Skip to main content Accessibility help

Microaligned collagen matrices by hydrodynamic focusing: controlling the pH-induced self-assembly

  • Sarah Köester (a1), Jennie B Leach (a2), Thomas Pfohl (a3) and Joyce Y Wong (a4)

The hierarchical structure of type I collagen fibrils is a key contributor to the mechanical properties of the extracellular matrix (ECM). It is known that the process of in vitro fibrillogenesis strongly depends on the pH of the collagen solution. To date, there are few methods available for precisely controlling and investigating the dependence of collagen fibril assembly on the local pH. The objective of this work was to create highly defined pH gradients to systematically determine the effects of local pH on microscale collagen fibrillogenesis and alignment. We use a microfluidic mixing device to create a diffusion controlled pH gradient, which in turn initiates the self-assembly and concurrent flow-alignment of soluble collagen. Finite element method simulations of the hydrodynamic and diffusive phenomena are used to calculate the local concentrations of the components involved in the reaction. We develop a model to analytically calculate the local pH in the microfluidic device from these concentrations. A comparison with the experimental results from polarized light microscopy are in good agreement with the simulations.

Hide All
[1] Freytes, D.O., Badylak, S.F., Webster, T.J., Geddes, L.A., and Rundell, A.E., Biomaterials 25, 2353 (2004).
[2] Stamm, C., Khosravi, A., Grabow, N., Schmohl, K., Treckmann, N., Drechsel, A., Nan, M., Schmitz, K.P., Haubold, A., and Steinho®, G., Ann. Thorac. Surg. 78, 2084; discussion 2092–2093 (2004).
[3] Boland, E.D., Matthews, J.A., Pawlowski, K.J., Simpson, D.G., Wnek, G.E., and Bowlin, G.L., Front. Biosci. 9, 1422 (2004).
[4] McPherson, J.M., Wallace, D.G., Sawamura, S.J., Conti, A., Condell, R.A., Wade, S., and Piez, K.A., Coll. Relat. Res. 5, 119 (1985).
[5] Christiansen, D.L., Huang, E.K., and Silver, F.H., Matrix Biol. 19, 409 (2000).
[6] Otten, A., Köster, S., Struth, B., Snigirev, A., and Pfohl, T., J. Synchrotron Rad. 12, 745 (2005).
[7] Atkins, P.W.,Physical Chemistry (Oxford University Press, Oxford, 1998), pp. 946948.
[8] Köster, S., Leach, J.B., Struth, B., Pfohl, T., and Wong, J.Y., submitted.
[9] Xia, Y. and Whitesides, G., Ann. Rev. Mat. Sci. 28, 153 (1998).
[10] Pfohl, T., Mugele, F., Seemann, R., and Herminghaus, S.ChemPhysChem 4, 1291 (2003).
[11] Knight, J.B., Vishwanath, A., Brody, J.P., and Austin, R.H., Phys. Rev. Lett. 80, 3863 (1998).
[12] Hong, Z., Chasan, B., Bansil, R., Turner, B.S., Bhaskar, K.R., and Afdhal, N.H., Biomacro-molecules 6, 3458 (2005).
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

MRS Online Proceedings Library (OPL)
  • ISSN: -
  • EISSN: 1946-4274
  • URL: /core/journals/mrs-online-proceedings-library-archive
Please enter your name
Please enter a valid email address
Who would you like to send this to? *



Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed