The fabrication of nanoporous aluminum oxide (Al2O3) membranes for large scale production of nanowires is performed at room temperature by a two-step anodization of commercially available aluminum foil tapes. During the anodization process, an oxide barrier layer is formed at the interface with aluminum. In the present work, the removal of the barrier is performed by (i) ramping down the voltage with a rate in the range of 0.5 V per 60s to 2 V per 60s and (ii) immersing the substrate in 50% phosphoric acid for up to 5 minutes. Depending on the removal conditions, several morphologies at the oxide-aluminum interface are observed by Scanning Electron Microscopy (SEM). Ramping down the voltage at less than 0.3 V per 15s combined by immersion in 50% phosphoric acid for less than 3 minutes is found to open the barrier layer of the nanopores. The pores have root-like structure with branches as small as few nanometers due to the slow voltage ramping. Several amorphous anodized Al2O3 (AAO) templates with pore diameter ranging from 30 to 40 nm and with length up to 25 μm were prepared by two-step anodization for the cathodic electrodeposition of photoactive nanowire semiconductors such as copper indium diselenide and cadmium sulfide.