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Electrical Impedance Analysis of Phospholipid Bilayer Membranes for Enabling Engineering Design of Bio-based Devices

Published online by Cambridge University Press:  01 February 2011

Stephen A. Sarles
Affiliation:
sarles@vt.edu, Virginia Tech, Mechanical Engineering, 310 Durham Hall, Blacksburg, VA, 24061, United States, 540-231-2902, 540-231-2903
Vishnu B. Sundaresan
Affiliation:
vsundare@vt.edu, Virginia Tech, Mechanical Engineering, 310 Durham Hall, Blacksburg, VA, 24061, United States
Donald J. Leo
Affiliation:
donleo@vt.edu, Virginia Tech, Mechanical Engineering, 310 Durham Hall, Blacksburg, VA, 24061, United States
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Abstract

Recent research at Virginia Tech have shown that active transporter proteins reconstituted into suspended bilayer lipid membranes (BLMs) formed across an array of pores in synthetic substrates can convert chemical energy available in adenosine triphosphate (ATP) into electricity. Experimental results from this work show that this system—called BioCell—is capable of 1.7μW of electrical power per square centimeter of BLM area and per 15μL of ATPase enzyme. In support of such a system, the lipid membrane, as host to active biological proteins and channels, must be formed evenly across a porous substrate, remain stable and yet fluid-like for protein folding and activation, and provide sufficient electrical insulation. We report on the formation and characterization using electrical impedance spectroscopy (EIS) of BLMs formed across two types of porous substrates: polycarbonate filters and single-aperture silicon substrates. Equivalent electrical circuits describing the lipid membranes and their supporting substrates are approximated to fit the measured responses. The results show that BLMs formed in some but not all of the 400nm pores of the filters, while the formation of BLMs on the single-aperture silicon substrates was much more consistent.

Type
Research Article
Copyright
Copyright © Materials Research Society 2008

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References

REFERENCES

1. Sundaresan, V. B., Sarles, S.A., and Leo, D. J., “Chemoelectrical energy conversion of adenosine triphosphate,” Proceedings of SPIE: Active and Passive Smart Structures and Integrated Systems, Vol. 6525 (2007).Google Scholar
2. Sundaresan, V.B., Sarles, S.A., Leo, D. J., and Goode, B.J., “Chemo-electrical energy conversion of adenosine triphosphate in a biological ion transporter,” Smart Dielectric Polymer Properties, Characterization and Their Device: MRS Proceedings, Vol. 949E, 0949–C02 (2006).Google Scholar
3. Hopkinson, D. and Leo, D. J., “Evaluating the mechanical integrity of bilayer lipid membranes using a high-precision pressurization system,” Proceedings of SPIE: Behavior and Mechanics of Multifunctional and Composite Materials, Vol. 6526 (2007).Google Scholar
4. Sarles, S. A., Sundaresan, V. B., and Leo, D. J., “Study of supported bilayer lipid membranes for use in chemo-electric energy conversion via active proton transport,” Proceedings of SPIE: Nanosensing: Materials, Devices, and Systems III, Vol. 6769 (2007).Google Scholar