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The Use of Electrospun Polycaprolactone as a Dermal Scaffold for Skin Tissue Engineering

  • Ming Chen (a1), Manisha Chopra (a2) and Sankha Bhowmick (a3)

Rapid healing of acute and chronic skin defects is an important objective. In the present work, we report on the design and feasibility of a co-culture system for fibroblasts and keratinocytes by using electrospun polycaprolactone (PCL) scaffolds. Specifically, we quantified the effect of scaffold fiber diameter on keratinocyte attachment, proliferation and differentiation along with collagen secretion by fibroblasts post vacuum seeding with fibroblasts at various depths. The results show that fibroblasts secrete more collagen and keratinocytes differentiate more on 400 nm scaffolds than on 1000 nm scaffolds. Also, fibroblasts co-cultured with keratinocytes provide increased collagen secretion and keratinocyte differentiation. These results suggest that the fiber architecture can be a useful parameter in skin tissue engineering.

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1Lan, C. W., Wang, F. F. and Wang, Y. J., J Biomed Mater Res A 66, 38 (2003).
2Li, W. J., Laurencin, C. T., Caterson, E. J., Tuan, R. S. and Ko, F. K., J Biomed Mater Res 60, 613 (2002).
3Boland, E. D., Wnek, G. E., Simpson, D. G., Pawlowski, K. J. and Bowlin, G. L. J. Macromol. Sci. Pure Appl. Chem. A38, 1231 (2001).
4Murugan, R. and Ramakrishna, S., Tissue Eng 12, 435 (2006).
5Basson, M. D., Modlin, I. M., Turowski, G. and Madri, J. A., Eur J Gastroenterol Hepatol 5, 21 (1993).
6Carsin, H., Ainaud, P., Le Bever, H., Rives, J., Lakhel, A., Stephanazzi, J., Lambert, F. and Perrot, J., Burns 26, 379 (2000).
7Phillips, T. J. and Gilchrest, B. A., Epithelial Cell Biol 1, 39 (1992).
8Rippke, F., Schreiner, V. and Schwanitz, H. J., Am J Clin Dermatol 3, 261 (2002).
9Ojeh, N., Frame, J. and Navsaria, H., Tissue Eng 7, 457 (2001).
10El-Ghalbzouri, A., Lamme, E.N., Blitterswijk, C. van, Koopman, J. and Ponec, M., Biomaterials 25, 2987 (2004).
11Black, A. F., Bouez, C., Perrier, E., Schlotmann, K., Chapuis, F., Damour, O, Tissue Eng 11, 723 (2005).
12Coombes, A. G., Verderio, E., Shaw, B., Li, X., Griffin, M. and Downes, S., Biomaterials 23, 2113 (2002).
13Schwope, A. D., Wise, D. L., Sell, K. W., Skornik, W. A. and Dressler, D. P., Trans, D. P. Am Soc Artif Intern Organs 20A, 103 (1974).
14Bell, E., Ehrlich, H. P., Buttle, D. J. and Nakatsuji, T.. Science 211, 1052 (1981).
15Parenteau, N., Cambridge, (Cambridge University Press, U.K. 1994) p45.
16Chen, M., Patra, P. K., Warner, S. B. and Bhowmick, S., Biophys. Rev. Lett 1, 189 (2006).
17Chen, M., Michaud, H. and Bhowmick, S., J Biomech Eng 131, 074521.1 (2009).
18Chen, M., Patra, P. K., Warner, S. B. and Bhowmick, S., Tissue Eng 13, 579 (2007)
19Chen, M., Patra, P. K., Lovett, M. L., Kaplan, D. L. and Bhowmick, S., J Tissue Eng Regen Med 3, 269 (2009).
20Lim, I. J., Phan, T.T., Bay, B. H., Qi, R., Huynh, H. and Tan, W. T., Lee, S. T. and Longaker, M. T., Am J Physiol Cell Physiol 283, C212 (2002).
21Rheinwald, J. G., Green, H., Cell 6, 331 (1975).
22Kuroyanagi, Y., Kenmochi, M., Ishihara, S., Takeda, A., Shiraishi, A., Ootake, N., Uchinuma, E., Torikai, K., and Shioya, N., Ann Plast Surg 31, 340 (1993)
23Rubin, J. S., Osada, H., Finch, P. W., Taylor, W. G., Rudikoff, S. and Aaronson, S. A., Proc Natl Acad Sci U S A 86, 802 (1989)
24Marchese, C., Rubin, J., Ron, D., Faggioni, A., Torrisi, M. R., Messina, A., A, L. Frati, and Aaronson, S. A., J Cell Physiol 144, 326 (1990).
25Lee, D. Y., Lee, J. H., Lee, E. S., Cho, K. H. and Yang, J. M., Arch Dermatol Res 294, 444 (2003).
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