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Optical Sol-Gel Materials Based on Binding and Catalysis byBiomolecules

Published online by Cambridge University Press:  21 February 2011

Jeffrey I. Zink ,
Bruce Dunn ,
Stacey Yamanaka ,
Esther Lan ,
J. S. Valentine  and
K. E. Chung
Jeffrey I. Zink
Affiliation:
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90024
Bruce Dunn
Affiliation:
Department of Materials Science and Engineering, University of California, Los Angeles, CA 90024
Stacey Yamanaka
Affiliation:
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90024
Esther Lan
Affiliation:
Department of Materials Science and Engineering, University of California, Los Angeles, CA 90024
J. S. Valentine
Affiliation:
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90024
K. E. Chung
Affiliation:
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90024
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Abstract

The proteins copper-zinc Superoxide dismutase (CuZnSOD), cytochrome c,myoglobin, hemoglobin, and bacterio-rhodopsin are encapsulated in stable,optically transparent, porous, silica glass matrices prepared by the sol-gelmethod such that the biomolecules retain their characteristic reactivitiesand spectroscopic properties. The resulting glasses allow transport of smallmolecules into and out of the glasses at reasonable rates but retain theprotein molecules within their pores. The transparency of the glassesenables the chemical reactions of the immobilized proteins to be monitoredby means of changes in their visible absorption spectra. Silica glassescontaining the immobilized proteins have similar reactivities andspectroscopic properties to those found for the proteins in solution. Theenzymes glucose oxidase and peroxidase were also encapsulated in transparentsilica glass matrices. Upon exposure to glucose solutions, a colored glassis formed that can be used as the active element in a solid state opticallybased glucose sensor. Likewise, gels containing oxalate oxidase andperoxidase exhibit spectroscopic changes upon exposure to aqueous solutionscontaining oxalic acid.

Information

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 346: Symposium N – Better Ceramics Through Chemistry VI , 1994 , 1017
Copyright
Copyright © Materials Research Society 1998

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References

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