Skip to main content
×
Home
    • Aa
    • Aa

Scattering Studies of the Isolated Red Blood Cell Skeleton, a Biological Two-Dimensional Polymer

  • K. Svoboda (a1) (a2) (a3), C.F. Schmidt (a1) (a3), N. Lei (a4), C.R. Safinya (a4), S.M. Block (a2) (a3) and D. Branton (a2)...
Abstract
Abstract

We studied the conformation of the membrane skeleton of human red blood cells (RBC) after detergent extraction of RBC ghosts, using video microscopy, light scattering, and synchrotronbased small angle X-ray scattering (SAXS). RBC membrane skeletons are two-dimensionally connected, triangulated networks of flexible, polyionic proteins. Immediately after extraction, the skeletons exhibited large-scale thermal undulations and deformed strongly in weak shear flow. Screening of electrostatic repulsion by immersion in high ionic strength buffer led to shrinkage, while the shell-like conformations and the flexibility of the skeletons were preserved. Under high ionic strength conditions (1 M monovalent salt), the static structure factor, S(q), showed two power law regimes S(q) ∝ q −α, with α <≈ 2.0 in the range of wave vectors 4×10−4 Å−1 < g < 8×10−4 Å−1, and α = 2.3 ± 0.1 in the range of wave vectors 8×10−4 Å−1 < q < l×10−1 Å−1. The same power law behavior was observed in low ionic strength buffer (25 mM salt) for q < 2×10−3 Å−1. This result is not consistent with the occurence of a crumpling transition during skeleton shrinkage. The observed form of the static structure factor, with a transition between two regimes with different power law exponents, presents evidence for the theoretically predicted flat phase of 2D-polymers.

Copyright
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

[2] A. Parsegian , N. Fuller , and R. P. Rand , Proc. Natl. Acad. Sci. 76, 2750 (1979); J. N. Israelachvili, Intermolecular and Surface Forces (Academic Press, Orlando, 1985); J. Mahanty and B. W. Ninham, Dispersion Forces (London, 1976).

[3] C. R. Safinya , D. Roux , G. S. Smith , S. K. Sinha , P. Dimon , N. A. Clark , and A. M. Bellocq , Phys. Rev. Lett. 57, 2718 (1986); C. R. Safinya, E. B. Sirota, D. Roux, and G. S. Smith, Phys. Rev. Lett. 62, 1134 (1989); D. Roux, C. R. Safinya, and F. Nallet, in Modern Amphiphilic Physics, edited by Ben-Shaul, W. Gelbart, and D. Roux (Springer), in press.

[4] C. R. Safinya , in Phase Transitions in Soft Condensed Matter, edited by T. Riste and D. Sherrington , Nato ASI series, B, 211, 249 (1989).

[5] P. Basserau , J. Marignan , and G. Porte , J. Phys. (Paris) 48, 673 (1987).

[6] D. R. Nelson and L. Peliti , J. Phys. (Paris) 48, 1085 (1987); J. A. Aronovitz and T. C. Lubensky, Phys. Rev. Let. 60, 2634 (1988); for a broader discussion see: Statistical Mechanics of Membranes and Surfaces, edited by D. R. Nelson, T. Piran, and S. Weinberg (World Scientific, Singapore, 1989).

[8] F. F. Abraham and D. R. Nelson , Science 249, 393 (1990); J. Physique 51, 2653 (1990).

[9] Y. Kantor , M. Kardar , and D. R. Nelson , Phys. Rev. Let. 57, 791 (1986).

[12] G. S. Smith , C. R. Safinya , D. Roux , and N. A. Clark , Mol. Cryst. Liq. Cryst. 144, 235 (1987); G. S. Smith, E. B. Sirota, C. R. Safinya, and N. A. Clark, Phys. Rev. Lett. 60, 813 (1988); E. B. Sirota, G. S. Smith, C. R. Safinya, R. J. Plano, and N. A. Clark, Science 242, 1406 (1988); G. S. Smith, E. B. Sirota, C. R. Safinya, R. J. Plano, and N. A. Clark, J. Chem. Phys. 92, 4519 (1990).

[14] B. J. Stokke , A. Mikkelsen , and A. Elgsaeter , Biochim. Biophys. Acta 816, 102 (1985).

[15] M. L. Sandvold , A. Mikkelsen , and A. Elgsaeter , Acta Chem. Scand. 43, 783 (1989).

[16] A. Elgsaeter , Biochim. Biophys. Acta 536, 235 (1978).

[21] J. T. Dodge , C. Mitchell , and D. J. Hanahan , Arch. Biochem. Biophys. 100, (1963); M. P. Sheetz and D. Sawyer, J. Supramol. Struct. 8, 399 (1978); B. W. Shen, R. Josephs, and T. L. Steck, J. Cell Biol. 102, 997 (1984).

[22] E. Ungewickell and W. Gratzer , Eur. J. Biochem. 88, 379 (1978).

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

MRS Online Proceedings Library (OPL)
  • ISSN: -
  • EISSN: 1946-4274
  • URL: /core/journals/mrs-online-proceedings-library-archive
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 2 *
Loading metrics...

Abstract views

Total abstract views: 36 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 18th August 2017. This data will be updated every 24 hours.