Skip to main content

Multi-branched gold nanoparticles for Surface Enhanced Raman scattering characterization

  • Jencilin Johnston (a1), Erik N. Taylor (a1) (a2), Richard J. Gilbert (a2) and Thomas J. Webster (a1) (a3)

Surface enhanced Raman scattering (SERS) is a sensitive and reproducible vibrational spectroscopic technique used to detect and characterize molecules near the surface of noble metals like Au, Ag, Pt, Cu, etc. SERS enhances Raman signals through light-induced plasmonic vibrations occurring on irregular metal surfaces and localized electromagnetic augmentation. To better define nano-scale regions of the Raman signal enhancement, we generated gold nanoparticles with a unique multi-branched configuration along with surface-adsorbed fluorescent reporter molecules. The reporter molecules included a set of near-infra red active fluorescent dyes IR820 (green cyanine, photo electronic dye), DTTC (3, 3'-diethylthiatricarbocyanine iodide) and DTDC (3, 3'- diethylthiadicarbocyanine iodide). We employed a one-pot synthesis method in order to generate a stellate configuration in gold nanoparticles through the reduction of HAuCl4 with Good’s buffer, HEPES, at pH 7.4 and room temperature. A cell viability assay was performed with normal esophageal cells exposed to the multi-branched gold nanoparticles and SERS molecules to assess their toxicity. Our results demonstrate the capacity of multibranched gold nanoparticles linked to Raman reporter molecules to generate distinct signature spectra and, with the exception of the gold nanoparticles functionalized with DTTC, remain non-toxic to normal esophageal cells.

Hide All
1. Wang, Y, Yan, B, Chen, L. SERS tags: Novel optical nanoprobes for bioanalysis. Chem Rev. 113(3): 13 91-428, 2013.
2. Abramczyk, H, Brozek-Pluska, B, Surmacki, J, Jablonska-Gajewicz, J, Kordek, R. Raman 'optical biopsy' of human breast cancer. Prog Biophys Mol Biol. 108(1-2):7481, 2012.
3. Xie, J, Zhang, Q, Lee, JY, Wang, DI. The synthesis of SERS-active gold nanoflower tags for in vivo applications. ACS Nano. 2(12):2473–80, 2008.
4. Habib, A, Tabata, M, Wu, YG. Formation of gold nanoparticles by Good’s Buffers: Chemical Society of Japan, 78, 262269, 2005.
5. Moskovits, M. Surface enhanced spectroscopy. Review Modern Physics, 57(3), Part I: 783826, 1985.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

MRS Online Proceedings Library (OPL)
  • ISSN: -
  • EISSN: 1946-4274
  • URL: /core/journals/mrs-online-proceedings-library-archive
Please enter your name
Please enter a valid email address
Who would you like to send this to? *



Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed