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Probing DNA assembly into nanoparticles with shortDNA

Published online by Cambridge University Press:  20 January 2012

Preethi L. Chandran ,
Emilios K. Dimitriadis  and
Ferenc Horkay
Preethi L. Chandran
Affiliation:
Section on Tissue Biophysics and Biomimetics, NICHD, Laboratory of Bioengineering and Physical Science, NIBIB, National Institutes of Health, Bethesda, MD 20892, U.S.A.
Emilios K. Dimitriadis
Affiliation:
Section on Tissue Biophysics and Biomimetics, NICHD,
Ferenc Horkay
Affiliation:
Section on Tissue Biophysics and Biomimetics, NICHD,
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Abstract

DNA is an anionic polyelectrolyte, which occupies a large volume in saltfree solution due to the coulomb repulsion between the charged groups. Inthe presence of high valence cations, DNA condenses into nanoparticles. DNAnanoparticles have generated a lot of interest as a preferred vehicle fordelivering therapeutic DNA in gene therapy. The efficiency of gene deliveryis determined by stability and compactness of the particles. However notmuch is known about the organization of DNA within the particles. The largepolymer cations condense DNA rapidly, with no distinct intermediate stagesthat give insight into the arrangement of DNA within the nanoparticle. Inour work, we form nanoparticles with short DNA strands to slow down thecondensation process. The polymer cation is polyethyleneimine with graftedsugar moieties. Distinct intermediate stages are observed with Atomic ForceMicroscopy. The assembly occurs via the formation of fiber condensates,which appear to be the unit of DNA condensation. Nanoparticles form bycompaction of interweaving networks of fiber condensates.

Keywords

nano-indentationbiologicalscanning probe microscopy (SPM)

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Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1418: Symposium LL/MM – Gels and Biomedical Materials , 2012 , mrsf11-1418-ll02-09
Copyright
Copyright © Materials Research Society 2012

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References

REFERENCES

[1]Santhakumaran, L. M., Chen, A., Pillai, C. K. S., Thomas, T., He, H., and Thomas, T. J., Nanotechnology in Nonviral Gene Delivery: Wiley-VCH Verlag GmbH & Co. KGaA, 2005.Google Scholar
[2]Godbey, W. T., Wu, K. K., and Mikos, A. G., “Poly(ethylenimine) and its role in gene delivery,” Journal of Controlled Release, vol. 60, pp. 149–160, 1999.CrossRefGoogle ScholarPubMed
[3]Lőrincz, O., Tőke, E. R., Somogyi, E., Horkay, F., Chandran, P. L., Douglas, J. F., Szebeni, J., and Lisziewicz, J., “Structure and biological activity of pathogen-like synthetic nanomedicines,” Nanomedicine: nanotechnology, biology, and medicine, 2011.Google Scholar
[4]Giersig, M., Khomutov, G. B., Kasyanenko, N., and Afanasieva, D., “DNA Self-Assembling Nanostructures Induced by Trivalent Ions and Polycations,” in Nanomaterials for Application in Medicine and Biology, ed: Springer Netherlands, 2008, pp. 29–38.Google Scholar
[5]Toke, E. R., Lorincz, O., Somogyi, E., and Lisziewicz, J., “Rational development of a stable liquid formulation for nanomedicine products,” International Journal of Pharmaceutics, vol. 392, pp. 261–267, 2010.Google Scholar
[6]Dunlap, D. D., Maggi, A., Soria, M. R., and Monaco, L., “Nanoscopic structure of DNA condensed for gene delivery,” Nucleic Acids Research, vol. 25, pp. 3095-3101, August 1, 1997 1997.CrossRefGoogle ScholarPubMed
[7]Arscott, P. G., Li, A. Z., and Bloomfield, V. A., “Condensation of DNA by trivalent cations.1. Effects of DNA length and topology on the size and shape of condensed particles,” Biopolymers, vol. 30, pp. 619–630, 1990.Google Scholar
[8]Mann, A., Richa, R., and Ganguli, M., “DNA condensation by poly-l-lysine at the single molecule level: Role of DNA concentration and polymer length,” Journal of Controlled Release, vol. 125, pp. 252–262, 2007.CrossRefGoogle ScholarPubMed