNA Immobilization and Hybridization

Published online by Cambridge University Press:  01 February 2011

Ricardo Cabeca ,
D. M.F. Prazeres ,
V. Chu  and
J. P. Conde
Ricardo Cabeca
Affiliation:
rcabeca@inesc-mn.pt, INESC-MN, INESC-MN, Rua Alves Redol, 9, Lisbon, 1000-029, Portugal
D. M.F. Prazeres
Affiliation:
pcprazer@alfa.ist.utl.pt, IST, IBB, Av. Rovisco Pais, Lisbon, 1049-001, Portugal
V. Chu
Affiliation:
vchu@inesc-mn.pt, INESC-MN, Rua Alves Redol, 9, Lisbon, 1000-029, Portugal
J. P. Conde
Affiliation:
joao.conde@ist.utl.pt, INESC-MN, Rua Alves Redol, 9, Lisbon, 1000-029, Portugal
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Abstract

We present the design of two biointerfaces on a SiO2 substrate for single stranded DNA (ssDNA) immobilization using either covalent grafting or electrostatic adsorption. The influence of the type of biointerface on the rate of diffusion-limited hybridization reaction with complementary ssDNA from a solution is studied. Patterning of the biointerfacefunctionalization layers and the scaling down of the reaction volumes to µL range is demonstrated. The use of externally applied electric field pulses is shown to accelerate the hybridization reaction kinetics to the sub-ms time scale.

Keywords

surface reactionsurface chemistryorganic
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1093: Symposium CC – Designer Biointerfaces , 2008 , 1093-CC04-23
Copyright
Copyright © Materials Research Society 2008

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