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Separation of DNA Using Ferrofluid Array Electrophoresis

Published online by Cambridge University Press:  10 February 2011

Pascal Mayer ,
Jerôme Bibette  and
J. L. Viovy
Pascal Mayer
Affiliation:
Centre de Recherche Paul Pascal, Av. A. Schweitzer, 33600 PESSAC Present address: GLAXO WELLCOME Research, 14 Ch des Aulx, CP 674, 1228 Plan-les-ouates, GENEVE, SUISSE
Jerôme Bibette
Affiliation:
Centre de Recherche Paul Pascal, Av. A. Schweitzer, 33600 PESSAC
J. L. Viovy
Affiliation:
Laboratoire de Physico Chimie Théorique (URA CNRS 1382) ESPCI, 10 Rue Vauquelin, 75005 PARIS and Laboratoire de Physico-Chimie Curie (UMR CNRS 168), Institut Curie Section de Recherche, 11 Rue P.M. Curie, 75005 (corresponding address)
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Abstract

We present a new method for electrophoretic separation of DNA, Ferro fluid Array Electrophoresis (FAE). The method uses a stabilized suspension of an hydrophobic ferrofluid in aqueous buffer as the separating medium. When this suspension is placed in a slab cell and submitted to a magnetic field perpendicular to the slab plane, it organizes into a regular array of columns with micron-sized spacing. DNA migrating in this maze leads to size-fractionation. Resolution of lambda phage (48.5 kbp) and T4 (140 kbp) DNA molecules in 30 mn is achieved. The motion of individual DNA molecules during FAE is observed using fluorescence videomicroscopy, and the molecular mechanisms responsible for separation are discussed in the light of recent computer simulations. During migration, large DNA molecules temporarily wrap around the impenetrable ferrofluid columns. They disengage by slippage, like a rope on a pulley, and the dependence of the disengagement time upon DNA size is responsible for the size-fractionation.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 463: Symposia EE – Statistical Mechanics in Physics and Biology , 1996 , 57
Copyright
Copyright © Materials Research Society 1997

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References

REFERENCES

1 Flint, D.H. and Harrington, R.E., Biochemistry, 11, 4858 (1972)Google Scholar
2 Duke, T. A. J., Semenov, A. N. and Viovy, J. L., Phys. Rev. Lett. 69, 3260–3263 (1992).Google Scholar
3 Duke, T., Viovy, J.L., Semenov, A.N., Biopolymers 34, 239–247 (1994)Google Scholar
4 Heller, C., Duke, T., Viovy, J.L., Biopolymers, 34, 249–259 (1994)Google Scholar
5 Deutsch, J.M., Phys. Rev. Lett. 59, 1255(1987)Google Scholar
6 Duke, T. A. J. and Viovy, J. L., Phys. Rev. Lett. 68, 542–545 (1992).Google Scholar
7 Zimm, B.H., J. Chem. Phys. 94, 2187 (1991)Google Scholar
8 Smith, S.B., Aldridge, P.K. and Callis, J.B., Science, 243, 203 (1989)Google Scholar
9 Schwartz, D.C. and Koval, M., Nature 338, 520 (1989)Google Scholar
10 Schwartz, D.C. and Cantor, C.R., Cell, 37, 67 (1984)Google Scholar
11 Viovy, J.L., biopolymers, 26, 1929 (1987)Google Scholar
12 Carle, G.F. and Olson, M.V., Trends Biochem. Sci., 12, 284–287 (1987)Google Scholar
13 Turmel, C., Brassard, E., Slater, G.W. and Noolandi, J., Nucleic Acids Res., 569 (1990)Google Scholar
14 Viovy, J.L., Miomandre, F., Miquel, M.C., Caron, F., Sor, F., Electrophoresis, 13, 1–6 (1992)Google Scholar
15 Capillary electrophoresis: an analytical tool in Biotechnology. Righetti, P.G. Ed., Analytical Biotechnology series, CRC press, NY., 1996 (all volume)Google Scholar
16 Mitnik, L., Heller, C., Prost, J., Viovy, J.L., Science, 267, 219–222 (1995)Google Scholar
17 Volkmuth, W.D. and Austin, R.H., Nature, 358, 600 (1992)Google Scholar
18 Bibette, J., J. Magn. Magn. Mater., 122, 37 (1993)Google Scholar
19 Liu, J., Lawrence, E.M., Wu, A., Ivey, M.L., Flores, G.A., Javier, K., Bibette, J. and Richard, J., Phys. Rev. Lett., 74, 2828 (1995)Google Scholar
20 Harrison, D.J., Fluri, K., Seiler, K., Fan, Z., Effenhauser, C.S. and Manz, A., Science, 261, 895 (1993)Google Scholar
21 Nixon, G.I. and Slater, G.W., Phys. Rev. E, 50, 5033 (1994)Google Scholar
22 Volkmuth, W.D., Duke, T., Wu, M.C., Austin, R.H. and Szabo, A., Phys. Rev. Lett. 72, 2117 (1994)Google Scholar
23 Sevick, E.M. and Williams, R.M., Phys. Rev. Lett., 76, 2595 (1996)Google Scholar
24 Long, D., Viovy, J.L. and Ajdari, A., Phys. Rev. Lett., 76, 3858 (1996)Google Scholar