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A Highly Sensitive and Selective Surface-Enhanced Nanobiosensor

Published online by Cambridge University Press:  01 February 2011

Amanda J. Haes  and
Richard P. Van Duyne
Amanda J. Haes
Affiliation:
Department of Chemistry, Northwestern University, Evanston, IL 60208-3113, USA
Richard P. Van Duyne
Affiliation:
Department of Chemistry, Northwestern University, Evanston, IL 60208-3113, USA
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Abstract

Nanosphere lithography (NSL) derived triangular Ag nanoparticles were used to create an extremely sensitive and specific optical biological and chemical nanosensor. Using simple UV-vis spectroscopy, biotinylated surface-confined Ag nanoparticles were used to detect streptavidin down to one picomolar concentrations. The system was tested for nonspecific binding interactions with bovine serum albumin and was found to display virtually no adverse results. The extremely sensitive and selective response of the Ag nanoparticle sensor indicates an exciting use for biological and chemical sensing.

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