Skip to main content

Glass Bone Implants: The Effect of Surface Topology on Attachment and Proliferation of Osteoblast Cells on 45S Bioactive Glass

  • Raina Himani Jain (a1), Shaojie Wang (a2), Hassan M. Moawad (a3), Matthias M. Falk (a4) and Himanshu Jain (a5)...

Bioglass 45S is a promising bone implant material with superior biocompatibility. Past research showed that adhesion of bone cells to titanium is strongly affected by its surface architecture. However, little is known about the role of surface topology of glass on its use as an implant. Thus, we systematically investigated the effect of surface roughness (Ra ∼ 0.01 – 1.2 μm) on cell adhesion and proliferation on 45S Bioglass in vitro. MG63 osteosarcoma and MC3T3 osteoblast precursor cells were seeded on the glass samples, and incubated for up to 6 days. The attachment, morphology and proliferation of cells were investigated using fluorescence microscopy. Our results show that cell attachment (as indicated by cell spreading and number of focal adhesion sites), and proliferation rate decrease with increasing roughness of bioactive glass surface. These findings provide important insight for improving surface characteristics of bioactive glass bone implants.

Hide All
1Billotte, W.C., in Biomaterials, Wong, J.Y. and Bronzino, J.D., eds., Chapter 2, CRC Press, (2006).
2Shulman, L.B. Driskell, T.D., ‘Dental Implants: A Historical Perspective Perspective’, in Implants in Dentistry Dentistry, Block, M., Kent, J., Guerra, L., editors, Saunders, W.B., Philadelphia, 1997. Page 6.
3Hench, L.L., ‘The story of Bioglass®’, J Mater Sci: Mater Med 17 (2006) 967978.
4ID, Xynos, AJ, Edgar, LD, Buttery, LL, Hench, JM., Polak Ionic products of bioactive glass dissolution increase proliferation of human osteoblasts and induce insulin insulin-like growth factor II ike mRNA expression and protein synthesis. Biochem Biophys Res Commun. 2000; 276: 461465.
5Hench, L., ‘Bioceramics, Bioceramics,’ J Am Ceram Soc 81 (1998) 17051728.
6Boyan, B., Schwartz, Z., ‘Response of musculoskeletal cells to biomaterials’, J Am Acad Orthop Surg 6 (2006) 157162.
7Levy, S., Van Dalen, M., Agonafer, S., Soboyejo, W., ‘Cell/surface interactions and adhesion on bioactive glass 45S5’, J Mat Sci: Med 18 (2007) 89102.
8Byfield, F., Reen, R., Shentu, T., Levitan, I., Gooch, K.Endot Endothelial actin and cell stiffness is helial modulated by substrate stiffness in 2D and 3D’, J Biomech 42 (2009), 11141119.
9Ziegler, W., Gingras, A., Critchley, D., Emsley, J.Integrin connections to the cytoskeleton through talin and vinculin’, Biochem Soc Trans 36 (2008) 235239.
10Shalabi, M., Gortemaker, A., Van't Hof, M.A., Jansen, J.A., Creugers, N.H.J.Implant Surface Roughness and Bone Healing: a Systematic Review Review’, J Dent Res 85 (2006), 496500.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

MRS Online Proceedings Library (OPL)
  • ISSN: -
  • EISSN: 1946-4274
  • URL: /core/journals/mrs-online-proceedings-library-archive
Please enter your name
Please enter a valid email address
Who would you like to send this to? *



Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed