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Sperm Performance Better on Diamond than on Polystyrene

  • Andrei P. Sommer (a1), Dan Zhu (a1) (a2), Friedrich Gagsteiger (a2) and Hans-Jörg Fecht (a1)
Abstract
ABSTRACT

Recently we postulated that polystyrene Petri dishes become soft when in contact with an aqueous milieu. Specifically, we assumed that the effect is restricted to a superficial nanolayer, a condition presumably favoring the establishment of a stable nanolayer of reactive oxygen species (ROS) at the liquid/solid-interface. Cells are known to be hypersensitive to ROS. Previously we used P19 mouse embryonal carcinoma cells and systematically analyzed their capability to climb different substrates placed vertically into a Petri dish. The worst and best performance was found on polystyrene (Petri dish material) and nanocrystalline diamond, respectively. Polystyrene Petri dishes are today standard in laboratories conducting in vitro fertilization (IVF). Here we proceed and extend the investigation to human spermatozoa and show that their performance (vitality) on polystyrene Petri dishes is low compared to that on diamond Petri dishes. This work may propel further research and inspire the development of a new generation of cell-friendly Petri dishes.

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Corresponding author
*Email: andrei.sommer@uni-ulm.de
References
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1.Sommer AP, Zhu D, Brühne K. Surface conductivity on hydrogen-terminated nanocrystalline diamond: Implication of ordered water layers. Cryst. Growth Des. 7, 22982301, 2007.
2.Sommer AP, Zhu D, Fecht HJ. Genesis on diamonds. Cryst. Growth Des. 8, 26282629, 2008.
3.Sommer AP, Zhu D, Wiora M, Fecht HJ. The top of the biomimetic triangle. J. Bionic Eng. 5, 9194, 2008.
4.Sommer AP, Zhu D, Franke RP, Fecht HJ. Biomimetics: Learning from diamonds. J. Mater. Res. 23, 31483152, 2008.
5.Sommer AP, Zhu D, Scharnweber T, Fecht HJ. On the social behaviour of cells. J. Bionic Eng. 7, 15, 2010.
6.Cohen J. Fertility Magazine 6, 1224.
7.WHO laboratory manual for the examination and processing of human semen. Fifth edition, WHO Press, Geneva, Switzerland, 2010.
8.Sommer AP, Zhu D, Scharnweber T, Fecht HJ. Body building on diamonds. J. Bionic Eng. 6, 1417, 2009.
9.Wobus AM, Kleppisch T, Maltsev V, Hescheler J. Cardiomyocyte-like cells differentiated in vitro from embryonic carcinoma cells P19 are characterized by functional expression of adrenoceptors and Ca2+ channels. In Vitro Cell Dev. Biol. Anim. 30A, 425434, 1994.
10.Chen QZ, Harding SE, Ali NN, Lyon AR, Boccaccini AR. Biomaterials in cardiac tissue engineering: Ten years of research survey. Mat. Sci. Eng. R 59, 137, 2008.
11.Abercrombie M, Heaysman JE. Invasiveness of sarcoma cells. Nature 174, 697698, 1954.
12.Abercrombie M, Heaysman JE, Karthauser HM. Social behaviour of cells in tissue culture. III. Mutual influence of sarcoma cells and fibroblasts. Exp. Cell Res. 13, 276291, 1957.
13.Ben-Jacob E, Coffey DS, Levine H. Bacterial survival strategies suggest rethinking cancer cooperativity. Trends Microbiol. 20, 403410, 2012.
14.Wagner W, Saffrich R, Wirkner U, Eckstein V, Blake J, Ansorge A, Schwager C, Wein F, Miesala K, Ansorge W, Ho AD. Hematopoietic progenitor cells and cellular microenvironment: behavioral and molecular changes upon interaction. Stem Cells 23, 11801191, 2005.
15.Sommer AP, Haddad MK, Fecht HJ. It is time for a change: Petri dishes weaken cells. J. Bionic Eng. 9, 353357, 2012.
16.Dumé B. Nanodiamond coating for a better Petri dish. Nanotechweb.org Oct 15, 2012.
17.Krauss S, Zhang CY, Lowell BB. The mitochondrial uncoupling-protein homologues. Nat. Rev. Mol. Cell Biol. 6, 248261, 2005.
18.Discher DE, Mooney DJ, Zandstra PW. Growth factors, matrices, and forces combine and control stem cells. Science 324, 16731677, 2009.
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