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Ion Channel Sensor on a Silicon Support

  • Michael Goryll (a1), Seth Wilk (a1), Gerard M. Laws (a1), Stephen M. Goodnick (a1), Trevor J. Thornton (a1), Marco Saraniti (a2), John M. Tang (a3) and Robert S. Eisenberg (a3)...


We are building a biosensor based on ion channels inserted into lipid bilayers that are suspended across an aperture in silicon. The process flow only involves conventional optical lithography and deep Si reactive ion etching to create micromachined apertures in a silicon wafer. In order to provide surface properties for lipid bilayer attachment that are similar to those of the fluorocarbon films that are currently used, we coated the silicon surface with a fluoropolymer using plasma-assisted chemical vapor deposition. When compared with the surface treatment methods using self-assembled monolayers of fluorocarbon chemicals, this novel approach towards modifying the wettability of a silicon dioxide surface provides an easy and fast method for subsequent lipid bilayer formation. Current-Voltage measurements on OmpF ion channels incorporated into these membranes show the voltage dependent gating action expected from a working porin ion channel.



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